Lipids and fatty acids in wild and pond-reared mud crab Scylla serrata (Forsskål) during ovarian maturation and spawning

Alava, Veronica R.; Quinitio, Emilia T.; Pedro, Jennete B De; Priolo, Flora Mae P.; Orozco, Zenith Gaye A.; Wille, Mathieu

doi:10.1111/j.1365-2109.2007.01793.x

Cited by 55 publications

(42 citation statements)

References 34 publications

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“…As a corollary to the external and histological classification, the biochemical profiles of the same batch of ovaries were determined separately (Alava, Quinitio, de Pedro, Priolo, Orozco & Wille 2007). The characteristics of the quality and quantity of egg yolk proteins incorporated into the eggs may have a direct and measurable effect on larval survival.…”

Section: Discussionmentioning

confidence: 99%

Ovarian maturation stages of the mud crab Scylla serrata

2007

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Ovarian maturation in adult wild‐sourced and pond‐grown Scylla serrata (Forsskål) was determined based on gross morphology and histological appearance. There were no significant differences noted in the histological features of both wild and pond‐reared S. serrata females. Ovarian maturation was classified into five stages: immature, early maturing, late maturing, fully mature and spent. The immature ovaries are thin and translucent to off white and contain oogonia, primary oocytes with large nuclei. The follicle cells were found around the periphery of the lobes and an area among groups of oogonia and oocytes. The follicle cells gradually enclosed the oocytes. The early‐maturing ovaries were yellow and small yolk globules started to appear in larger oocytes. In late‐maturing ovaries, the colour became light orange and lobules were apparent. Yolk globules occurred in the cytoplasm with larger globular inclusions towards the periphery, while follicle cells were hardly recognizable. Fully mature ovaries were orange to deep orange and had swollen lobules. Large yolk globules were apparent in the entire cytoplasm. Follicle cells were hardly seen. Spent ovaries were similar to the early‐maturing and late‐maturing stage in partially spawned females. The ovarian development was correlated closely to the gonadosomatic index, oocyte diameter, and ovarian histology. The classification of ovarian maturation provides baseline information for further studies on reproductive biology. Likewise, the information provides a guide for broodstock management in the hatchery.

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Section: Discussionmentioning

confidence: 99%

Ovarian maturation stages of the mud crab Scylla serrata

2007

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“…It is generally accepted that optimizing feed formulation specifically for broodstock should enhance egg quality, gonad maturation, and fecundity in crustaceans (Rodríguez‐González et al 2009), and therefore reflect their nutrient requirements and metabolic needs (Alava et al 2007). Prior experiments conducted on red claw in our laboratory indicated that dietary EPA and DHA were inefficiently utilized during vitellogenesis, suggesting that a costly diet with high PUFA content may not meet nutritional demands.…”

Section: Introductionmentioning

confidence: 99%

Effect of different dietary lipid sources on growth and gonad maturation of pre-adult female Cherax quadricarinatus (von Martens)

Guo

Gan

et al. 2011

Aquaculture Nutrition

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The effects of four dietary lipid sources (fish oil, peanut oil, soybean oil, pork lard) on growth and gonad maturation of pre‐adult female red claw crayfish, Cherax quadricarinatus, were evaluated. Performance was evaluated by weight gain, specific growth rate (SGR), survival, feed conversion ratio (FCR), gonadosomatic index (GSI), fatty acid composition, and vitellogenin (vg) gene expression. The proportion of saturated fatty acids (SFA), mono‐unsaturated fatty acids (MUFA), and poly‐unsaturated fatty acids (PUFA) in hepatopancreas varied as a result of lipid source, while differences in muscle were limited to MUFA levels. Vg expression and growth, but not GSI, differed significantly, with peak expression and optimal growth observed in red claws receiving the soybean oil diet. Conversely, diets high in EPA (20:5n3) and DHA (22:6n3) produced suboptimal results, suggesting that the importance of maturation diets with high EPA and DHA content may be exaggerated. As the dietary lipid requirements of growing and reproductively active red claw crayfish were satisfied by a plant oil that contained high levels of 18‐carbon unsaturated fatty acids, soybean oil may be an economic alternative to the expensive lipid‐fortified feed currently utilized by the aquaculture industry.

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“…Lipids are one of the main sources of metabolic energy and have important functions as cytoplasm and membrane constituents of cells affecting structural and physiological properties. Wild and pond‐sourced crab S. serrata during ovarian maturation and spawning indicated that the ovaries and newly spawned eggs contain high levels of highly unsaturated fatty acids (HUFA) such as arachidonic acid (ArA, 20:4n‐6), eicosapentaenoic acid (EPA, 20:5n‐3) and docosahexaenoic acid (DHA, 22:6n‐3) (Alava, Quinitio, de Pedro, Orosco, Priolo & Wille 2007). Moreover, phospholipids particularly phosphatidyl choline (PC) were also accumulated in large amounts in ovaries and newly spawned eggs.…”

Section: Introductionmentioning

confidence: 99%

Reproductive performance, lipids and fatty acids of mud crab Scylla serrata (Forsskål) fed dietary lipid levels

et al. 2007

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Natural food (NF, control), artificial diets (AD) containing total lipid levels of 10%, 12% and 14% (AD10, AD12 and AD14) and their combinations (AD10+NF, AD12+NF and AD14+NF) were fed for 112 days to pond‐sourced eyestalk‐ablated mud crab Scylla serrata (625±6.4 g) in tanks in order to determine their effects on reproduction and lipid profiles in broodstock tissues and zoeae. Crabs fed NF had the highest number of spawning followed by crabs fed AD10+NF and AD14+NF. Higher offspring production (number of zoeae) was obtained from crabs fed NF and AD+NF than from AD. As dietary total lipid levels increased, total lipid of broodstock ovaries, hepatopancreas, muscle and zoeae correspondingly increased in which AD+NF promoted higher levels than AD. Increased dietary total lipid levels enhanced lipid classes such as triacylglycerols and phosphatidyl choline levels in zoeae, all higher in crabs fed AD+NF than in AD. The major fatty acids in zoeae, particularly 16:0, 18:0, 18:1n‐9 and 20:4n‐6, 20:5n‐3 and 22:6n‐3, were higher in crabs fed AD+NF than in AD, the contents corresponding to broodstock dietary total lipid levels. A 10% total lipid in AD in combination with NF was sufficient to provide the essential lipids in crabs in the improvement of larval production and quality.

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Lipids and fatty acids in wild and pond-reared mud crab Scylla serrata (Forsskål) during ovarian maturation and spawning

Cited by 55 publications

References 34 publications

Ovarian maturation stages of the mud crab Scylla serrata

Ovarian maturation stages of the mud crab Scylla serrata

Effect of different dietary lipid sources on growth and gonad maturation of pre-adult female Cherax quadricarinatus (von Martens)

Reproductive performance, lipids and fatty acids of mud crab Scylla serrata (Forsskål) fed dietary lipid levels

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