2020
DOI: 10.1101/2020.04.08.033100
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Lipidomic and Ultrastructural Characterization of Cell Envelope ofStaphylococcus aureusGrown in the Presence of Human Serum

Abstract: 26Staphylococcus aureus can incorporate exogenous straight-chain unsaturated and 27 saturated fatty acids (SCUFAs and SCFAs, respectively) to replace some of the normally 28 biosynthesized branched-chain fatty acids and SCFAs. In this study, the impact of human serum 29 on the S. aureus lipidome and cell envelope structure was comprehensively characterized. When 30 grown in the presence of 20% human serum, typical human serum lipids, such as cholesterol, 31 sphingomyelin, phosphatidylethanolamines, and phos… Show more

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Cited by 3 publications
(9 citation statements)
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“…Overall, this work demonstrates that culture medium can significantly alter the membrane lipid composition of major human streptococcal pathogens. This work, coupled with a recent study by Hines et al demonstrating S. aureus membrane remodelling in response to human serum [43], supports the significance of culturing pathogens in media that more closely represent the host environments in which the pathogens are found. Further investigation into the biosynthesis and mechanistic roles of PC in the membrane will probably provide novel insights into the pathogen-host interactions and pathogenesis of these streptococcal species.…”
Section: Discussionsupporting
confidence: 77%
“…Overall, this work demonstrates that culture medium can significantly alter the membrane lipid composition of major human streptococcal pathogens. This work, coupled with a recent study by Hines et al demonstrating S. aureus membrane remodelling in response to human serum [43], supports the significance of culturing pathogens in media that more closely represent the host environments in which the pathogens are found. Further investigation into the biosynthesis and mechanistic roles of PC in the membrane will probably provide novel insights into the pathogen-host interactions and pathogenesis of these streptococcal species.…”
Section: Discussionsupporting
confidence: 77%
“…The SCUFAs present in the total fatty acid profile of the CONS probably represent a mixture of SCUFAs truly incorporated into glycerolipids [7,19,22], and the association of serum lipids with the cell surface of the CONS. Lipidomic and ultrastructural analysis of S. aureus grown in the presence of human serum showed that serum lipids become associated with the cell surface and resist washing away with saline solution, but are removeable to a significant extent with Triton X-100 [22]. Kenanian et al [21] showed that growth in serum allows S. aureus to bypass FASII inhibitors through the incorporation of fatty acids derived from host lipids.…”
Section: Growth In Serum Leads To Significant Amounts Of Straight-chain Unsaturated Fatty Acids In the Fatty Acid Profilementioning
confidence: 99%
“…Interpretation of the anisotropy determinations in serum-grown cells is more difficult given the likely association of serum-lipids with the cell surface [22]. In cells grown in TSB or MHB, which do not have surface-associated lipids, we can be confident that 1,6-diphenyl-1,3,5-hexatriene (DPH) inserts into the cytoplasmic membrane and reports the status of the membrane.…”
Section: Increased Membrane Fluidity In Mueller Hinton Broth-and Serum-grown Coagulase-negative Staphylococcal Speciesmentioning
confidence: 99%
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