Lipidomic Analysis of <i>Clostridium cadaveris</i> and <i>Clostridium fallax</i>

Guan, Ziqiang; Garrett, Teresa A.; Goldfine, Howard

doi:10.1002/lipd.12181

Cited by 6 publications

(4 citation statements)

References 34 publications

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“…The unique modified glycerolipids of Gram-positive bacteria are dihexosyl DGs (DHDGs) serving as precursors of membrane lipoteichoic acids (LTA) [22,[57][58][59][60][61]. We monitored these unique glycolipids in all Gram-positive bacteria, as well as lipoteichoic acid precursor (LTAP) 30:4 (Figure 3; Supplementary Tables).…”

Section: Glycerolipids (Gl) and Modified-glsmentioning

confidence: 99%

Comparative Lipidomics of Oral Commensal and Opportunistic Bacteria

Wood,

Le,

Palazzolo

2024

Metabolites

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The oral cavity contains a vast array of microbes that contribute to the balance between oral health and disease. In addition, oral bacteria can gain access to the circulation and contribute to other diseases and chronic conditions. There are a limited number of publications available regarding the comparative lipidomics of oral bacteria and fungi involved in the construction of oral biofilms, hence our decision to study the lipidomics of representative oral bacteria and a fungus. We performed high-resolution mass spectrometric analyses (<2.0 ppm mass error) of the lipidomes from five Gram-positive commensal bacteria: Streptococcus oralis, Streptococcus intermedius, Streptococcus mitis, Streptococcus sanguinis, and Streptococcus gordonii; five Gram-positive opportunistic bacteria: Streptococcus mutans, Staphylococcus epidermis, Streptococcus acidominimus, Actinomyces viscosus, and Nanosynbacter lyticus; seven Gram-negative opportunistic bacteria: Porphyromonas gingivalis. Prevotella brevis, Proteus vulgaris, Fusobacterium nucleatum, Veillonella parvula, Treponema denticola, and Alkermansia muciniphila; and one fungus: Candida albicans. Our mass spectrometric analytical platform allowed for a detailed evaluation of the many structural modifications made by microbes for the three major lipid scaffolds: glycerol, sphingosine and fatty acyls of hydroxy fatty acids (FAHFAs).

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Section: Glycerolipids (Gl) and Modified-glsmentioning

confidence: 99%

Comparative Lipidomics of Oral Commensal and Opportunistic Bacteria

Wood,

Le,

Palazzolo

2024

Metabolites

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“…Alanyl-PE Bacillus subtilis [13] Lysyl-PE Bacillus subtilis [13] PE Glycerol Acetals Clostridium fallax but not C. cadaveris [43][44][45] GPCR Ligands Oral Commensal bacteria [5,9] 1.2. Gram-Negative Bacteria 1.2.1.…”

Section: Lipid Class Bacterial Strains Referencesmentioning

confidence: 99%

Construction of a Bacterial Lipidomics Analytical Platform: Pilot Validation with Bovine Paratuberculosis Serum

Wood

Erol

2023

Metabolites

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Lipidomics analyses of bacteria offer the potential to detect and monitor infections in a host since many bacterial lipids are not present in mammals. To evaluate this omics approach, we first built a database of bacterial lipids for representative Gram-positive and Gram-negative bacteria. Our lipidomics analysis of the reference bacteria involved high-resolution mass spectrometry and electrospray ionization with less than a 1.0 ppm mass error. The lipidomics profiles of bacterial cultures clearly distinguished between Gram-positive and Gram-negative bacteria. In the case of bovine paratuberculosis (PTB) serum, we monitored two unique bacterial lipids that we also monitored in Mycobacterium avian subspecies PTB. These were PDIM-B C82, a phthiodiolone dimycocerosate, and the trehalose monomycolate hTMM 28:1, constituents of the bacterial cell envelope in mycolic-containing bacteria. The next step will be to determine if lipidomics can detect subclinical PTB infections which can last 2-to-4 years in bovine PTB. Our data further suggest that it will be worthwhile to continue building our bacterial lipidomics database and investigate the further utility of this approach in other infections of veterinary and human clinical interest.

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“…Water-insoluble polysaccharides, consumed as prebiotics, isolated from the sclerotium of Poria cocos , can increase butyrate production by increasing the abundance of Lachnospiraceae, Clostridium XIV A and IV , thereby enhancing the integrity of the intestinal mucosa [ 72 ]. The abundance of Clostridium XIV A and IV has been shown to negatively correlate with liver steatosis [ 86 ]. In rats with liver cirrhosis induced by carbon tetrachloride (CCl 4 ), Artesunate (an extract of Artemisia annua ) has been shown to increase levels of Lactobacillus and Eubacterium , thereby reducing the concentration of proinflammatory IL-6 and TNF- α , reducing injury to the intestinal mucosa, and decreasing the numbers of translocated bacteria [ 77 ].…”

Section: Intervention Therapy Using Traditional Chinese Medicine (Tcm)mentioning

confidence: 99%

Intestinal Microbiota and Liver Diseases: Insights into Therapeutic Use of Traditional Chinese Medicine

Wang

Huang²,

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Liver disease is a leading cause of global morbidity and mortality, for which inflammation, alcohol use, lipid metabolic disorders, disturbance to bile acid metabolism, and endotoxins are common risk factors. Traditional Chinese Medicine (TCM) with its “holistic approach” is widely used throughout the world as a complementary, alternative therapy, due to its clinical efficacy and reduced side effects compared with conventional medicines. However, due to a lack of reliable scientific evidence, the role of TCM in the prevention and treatment of liver disease remains unclear. Over recent years, with the rapid development of high-throughput sequencing, 16S rRNA detection, and bioinformatics methodology, it has been gradually recognized that the regulation of intestinal microbiota by TCM can play a substantial role in the treatment of liver disease. To better understand how TCM regulates the intestinal microbiota and suppresses liver disease, we have reviewed and analyzed the results of existing studies and summarized the relationship and risk factors between intestinal microbiota and liver disease. The present review summarizes the related mechanisms by which TCM affects the composition and metabolites of the intestinal microbiome.

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Lipidomic Analysis of Clostridium cadaveris and Clostridium fallax

Cited by 6 publications

References 34 publications

Comparative Lipidomics of Oral Commensal and Opportunistic Bacteria

Comparative Lipidomics of Oral Commensal and Opportunistic Bacteria

Construction of a Bacterial Lipidomics Analytical Platform: Pilot Validation with Bovine Paratuberculosis Serum

Intestinal Microbiota and Liver Diseases: Insights into Therapeutic Use of Traditional Chinese Medicine

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