Lipidomics of equine amniotic fluid: Identification of amphiphilic (O-acyl)-ω-hydroxy-fatty acids

Wood, Paul L.; Ball, Barry A.; Scoggin, Kirsten E.; Troedsson, Mats H.T.; Squires, E.L.

doi:10.1016/j.theriogenology.2017.09.012

Cited by 24 publications

(18 citation statements)

References 29 publications

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“…The predominant OAHFAs were OAHFA 48:2 and OHFA 50:2 The fatty acid constituents (fatty acid/ ω-hydroxy-fatty acid) of these lipids were determined by MS 2 (<2 ppm mass error). Each of the OAHFAs had several isobaric forms with OHFA 48:2 mass including OAHFA 16:1/32:1(OH) and OAHFA 18:1/30:1(OH) while the mass for OHFA 50:2 included OAHFA 18:1/32:1(OH) and OAHFA 16:1/34:1(OH), similar to our previous observations with equine sperm [15] and amniotic fluid [16]. Cholesterol sulfate was also validated by MS 2 with the product anion of HSO 4 (96.9595; 0.82 ppm).…”

Section: Resultssupporting

confidence: 88%

“…The generation of ω-hydroxy-fatty acids by (CYP4A/F subfamilies) to generate the ω-hydroxy function and the acyltransferase(s) involved in the ultimate formation of the OAHFA products remain to be characterized in Meibomian glands. (O-acyl)-ω-Hydroxy-fatty acids have also been shown to serve complex lipid film roles in sperm and semen [15], and amniotic fluid [16], while cholesterol esters of (O-acyl)-ω-hydroxy-fatty acids predominate in the vernix caseosa of fetal skin [23].…”

Section: Discussionmentioning

confidence: 99%

“…With regard to the augmented OAHFAs, these are unlikely to originate from Moraxella bovis since they are a novel class of amphiphilic lipids that are only present in ocular fluids [12,13,14,18,19,20,21,22] and several other unique compartments [15,16,23].…”

Section: Discussionmentioning

confidence: 99%

“…The upper organic layer was isolated and dried by centrifugal vacuum evaporation and dissolved in isopropanol:methanol:chloroform (4:2:1) containing 7 mM ammonium acetate. The stable isotope internal standards included [ 2 H 5 ]PtdE 34:1, [ 2 H 5 ]DHA, [ 2 H 31 ]PtdC 34:1, [ 2 H 28 ]DCA 16:0, [ 2 H 7 ]cholesterol sulfate, and bromocriptine as internal standards [15,16,32].…”

Section: Methodsmentioning

confidence: 99%

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Tear Film Amphiphilic and Anti-Inflammatory Lipids in Bovine Pink Eye

et al. 2018

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Background: Tear film fluid serves as a dynamic barrier that both lubricates the eye and protects against allergens and infectious agents. However, a detailed analysis of a bacteria-induced immune response on the tear film lipidome has not been undertaken. Methods: We undertook a high-resolution mass spectrometry lipidomics analysis of endogenous anti-inflammatory and structural tear film lipids in bovine pink eye. Results: Bovine pink eye resulted in dramatic elevations in tear fluid levels of the anti-inflammatory lipids resolvin E2, cyclic phosphatidic acid 16:0, and cyclic phosphatidic acid 18:0. In addition, there were elevated levels of the structural lipids (O-acyl)-ω-hydroxy-fatty acids, cholesterol sulfate, ethanolamine plasmalogens, and sphingomyelins. Lipid peroxidation also was augmented in pink eye as evidenced by the hydroperoxy derivatives of ethanolamine plasmalogens. Conclusions: Ocular infections with Moraxella bovis result in the induction of a number of endogenous anti-inflammatory lipids and augmentation of the levels of structural glycerophospholipids and sphingolipids. Increased levels of hydroperoxy glycerophospholipids also indicate that this bacterial infection results in lipid peroxidation.

