Lipidomics Profiling of Skin Surface Lipids in Senile Pruritus

Abstract

Background: Senile pruritus is common, yet its etiology remains unknown. We examined the lipidomics profiles of skin surface lipids (SSL) in the elderly to better understand potential causes for senile pruritus. Methods:Transepidermal water loss (TEWL) was used to assess skin barrier function. Ameliorated Kawashima itch scale were used to measure the pruritus score. Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) and multivariate data analysis were used to investigate SSL alternations. Results:The results showed that the senile pruritus have higher TEWL values than controls (13.13±4.28 versus 6.71±2.45, p< 0.01). LC-MS/MS showed significant differences in lipidomics and identified 81 species of SSL that differ between two groups. Compared to controls, the levels of ceramides, diacylcerols, fatty acids, phosphatidylcholines, phosphatidylethar, phytosphingsines, sphingosines, diacylceryl-3-O-carboxyhydroxymethylcholine, diacylglyceryl trimethylhomoserine, unsaturated free fatty acids increased, whereas triacylglycerol decreased. CER-EOS, CER-NDS and CER-NS were positively correlated with TEWL values (p<0.05). Sphingomyelin, Cer-NP, Cer-AS, Cer-NDS, Cer-NS were positively correlated with pruritus severity scores, while Cer-BS, Cer-EODS, Cer-EOS, Cer-AP were negatively correlated. Conclusion:Our study indicated that the senile pruritus have impaired skin barrier function and altered SSL composition. Select SSL species identified in this study may be potential target for future studies on the pathogenesis of idiopathic senile pruritus

Effects of different plant growth regulators as the foliar application on growth and flower quality of potted rose (Rosa chinensis Jacq. cv. Nhung)

“…Several studies have shown that wilt, senescence and drop of petals were caused by an increasing activity by endogenous ethylene, deterioration of cell wall components and an increasing activity of destroyed enzymes (Van Doorn, 2002;Wagstaff et al, 2003;Lerslerwong et al, 2009). A decrease in endogenous plant growth regulators could enhance the process of wilt and senescence (Lukaszewska et al, 1994: Ma et al, 2005. Therefore, an exogenous supply of these regulators could improve quality and flowering time in several ornamental plants (Saffari et al, 2004;Hoeberichts et al, 2007;Jiang et al, 2010;Sardoei, 2014;Mondal and Sarkar, 2018).…”

Lipidomics profiling to reveal the restorative effect of moisturizer in OICD patients

“…Previous studies reported that the increased TEWL and decreased hydration content were associated with skin xerosis [12]. Skin surface lipids (SSLs) are a mixture of sebaceous gland lipids and intercellular lipids located in the outer layer of epidermal cells [13]. SSLs maintain skin barrier function, protect skin moisture content, and help regulate skin cell proliferation and apoptosis, cellular immunity, and in ammation.…”