Lipidomic analysis to reveals therapeutic effects of herbal cake-separated moxibustion on high-fat diet-induced hyperlipidemia rabbits

Abstract

Background Herbal cake-separated moxibustion (HM) is one of the characteristic therapies for the prevention and treatment of hyperlipidemia (HLP). However, the effect of HM on plasma lipid metabolism in HLP rabbits is not clear. Methods New Zealand rabbits were fed with high-fat diet for 8 weeks to induce HLP model, and then HM was intervened for 8 weeks. The level of blood lipid in serum of rabbits was detected by full biochemical analyzer, and the pathological changes of liver tissue were observed by oil red O staining. Then we used ultra-high performance liquid chromatography / quadrupole time-of-flight mass spectrometry combined with multivariate statistical analysis for non-targeted lipidomic analysis. Results HM ameliorated hyperlipidemia induced the abnormal blood lipid level and improved liver lipid deposition induced by high cholesterol diet. Non-targeted lipidomic analysis showed that HM changed the lipid metabolism profile of HLP rabbits.