Lipids in the skin of a cat-fish Heteropneustes fossilis (Bloch) (Heteropneustidae, Pisces)

Mittal, Ajay Kumar; K., A.; Banerjee, Tanmoyee; Agarwal, S. K.

doi:10.1007/bf00497453

Cited by 11 publications

(10 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This substance apparently has the property to form a gel in the presence of water, and it is probably emitted from the wounded tissues. This phenomenon is similar to that described after a teleost skin incision by Mittal et al (1978).…”

Section: Discussionsupporting

confidence: 91%

“…This substance allows the cells to spread as much as those spreading on the SPL surface. The properties of the exudate in favouring cell spreading have already been reported by Mittal et al (1978).…”

Section: Discussionsupporting

confidence: 60%

“…During skin wound healing in mammals, the epidermal cells have to move beneath an exudate forming the scab to avoid drying out, and consequently the re‐epithelialization process is slow (7 to 20 μm/hr, Vanable, 1989). In actinopterygians, as in amphibians, the aquatic environment no doubt facilitates the epidermal migration over the wound because of the presence of a more appropriate moist substrate (Mittal et al, 1978). This hypothesis is supported by the fact that migration of the epidermis is faster in mammals in moist environment (cornea, gingiva, trachea; Ubels et al, 1982; Keenan et al, 1983; Wunsh and Ide, 1992).…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, studies of wound healing in teleost are scarce. They either concern wound closure after small incisions of the skin and the defence reactions against pathogens (Phromsuthirak, 1977; Mittal et al, 1978) or they focus on the substrate on which epidermal cells migrate (Sire et al, 1990; Kurokawa et al, 1993). To our knowledge no study has been devoted so far to the healing of large superficial wounds after scale and epidermis removal in teleosts.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Spreading, proliferation, and differentiation of the epidermis after wounding a Cichlid fish,Hemichromis bimaculatus

Quilhac

Sire

1999

Anat. Rec.

View full text Add to dashboard Cite

A large superficial wound has been experimentally provoked in the cichlid fish Hemichromis bimaculatus to study the interactions between the epidermal cells and the substrate on which they spread, on the one hand, and the restoration of the subepidermal tissues and the epithelial-mesenchymal interactions preceding scale regeneration, on the other hand. The re-epithelialization process, e.g., migration, spreading, differentiation, and proliferation of the epidermal cells, has been followed step by step, using light, scanning and transmission electron microscopy, and tritiated thymidine incorporation, until complete reorganization of the healing epidermis. Wound healing is fast (500 microm/hr) and proceeds centripetally from the wound margins. The epidermal cells spread on a wound surface which is composed of two different matrices: the remains of basement membrane materials covering the scale-pockets, and collagen fibrils of cut dermal strips. Even though both matrices favour cell spreading and attachment, migrating cells show a different behaviour. The re-epithelialization of the wound follows an orderly sequence similar to amphibian and mammalian wound healing, i.e., a "leap frog" mechanism of cell locomotion involving three epidermal layers. The basal layer cells, which spread on the substrate, and the superficial layer cells which protect the epidermis, differentiate first. Whatever the type of substrate over which the epithelium spreads (basement membrane material or collagen fibrils), the epidermal basal layer cells differentiate as soon as they become attached. The incorporation of tritiated thymidine has revealed that there is no proliferation in the healing epidermis until after complete closure of the wound, but that the rapid re-epithelialization of the large surface requires the recruitment of epidermal cells at the wound margins. The present study offers new data on the dynamics of re-epithelialisation and on the resistance of cichlid skin to such wounds. It is also clearly shown that the epidermal basal layer cells differentiate rapidly, a step which is interpreted as the first stage of epithelial-mesenchymal interactions that will lead to scale regeneration.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Spreading, proliferation, and differentiation of the epidermis after wounding a Cichlid fish,Hemichromis bimaculatus

Quilhac

Sire

1999

Anat. Rec.

