Lipocalin-like Prostaglandin D Synthase (L-PGDS) Concentration in Aqueous Humour in Patients With Open-angle Glaucoma

Abstract

PURPOSE:: To report on the concentration of lipocalin-like prostaglandin D synthase (L-PGDS) in the aqueous humour (AH) in patients with open-angle glaucoma (OAG). PATIENTS AND METHODS:: Prospective assessment in 20 patients (13 female, 7 male, mean age 74±10.6 y) who underwent surgery for OAG. AH was sampled and analyzed for L-PGDS concentration. AH from 26 patients (11 female, 15 male, 72.4±14.4 y) without glaucoma who underwent cataract surgery, served as control subjects. RESULTS:: The L-PGDS concentration in the AH sampled from the anterior chamber in the OAG group (5.9±2.4 mg/L) was significantly (P<0.001) higher than in the control group (3.3±1.3 mg/L). There were no significant differences between the concentrations of L-PGDS between the left and the right eye or between genders. CONCLUSIONS:: L-PGDS concentration in the AH of patients with OAG was significantly elevated compared with its concentration in the AH of nonglaucomatous eyes. As L-PGDS is a biologically pluripotent protein, its possible role in glaucoma warrants further examination. Patients and Methods: Prospective assessment in 20 patients (13 female, 7 male, mean age 74 ± 10.6 y) who underwent surgery for OAG. AH was sampled and analyzed for L-PGDS concentration. AH from 26 patients (11 female, 15 male, 72.4 ± 14.4 y) without glaucoma who underwent cataract surgery, served as control subjects.Results: The L-PGDS concentration in the AH sampled from the anterior chamber in the OAG group (5.9 ± 2.4 mg/L) was significantly (P < 0.001) higher than in the control group (3.3 ± 1.3 mg/L). There were no significant differences between the concentrations of L-PGDS between the left and the right eye or between genders.Conclusions: L-PGDS concentration in the AH of patients with OAG was significantly elevated compared with its concentration in the AH of nonglaucomatous eyes. As L-PGDS is a biologically pluripotent protein, its possible role in glaucoma warrants further examination.

Literature review and meta-analysis of translaminar pressure difference in open-angle glaucoma

“…Twenty-five papers were excluded because there was no evaluation of TPD. 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 Three papers were eliminated because glaucoma patients were not analyzed in these studies. 59 , 60 , 61 , 62 Three articles which did not present quantitative TPD values in OAG group were also excluded 62 , 63 , 64 Table 1 shows the characteristics of papers (including 181 POAG patients and 215 healthy subjects) included to systematic review.…”

Normal tension glaucoma: review of current understanding and mechanisms of the pathogenesis

“…In addition, a large concentration gradient of betatrace protein was found between the lumbar CSF and the CSF in the orbital SAS of the optic nerve [86]. Interestingly, a difference in protein content was also found in the anterior chamber of glaucoma patients compared to a non-glaucoma group [87,88]. In an experimental study [89] in sheep, the optic nerve was slightly ligated close to the optic canal to create an optic nerve sheath compartment.…”

What is the optimal glaucoma treatment: reducing aqueous humour production or facilitating its outflow?

“…In the case of glaucoma an abnormal accumulation of potentially toxic proteins was found not only in the subarachnoid space of the optic nerve but also in the aqueous humour (AH) of the anterior chamber. The protein content measured in the AH of glaucoma versus non glaucoma patients has demonstrated significant differences [ 18 , 19 ].…”

“…Interestingly a study comparing the concentration of betatrace protein in AH of cataract and glaucoma patients demonstrated a larger concentration of betatrace in the AH in the glaucoma group [ 18 ]. The role of betatrace protein is manyfold as it is a prostaglandin synthetase.…”