Lipids accumulation in Rhodotorula glutinis and Cryptococcus curvatus growing on distillery wastewater as culture medium

Abstract

Oleaginous yeasts R. glutinis and C. curvatus were grown in batch culture on high strength distillery wastewaters from the Tequila production process (Tequila's vinasses). Both strains were able to grow either on decolorized charcoal or on naturally colored regular vinasses, with no extra nutrients addition. After 144 h of culture, lipids were accumulated up to 25.2 ± 1.98 and 27.02 ± 2.36% of cell dry weight, in C. curvatus and R. glutinis, respectively. At the same time, the chemical oxygen demand of vinasses (23,125 ± 5,557) was removed by C. curvatus and R. glutinis up to 78.98 ± 1.38 and 84.44 ± 3.52%, respectively. The fatty acid profile of the lipids accumulated by both strains was composed by myristic, palmitic, stearic, oleic, and linoleic. Linolenic acid was also found but only in the intracellular lipids of R. glutinis. This is the first report of the utilization of Tequila's vinasses for the production of microbial oil by oleaginous yeast. This process could become a strategy to transform this highly contaminant wastewater into value added lipids. © 2011 American Institute of Chemical Engineers Environ Prog, 32: 69–74, 2013.

Integrated Process for the Enzymatic Production of Fatty Acid Sugar Esters Completely Based on Lignocellulosic Substrates

“…Under optimized conditions, C. curvatus accumulates up to 60% lipid per dry biomass (Ratledge, 1991 ). Previous studies showed that C. curvatus can utilize waste streams from industry, e.g., distillery or brewery waste, to produce SCO (Gonzales-Garcia et al, 2013 ; Ryu et al, 2013 ). Additionally, some hydrolysates, such as dilute sulfuric acid hydrolysates of wheat straw (with emphasis on acetate utilization) (Yu et al, 2014 ) and alkaline-pretreated corn stover hydrolysate (resulting in ~30% (w/w) lipid content) (Gong et al, 2015 ) have been used as a substrate.…”

Sugarcane Distillery Spent Wash, a New Resource for Third-Generation Biodiesel Production

“…Gonzalez-Garcia et al [51] reported that Cryptococcus curvatus and Rhodotorula glutinis produced respectively 5.19 and 6.06 g/L of biomass on pure distillery wastewater. Using our local vinasse, we obtained biomass production yields of 4.54 g/L, 6.4 g/L, 7.64 g/L, and 7.8 g/L, respectively for Cryptococcus albidus, R. glutinis, R. graminis, and R. mucilaginosa which were in good agreement with reported data from Gonzalez-Garcia et al [51].…”

“…Gonzalez-Garcia et al [51] reported that Cryptococcus curvatus and Rhodotorula glutinis produced respectively 5.19 and 6.06 g/L of biomass on pure distillery wastewater. Using our local vinasse, we obtained biomass production yields of 4.54 g/L, 6.4 g/L, 7.64 g/L, and 7.8 g/L, respectively for Cryptococcus albidus, R. glutinis, R. graminis, and R. mucilaginosa which were in good agreement with reported data from Gonzalez-Garcia et al [51]. However, they obtained lipid production values of 1.49 g/L and 1.86 g/L, respectively for C. curvatus and R. glutinis after 144 h of culture on pure vinasse, whereas we found that the best lipid production from yeasts was 0.98 g/L for C. curvata after 240 h of culture.…”

Draft Genome Sequence of the Oleaginous Yeast Cryptococcus curvatus ATCC 20509

“…Cryptococcus curvatus ATCC 20509 is an oleaginous yeast strain capable of assimilating xylose, lactose, glucose, and sucrose, as well as a variety of agricultural and food-processing wastes, as carbon sources (1–3). Lipid production in C. curvatus ATCC 20509 can be induced under nitrogen-limiting conditions (4) and favors the synthesis of 18-carbon-chain-length fatty acids (5).…”