Lipoamino Acid Coated Superparamagnetic Iron Oxide Nanoparticles Concentration and Time Dependently Enhanced Growth of Human Hepatocarcinoma Cell Line (Hep‐G2)

Abstract

Superparamagnetic iron oxide nanoparticles (SPION) have been widely used in medicine for magnetic resonance imaging, hyperthermia, and drug delivery applications. The effect of SPION on animal cells has been a controversial issue on which there are many contradictions. This study focused on preparation of SPION with novel biocompatible coatings, their characterization, and cytotoxicity evaluation. An amino acid (glycine) and two novel lipo-amino acids (2 amino-hexanoic acid and 2 amino-hexadecanoic acid) coated magnetic nanoparticles were characterized by various physicochemical means such as X-ray diffraction (XRD), transmission electron microscopy (TEM), vibrating sample magnetometry (VSM), differential scanning calorimetry (DSC), and infrared spectroscopy (FT-IR). The cytotoxicity profile of the synthesized nanoparticles on Hep-G2 cells as measured by MTT assay showed the nanoparticles are nontoxic and the cell growth is promoted by SPION. Moreover, lipoamino acid coating SPION appear more beneficial than the other ones. By increasing concentration of SPION, growth enhancing impact will attenuate and toxicity will appear. Although the aggregation of SPION can affect the results, the gradual delivery of ferric/ferrous ions into cells is the main cause of this growth promotion effect. Conclusively, this study shows that lipoamino acid coating SPION can be used for various biomedical purposes.

Green Synthesis of Silver Nanoparticles using Mediterranean Cypress (<i>Cupressus sempervirens</i>) Leaf Extract

“…X-ray powder diffraction (XRD, Siemens D5000) analysis was done to determine the crystallinity of prepared AgNPs. Functional groups of the phytochemicals which coat the prepared nanoparticles were determined by Fourier transformed infrared spectroscopy (Bruker, Vertex 70, FTIR Spectrometer) (Ebrahiminezhad et al, 2013;Gholami et al, 2015).…”

Magnetic immobilization of Bacillus subtilis natto cells for menaquinone-7 fermentation

“…Many of the previous studies revealed that IONs can interrupt the integrity of cell membrane and adversely affect normal cell functionalities (Berry et al 2004;Liu et al 2004;Can et al 2009). Contrary, some studies have reported that IONs at certain concentrations show no negative toxic or inhibitory effects on some microbial cells and also there are growth stimulatory effects (Grumezescu et al 2010;Ficai et al 2012;Islam et al 2012;Gholami et al 2015). By reviewing the literature, it seems that the effect of IONs on the growth and metabolic profile of microorganisms depends on the cell type.…”

Physicochemical and biological characteristics of the nanostructured polysaccharide-iron hydrogel produced by microorganismKlebsiella oxytoca

“…. FeO characteristic peak of Fe‐EPS appeared at 555 cm −1 which indicates the presence of iron oxide‐hydroxide nanoparticles . The CO and CC stretching vibration peaks were observed at 1027 and 1403 cm −1 respectively.…”