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Section: Resultssupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Tear Film Amphiphilic and Anti-Inflammatory Lipids in Bovine Pink Eye

et al. 2018

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“…Along with influencing the response to cooling and freezing, the lipid composition of sperm membranes plays a major role in the physiological changes leading to fertilization (Aksoy, Aksoy, Altinkaynak, Aydin, & Ozkan, 2006), and the low-temperature shock could cause irreversible damages on the sperm cell membrane. The reason some species are more susceptible than others is directly related to the lipid composition of the cellular membrane (Wood, Ball, Scoggin, Troedsson, & Squires, 2018).…”

Section: Introductionmentioning

confidence: 99%

Effect of feeding pomegranate seed oil as a source of conjugated linolenic acid on Arabian stallion semen quality in cooled and postthawed condition

Nouri

Shojaeian

Jalilvand

et al. 2018

Reprod Domestic Animals

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The objective was to assess the influence of pomegranate seed oil supplementation on the quality of fresh, cooled and frozen-thawed Arabian breed stallion semen. Eight stallions (n = 4 per group) received their normal diet (control group) or normal diet top dressed with 200 ml of pomegranate seed oil (PSO group). Semen was collected every fifteen days for 90 days. Stallions were reversed across the treatments after a sixty-day interval. In cooled and stored condition (2, 12 and 24 hr), spermatozoa motion characteristics, membrane integrity, viability, morphology and lipid peroxidation were analysed. In frozen-thawed semen, sperm dynamic characteristics were analysed by CASA, acrosome status and mitochondrial activity (evaluated by Flow cytometry) determined. The effects of treatment, time, semen type and their interactions were submitted to PROCMIX (SAS ), and means compared by the Tukey test. Also, collected semen samples were artificially inseminated to evaluate fertility and pregnancy rate after day 60 of the experiment. The results from fresh condition showed that semen volume, sperm concentration, abnormality and live sperm were not affected by dietary treatment (p > 0.05). In cooled condition, the higher value for sperm plasma membrane integrity and viability was observed in PSO group compared to control after 24 hr cooled and stored in 5°C. In postthawed condition, the higher value for CASA total motility and acrosome status was observed in PSO group compared to control group (p < 0.05). One hundred and twenty-six mares were artificially inseminated for fertility trial using control and PSO groups' fresh semen. The average pregnancy rates were not significantly different between control and treated group (62.88% and 65.90%, respectively) (p > 0.05). We concluded that under the conditions of this study, dietary supplementation of 200 ml pomegranate seed oil seems to relatively improved Arabian horse sperm quality during storage in cooled and frozen condition via increasing plasma membrane integrity, viability and acrosome status, but did not improve the pregnancy rates.

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Metabolomics of the amniotic fluid: Is it a feasible approach to evaluate the safety of Chinese medicine during pregnancy?

Shan

Xie

Zhou

et al. 2018

J of Applied Toxicology

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The use of Chinese medicines (CMs) during pregnancy has long been a major public health concern. Although CMs have been shown to be effective in treating infertility and preventing miscarriage, their use has been restricted, mainly because of limited knowledge of their potential toxicity. Accurate toxicology data are urgently required to assess whether these CMs are safe for maternal health and fetal development. Amniotic fluid (AF) contains carbohydrates, lipids and phospholipids, urea and proteins, all of which aid in the growth of the fetus and reflect the mother's health status as well. The changes in metabolomic patterns of AF are related to pathophysiological occurrences during the course of pregnancy. In this review, we provide a summary of the research performed in recent years on metabolomic AF samples, and use our previous study as an example to explore the feasibility of metabolomics of AF to evaluate the safety of CMs during pregnancy. We believe that metabolomics of AF play a far more important role than traditional morphology methods in the safety evaluation of CMs for pregnancy, with a higher sensitivity and correlation.

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Lipidomics of equine amniotic fluid: Identification of amphiphilic (O-acyl)-ω-hydroxy-fatty acids

Cited by 24 publications

References 29 publications

Tear Film Amphiphilic and Anti-Inflammatory Lipids in Bovine Pink Eye

Tear Film Amphiphilic and Anti-Inflammatory Lipids in Bovine Pink Eye

Effect of feeding pomegranate seed oil as a source of conjugated linolenic acid on Arabian stallion semen quality in cooled and postthawed condition

Metabolomics of the amniotic fluid: Is it a feasible approach to evaluate the safety of Chinese medicine during pregnancy?

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