Lipids as endogenous Na, K-ATPase inhibitors in plasma of healthy individuals and in dialysis dependent patients

Vasdev, Sudesh; Longerich, L; Prabhakaran, V.; Triggle, Chris R.; Gault, M. H.

doi:10.1016/s0009-9120(89)80025-6

Cited by 11 publications

(9 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The marked rise in nonesterified fatty acids (N EFA) during hemodialysis with heparin has been known for more than 20 years [1], and we have observed a 3-to 15-fold increase [2]. N E F A were found to rise more when heparin was given during hemodialysis than when it was given without hemodialysis, and the difference was considered to be due to as yet unknown factors [3,4], N E F A such as oleic reports lend some support to the proposal that the rise in N E F A during hemodialysis with heparin and an infusion of a triglyceride emulsion led to fatal arrhythmias in 2 patients [9] and that such treatment in patients with ischemic hearts could be dangerous because o f the potential myocardial toxicity o f high N E F A levels [7,[10][11][12][13].…”

Section: Introductionmentioning

confidence: 51%

“…Oleic and linoleic acids are the principal N E F A which we found to increase 3H-ouabain displacement from N a,KATPase in vitro [2], but only linoleic acid was found to appreciably alter 86Rb uptake (table 5). In our patients, linoleic acid showed the greatest proportional increase in concentration during dialysis with heparin (table 1) and had the highest correlation with both ' H-ouabain displacement and 86Rb uptake tests (table 4).…”

Section: Changes In Individual Nefa During Dialysis With Heparinmentioning

confidence: 59%

“…Similarly, neither diphosphatidylglycerol nor DL-palmitoylcamitine would have influenced the 3H-ouabain displacement assay. However, lysophosphatidyl choline, whose concentration can increase with heparin, may affect ' H-ouabain displacement even at plasma levels found without heparin [2].…”

Section: Dialysis Without Heparinmentioning

confidence: 96%

See 2 more Smart Citations

Heparin, Fatty Acids and Sodium, Potassium-ATPase Inhibition by Plasma Factors during Hemodialysis

Gault¹,

Vasdev²,

Longerich³

et al. 1992

Nephron

Self Cite

View full text Add to dashboard Cite

To assess the relationship between heparin and the associated increase in nonesterified fatty acids (NEFA) and their possible influence on NaK-ATPase during hemodialysis, we studied two groups of patients: (1) 12 patients on chronic hemodialysis dialysed with heparin and (2) 6 patients dialysed without heparin. All 12 patients who received heparin anticoagulation had a 7-fold rise in NEFA on average and also had an increase in circulating inhibitors of NaK-ATPase assayed by ³H-ouabain displacement from NaK-ATPase and/or by effect of plasma on the uptake of ⁸⁶Rb by rat aortic rings. Serial assays in 3 of the patients receiving heparin showed NEFA and inhibitory changes to be at or near maximum within 30-60 min. Of the individual NEFA, the greatest relative increases were in oleic (18:1) and linoleic (18:2) acids, and the strongest correlations were between linoleic acid and both ³H-ouabain displacement (r = 0.94) and ⁸⁶Rb uptake (r=0.86). However, a small and slower increase in NEFA also occurred in 3 of the patients dialysed without heparin. We conclude that heparin anticoagulation during dialysis leads to a rapid and marked increase in circulating NEFA, particularly the unsaturated fatty acids, with a corresponding interference with NaK-ATPase activity. The clinical significance of these findings is unknown. The rise in NEFA during dialysis without heparin in some patients suggests that factors other than heparin may also contribute to the rise in NEFA.

show abstract

Section: Introductionmentioning

confidence: 51%

Section: Changes In Individual Nefa During Dialysis With Heparinmentioning

confidence: 59%

See 1 more Smart Citation

Heparin, Fatty Acids and Sodium, Potassium-ATPase Inhibition by Plasma Factors during Hemodialysis

Gault¹,

Vasdev²,

Longerich³

et al. 1992

Nephron

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, these 'cross-reactivities' in the digoxin RIA could not be demonstrated when physiolo gic levels of DHEA, DHEA-S, fatty acids, or cortisol were as sayed [28,45]. Additionally, during an ITT, fatty acids decrease acutely and normalize after 2 h [21], DHEA-S does not change acutely [33], DHEA rises but is at negligibly low levels [33], and the rise in cortisol appears to precede slightly the rise in DLF [ I ].…”