View full text Add to dashboard Cite

show abstract

“…Therefore, in different organisms including fish, healing of skin wounds is of utmost importance for their survival. Histopathological studies, using light microscope, on healing of skin wounds in different fish species are reported by several workers- Mittal and Munshi (1974) in Rita rita; Anderson and Roberts (1975) in Tanichthyes albonubes and Salmo salar; Mittal, Rai, and Banerjee (1978) in Heteropneustes fossilis; Banerjee and Mittal (1999) in Channa striata; Silva et al (2005) in Notothenia coriiceps; Bader, Nusbaum, and Shoemaker (2003) in Ictalurus punctatus; Guerra, Santos, Cecarelli, Silva, and Hernandez-Blazquez, (2008) in Clarias gariepinus and Richardson et al (2013) in Danio rerio.…”

Section: Introductionmentioning

confidence: 95%

Scanning electron microscope investigation on the process of healing of skin wounds in Cirrhinus mrigala

Verma

Kumārī

Mittal

et al. 2017

Microscopy Res & Technique

View full text Add to dashboard Cite

Present scanning electron microscope study, reports healing of excised skin wounds in Cirrhinus mrigala. Healing process of wounds, inflicted on head skin, using biopsy punch was observed at intervals-0 hour (h), 1, 2, 4, 6, 12 h, 1 day (d) 2 and 4 d. Accumulation of mucus in wound region within 1h after infliction of wound has been considered an immediate measure to provide protection to injured skin from microbial invasion and other external environmental hazards. On infliction of wound, mobilization of epithelial cells at wound edge is associated with disturbance of coaptive relationship of epithelial cells till original coaptive stability is reached. At 6-12 h appearance of epidermal ridge in region of contact of migrating fronts is due to piling up of epithelial cells. This is associated with cessation of migration of epithelial cells and their simultaneous continual arrival in the region. Speedy epithelialization of skin wounds in C. mrigala like in other fishes, compared to that of mammals and other higher vertebrates, is possibly facilitated owing to surrounding wet external environment. Microridges in initial stages of wound healing appear fragmented without particular orientation. Further, epithelial cells in epithelium in wound region and in region adjacent to wound elongate. These changes are associated with the stretching of epithelial cells indicating their streaming and migration, toward wound. Presence of superficial neuromasts, smallest functional units of lateral line system, a hydrodynamic sensory system, has been associated with important functional significance in fish.

show abstract

Healing of cutaneous wounds in a freshwater teleost, Labeo rohita: Scanning electron microscopical investigation

Kumari¹,

Srivastava²,

Nigam³

et al. 2012

Microscopy Res & Technique

View full text Add to dashboard Cite

In this study, healing of cutaneous wounds in Labeo rohita using scanning electron microscope is reported. Wound area could be divided into three regions. Immediately after infliction of wound, edges retract exposing underlying tissues in wound gap (Region I). Simultaneously, at region close to wound edge (Region II), mucous goblet cell openings are observed with copious mucous secretions. Within 1 h, Region I gets covered by mucous secretions, and epidermis at edges starts migrating. Opposing fronts gradually advance and by 4-6 h come in contact to epithelialize wound gap. Zone of contact of fronts is demarcated by epidermal ridge, which is relatively prominent at 8 h. It gradually diminishes and is not distinguished at 24 h and afterward. At 1-4 h, microridges on epithelial cell surfaces appear irregularly arranged, widely spaced, short with abrupt ends at Region I; relatively extensive at Region II; and similar to those in controls at region surrounding Region II (Region III). At 12 h and afterward, microridges appear similar to those in controls at Regions I and II. At 1-2 h, isolated swollen epithelial cells, often in process of detachment and exfoliation at surface, are observed at Regions I and II. Such cells are infrequent at 8 h and afterward. Region I covered by migrated epidermis appears trough like at 4 h to 2 days, level of which gradually rises and at Day 4, surface of epidermis appears at a level similar to that at Regions II and III. Changes have been associated with the imbalance of osmotic homeostasis due to disruption of barrier between internal and external environment of skin.

show abstract

Lipids in the skin of a cat-fish Heteropneustes fossilis (Bloch) (Heteropneustidae, Pisces)

Cited by 11 publications

References 15 publications

Spreading, proliferation, and differentiation of the epidermis after wounding a Cichlid fish,Hemichromis bimaculatus

Spreading, proliferation, and differentiation of the epidermis after wounding a Cichlid fish,Hemichromis bimaculatus

Scanning electron microscope investigation on the process of healing of skin wounds in Cirrhinus mrigala

Healing of cutaneous wounds in a freshwater teleost, Labeo rohita: Scanning electron microscopical investigation

Contact Info

Product

Resources

About