Lipidomic analysis of platelet senescence

Ruebsaamen, Katharina; Liebisch, Gerhard; Boettcher, Alfred; Schmitz, Gerd

doi:10.1111/j.1537-2995.2010.02584.x

Cited by 30 publications

(46 citation statements)

References 55 publications

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“…Lipid extraction from PL‐EVs (corresponding to 100 μg of total protein), lipid processing, and quantification by lipid mass spectrometry of cholesterol, glycerophospholipids, and major sphingolipids were carried out as earlier (http://onlinelibrary.wiley.com/doi/10.1111/trf.12874/suppinfo). Minor sphingolipids and glycerophospholipids including cardiolipin (CL) and bis(monoacylglycero)phosphate were analyzed by hydrophilic interaction according to Scherer and colleagues .…”

Section: Methodsmentioning

confidence: 99%

Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets

Pienimaeki‐Roemer¹,

Kuhlmann

Böttcher³

et al. 2014

Transfusion

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105

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Differential lipid and protein compositions of PL-EVs suggest their unique cellular origins and functions, partly overlapping with PLT granule secretion. Dense PL-MVs might represent autophagic vesicles released during PLT activation and apoptosis and PL-EXs resemble lipid rafts, with a potential role in PLT aggregation and immunity. Segregation of α-synuclein and Aβ precursor protein, ApoE, and ApoJ into less dense and dense PL-MVs, respectively, show their differential carrier role of neurologic disease-related cargo.

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Section: Methodsmentioning

confidence: 99%

Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets

Pienimaeki‐Roemer¹,

Kuhlmann

Böttcher³

et al. 2014

Transfusion

Self Cite

105

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“…Lipids also contribute to platelet senescence [212], hemostasis, and other disease states [213-216]. Platelet phospholipids, sphingolipids, lysophosphatidic acid, and platelet-activating factor also play an important role in platelet regeneration therapy that involves platelet-rich plasma injections [217, 214, 218-220].…”

Section: Platelet Lipidomics and Bioactive Lipidsmentioning

confidence: 99%

Platelets and cancer: a casual or causal relationship: revisited

Menter

Tucker

Kopetz

et al. 2014

Cancer Metastasis Rev

279

256

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Human platelets arise as subcellular fragments of megakaryocytes in bone marrow. The physiologic demand, presence of disease such as cancer, or drug effects can regulate the production circulating platelets. Platelet biology is essential to hemostasis, vascular integrity, angiogenesis, inflammation, innate immunity, wound healing, and cancer biology. The most critical biological platelet response is serving as “First Responders” during the wounding process. The exposure of extracellular matrix proteins and intracellular components occurs after wounding. Numerous platelet receptors recognize matrix proteins that trigger platelet activation, adhesion, aggregation, and stabilization. Once activated, platelets change shape and degranulate to release growth factors and bioactive lipids into the blood stream. This cyclic process recruits and aggregates platelets along with thrombogenesis. This process facilitates wound closure or can recognize circulating pathologic bodies. Cancer cell entry into the blood stream triggers platelet-mediated recognition and is amplified by cell surface receptors, cellular products, extracellular factors, and immune cells. In some cases, these interactions suppress immune recognition and elimination of cancer cells or promote arrest at the endothelium, or entrapment in the microvasculature, and survival. This supports survival and spread of cancer cells and the establishment of secondary lesions to serve as important targets for prevention and therapy.

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“…LC/MS/MS has been applied to quantitation of eicosanoids, such as thromboxane B2 (TXB 2 ) and HETE, and to identification of SP molecular species 25, 26 (Figure 4 A). Shotgun methods have been applied to the characterization of platelet PLs, lysoPLs, CEs and ceramides in a small number of studies 27-29 . Using traditional methods, individual lipid pools first had to be isolated using TLC, then hydrolyzed to release FAs for determination as free acid species, a considerably slower approach that requires more material 1-4 .…”

Section: Introductionmentioning

confidence: 99%

“…Using traditional methods, individual lipid pools first had to be isolated using TLC, then hydrolyzed to release FAs for determination as free acid species, a considerably slower approach that requires more material 1-4 . Recently, the composition of stored platelets and extracellular vesicles were characterized, showing that vesicles became enriched with LPA, cholesterol, and other lipids 28, 29 .…”

Section: Introductionmentioning

confidence: 99%

Platelet Lipidomics

O'Donnell

Murphy

Watson³

2014

Circulation Research

134

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Lipids are diverse families of biomolecules that perform essential structural and signaling roles in platelets. Their formation and metabolism is tightly controlled by enzymes and signal transduction pathways, and their dysregulation leads to significant defects in platelet function and disease. Platelet activation is associated with significant changes to membrane lipids, and formation of diverse bioactive lipids that play essential roles in hemostasis. In recent years, new generation mass spectrometry analysis of lipids (termed “lipidomics”) has begun to alter our understanding of how these molecules participate in key cellular processes. While, the application of lipidomics to platelet biology is still in its infancy, seminal earlier studies have shaped our knowledge of how lipids regulate key aspects of platelet biology, including aggregation, shape change, coagulation and degranulation, as well as how lipids generated by platelets influence other cells, such as leukocytes and the vascular wall, and thus how they regulate hemostasis, vascular integrity and inflammation, as well as contribute to pathologies including arterial/deep vein thrombosis and atherosclerosis. This review will provide a brief historical perspective on the characterization of lipids in platelets, then an overview of the new generation lipidomic approaches, their recent application to platelet biology, and future perspectives for research in this area. The major platelet-regulatory lipid families, their formation, metabolism, and their role in health and disease, will be summarized.

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Lipidomic analysis of platelet senescence

Cited by 30 publications

References 55 publications

Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets

Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets

Platelets and cancer: a casual or causal relationship: revisited

Platelet Lipidomics

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