Lipidomics Provides Novel Insights into Understanding the Bee Pollen Lipids Transepithelial Transport and Metabolism in Human Intestinal Cells

Abstract

Bee pollen (BP) shows profound gut-protecting potentials. BP lipids (BPLs) mainly composed by phospholipids and polyunsaturated fatty acids might be one of the important contributors, while how BPL exerts gut-protecting effects and is transported through intestinal cell monolayers need to be investigated. Here, we exploited a strategy that combines an UPLC-Q-exactive orbitrap/MS-based lipidomics approach with a human intestinal cell (Caco-2) monolayer transport model, to determine the transepithelial transportation of BPL from Camellia sinensis L. (BPL-Cs), in pathological conditions. The results showed that BPL-Cs protected Caco-2 cells against dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction by improving cell viability, maintaining membrane integrity, increasing tight junctions (ZO-1 and Claudin-1), and eliciting the expressions of antioxidative-related genes (NQO1, Nrf2, Txnrd1, and GSTA1). Lipidomics analysis revealed that DSS suppressed the transport and uptake of most of BPL-Cs including glycero­phospholipids, sphingo­myelins, and glycosyl­sphingolipids. Pretreatment with BPL-Cs significantly regulated glycerophospholipid and sphingolipid metabolisms, potentially involved in building permeability barriers and alleviating intestinal oxidative stress. Finally, eight classes of lipids were identified as the potential biomarkers for evaluating DSS-induced Caco-2 cell dysfunctions and BPL-intervened modulation. These findings shed light on the development of BPL as gastrointestinal protective food supplements in the future.

Photoprotective Mechanism of Fucoxanthin in Ultraviolet B Irradiation-Induced Retinal Müller Cells Based on Lipidomics Analysis

“…Similarly, glycerophospholipid and sphingolipid metabolism regulation was explored in dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction Caco-2 cells. 15 Sun et al suggested that the antioxidative mechanism of geissoschizine methyl ether in glutamate-induced HT-22 cells is mediated via lipid metabolism regulation. 16 However, few studies have focused on the photoprotective effects of FX in RMCs, and the protective mechanism of FX against UVB irradiation in the retina is not well-understood.…”

“…Vats et al found that ferroptosis induced by intracellular cellular inflammation due to UVB is related to excessive phospholipid peroxidation. Similarly, glycerophospholipid and sphingolipid metabolism regulation was explored in dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction Caco-2 cells . Sun et al suggested that the antioxidative mechanism of geissoschizine methyl ether in glutamate-induced HT-22 cells is mediated via lipid metabolism regulation …”

Comprehensive Lipidomics Analysis Reveals the Effects of Different Omega-3 Polyunsaturated Fatty Acid-Rich Diets on Egg Yolk Lipids

“…To address this problem, more powerful techniques are required, and mass spectrometry (MS) exerts overwhelming potentials in lipid structure elucidation. In recent years, high-resolution MS, such as quadrupole time-of-flight MS (Q-TOF-MS) and Orbitrap, has been developed and applied in untargeted lipid analysis of different food matrices including fish, meat, and milk. − When combined with highly selective ultraperformance liquid chromatography (UPLC), this advanced analytical technique enables the identification of hundreds of lipids in an unbiased way and has demonstrated its feasibility and potential in food science research at the molecular level. − In terms of egg yolk lipids, previous studies using MS-based lipidomics approaches were mainly focused on a certain class or several lipid classes from common or various types of eggs. − For example, Ali and co-workers identified 57 PL molecules in duck, hen, and quail egg yolk samples . To the best of our knowledge, there are limited studies that systematically investigate the lipidome changes in eggs after dietary manipulation.…”

A Novel Mast Cell Stabilizer JM25-1 Rehabilitates Impaired Gut Barrier by Targeting the Corticotropin-Releasing Hormone Receptors

“…The JM25-1 alone has no significant impact on ZO-1, which is shown in Figure S1 . Additionally, transepithelial electrical resistance (TEER) of Caco2 cell monolayers is a common model used to investigate the permeability of the intestinal barrier [ 23 ]. The TEER value continuously dropped due to the co-culture with the medium of HMC-1 cells pre-treated with CRH.…”