Lipidomics and RNA-Seq Study of Lipid Regulation in Aphis gossypii parasitized by Lysiphlebia japonica

Abstract

The cotton–melon aphid, Aphis gossypii Glover, is a major insect pest worldwide. Lysiphlebia japonica (Ashmead) is an obligate parasitic wasp of A. gossypii, and has the ability to regulate lipid metabolism of the cotton-melon aphid. Lipids are known to play critical roles in energy homeostasis, membrane structure, and signaling. However, the parasitoid genes that regulate fat metabolism and lipid composition in aphids are not known. 34 glycerolipids and 248 glycerophospholipids were identified in this study. We have shown that a 3-day parasitism of aphids can induce significant changes in the content and acyl chain composition of triacylglycerols (TAGs) and subspecies composition of glycerophospholipids content and acyl chains. It also upregulate the expression of several genes involved in triacylglycerol synthesis and glycerophospholipid metabolism. Pathway analysis showed that a higher expression of genes involved in the tricarboxylic acid cycle and glycolysis pathways may contribute to TAGs synthesis in parasitized aphids. Interestingly, the higher expression of genes in the sphingomyelin pathway and reduced sphingomyelin content may be related to the reproductive ability of A. gossypii. We provide a comprehensive resource describing the molecular signature of parasitized A. gossypii particularly the changes associated with the lipid metabolism and discuss the biological and ecological significance of this change.

De novo characterization of Phenacoccus solenopsis transcriptome and analysis of gene expression profiling during development and hormone biosynthesis

“…According to the KEGG pathway analysis, fifteen, six, and eleven pathways were observed as significantly enriched for DEGs of EggI vs. second instar, second vs. third instar, and third instar vs. adult females, respectively. The pathway enriched for different developmental stages were glycerolipid metabolism 69 , FoxO-signaling pathway 20 , 70 – 73 , Hippo signaling pathway 74 – 76 , fatty acid biosynthesis 77 – 79 , biosynthesis of amino acids 41 , 80 – 85 and steroid biosynthesis 86 – 88 suggesting that genes in these pathways seem to be involved in early developmental process. Likewise, we have identified all the genes coding for enzymes involved in juvenile and ecdysteroid hormone biosynthetic pathways and found many of them as potential RNAi targets for silencing (Fig.…”

Molecular Evidence that Lysiphlebia japonica Regulates the Development and Physiological Metabolism of Aphis gossypii

“…The transcriptome data was obtained from our previous experiments, which have the ArrayExpress accession number is MTAB-5228 [ 55 ]. Proteome and transcriptome data were obtained from the same samples at the same time.…”

Symbiotic bracovirus of a parasite manipulates host lipid metabolism via tachykinin signaling

“…maturity [77][78][79][80][81]. For example, Lysiphlebia japonica parasitization causes a sharp increase in TG levels in the body of the Aphis gossypii host, which is necessary for L. japonica growth and reproduction [80]; similarly, the parasitoid Chelonus inanitus also causes an accumulation of lipids in the whole body of the Spodoptera littoralis host, which is crucial for parasitoid survival [82,83].…”