Section: Discussionmentioning

confidence: 99%

Increases in Plasma Digitalis-Like Factor Activity during Insulin-Induced Hypoglycemia

et al. 1989

View full text Add to dashboard Cite

Digitalis-like factor (DLF) appears to be widely distributed throughout the body, and has been specifically localized to the mammalian hypothalamus. In this study we monitored the DLF response to insulin-induced hypoglycemia in 11 normal males. Hypoglycemia was associated with a significant increase in serum DLF activity over basal values (40 ± 5 pg digoxin Eq/ml, mean ± SEM) at 60 min (83 ± 8 pg digoxin Eq/ml) and 90 min (86 ± 10 pg digoxin Eq/ml) following insulin administration (p < 0.01). We measured the DLF response to insulin-induced hypoglycemia in other men after infusion of hypertonic saline (n = 8) or after oral water-loading (n = 8). Saline infusion (0.06 ml 5% saline/kg BW/min for 120 min prior to insulin infusion) or water loading (20 ml/kg BW over 60 min completed 30 min before insulin infusion) alone significantly increased serum DLF (saline: 21 ± 5 to 60 ± 18 pg digoxin Eq/ml, p < 0.05; water: 18 ± 11 to 49 ± 15 digoxin Eq/ml, p < 0.05). DLF activity was further significantly increased within each group 60 and 90 min after administration of insulin. In the group infused with hypertonic saline, insulin caused increases of serum DLF from 60 ± 18 at time 0 to 170 ± 17 pg digoxin Eq/ml at peak (p < 0.01). Similarly, in the oral water-loaded group serum DLF increased from 49 ± 15 at time 0 to 127 ± 24 pg digoxin Eq/ml at peak (p < 0.01) after they received insulin. These results suggest that DLF may be involved in the acute stress response to insulin-induced hypoglycemia, and this response may be augmented by salt or fluid loading.

show abstract

“…Maintaining the daily sodium balance is the main feature of this regulation because sodium influences both the extracellular volume and the distribution of water between intracellular and extracellular compartments. During the development of chronic renal failure, the ability in maintaining sodium balance becomes increasingly impaired and directly results in changes of the extracellular fluid [1]. The extreme situation is exemplified by end-stage renal disease (ESRD) patients treated by hemodialysis who suffer from intermittent fluctuations in hydration status and plasma electrolyte concentrations.…”

Section: Introductionmentioning

confidence: 99%

Sequential body composition analysis by bioimpedance early post-kidney transplantation

et al. 2005

View full text Add to dashboard Cite

Summary Background: While chronic renal failure patients present disturbed body water composition, few studies have been done on its behavior following kidney grafting (Tx). We report the changes associated with a successful Tx on body composition evaluated by bioelectrical impedance analysis (BIA). Methods: Twelve Tx (seven males, five females) were studied. The BIA was assessed before Tx, at month 1 and at month 3 post‐Tx. Total body water (TBW), extracellular water (ECW), intracellular water (ICW), Na:K exchange rate (Nae:Ke) and phase angle (PA) were studied. An healthy group and a HD group were evaluated three times in a year interval. Results: Comparing before Tx with month 1 post‐Tx, TBW, ECW and Nae:Ke increased, while ICW and PA decreased significantly. Comparing month 1 with month 3 post‐Tx, ECW decreased, while ICW and PA increased. On comparing month 1 post‐Tx with the healthy group, Nae:Ke was greater and PA was lower at month 1. Conclusions: The BIA showed that the different body water compartments of Tx recipients quickly match the constitution of normal individuals, overcoming drug therapy side effects.

show abstract

Lipids as endogenous Na, K-ATPase inhibitors in plasma of healthy individuals and in dialysis dependent patients

Cited by 11 publications

References 26 publications

Heparin, Fatty Acids and Sodium, Potassium-ATPase Inhibition by Plasma Factors during Hemodialysis

Heparin, Fatty Acids and Sodium, Potassium-ATPase Inhibition by Plasma Factors during Hemodialysis

Increases in Plasma Digitalis-Like Factor Activity during Insulin-Induced Hypoglycemia

Sequential body composition analysis by bioimpedance early post-kidney transplantation

Contact Info

Product

Resources

About