Lipocortins and phospholipase A2 regulation

García, María Teresa Espinosa; Zipfel, M; Buhl, W J

doi:10.1042/bst0181231

Cited by 3 publications

(1 citation statement)

References 2 publications

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“…572 Later two species of PLA 2 were partially purified and characterized from human placental membranes. 573 Molecular cloning and sequence analysis of cDNA clones encoding PLA 2 from human placenta were described. 574 Critical Reviews in Clinical Laboratory Sciences Downloaded from informahealthcare.com by CDL-UC Riverside on 11/04/14…”

Section: B Female Genital Organsmentioning

confidence: 99%

Phospholipase A₂: Its Usefulness in Laboratory Diagnostics

Kaiser

1999

Critical Reviews in Clinical Laboratory Sciences

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Phospholipase A2 (PLA2) is an enzyme that catalyzes the hydrolysis of membrane phospholipids. This article reviews the source and structure of PLA2, the involvement of the enzyme in various biological and pathological phenomena, and the usefulness of PLA2 assays in laboratory diagnostics. Of particular importance is the role of PLA2 in the cellular production of mediators of inflammatory response to various stimuli. Assays for PLA2 activity and mass concentration are discussed, and the results of enzyme determinations in plasma from patients with different pathological conditions are presented. The determination of activity and mass concentration in plasma is particularly useful in the diagnosis and prognosis of pancreatitis, multiple organ failure, septic shock, and rheumatoid arthritis. A very important result is the demonstration that PLA2 is an acute phase protein, like CRP. Indeed, there is a close correlation between PLA2 mass concentration and CRP levels in several pathological conditions. Although the determination of C-reactive protein is much easier to perform and is routinely carried out in most clinical laboratories, the assessment of PLA2 activity or mass concentration has to be considered as a reliable approach to obtain a deeper understanding of some pathological conditions and may offer additional information concerning the prognosis of several disorders.

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Section: B Female Genital Organsmentioning

confidence: 99%

Phospholipase A₂: Its Usefulness in Laboratory Diagnostics

Kaiser

1999

Critical Reviews in Clinical Laboratory Sciences

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Arachidonic acid and cell signalling in the ovary and placenta

Boone¹,

Currie²,

Leung³

1993

Prostaglandins, Leukotrienes and Essential Fatty Acids

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Enhanced keratinocyte prostaglandin synthesis after UV injury is due to increased phospholipase activity

Kang-Rotondo

Miller

Morrison

et al. 1993

American Journal of Physiology-Cell Physiology

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The possibility that increased eicosanoid synthesis in skin after ultraviolet light irradiation is due to enhanced phospholipase activity was examined. [3H]arachidonic acid-labeled human keratinocyte cultures exposed to 30 mJ/cm2 ultraviolet (UV) B were studied 6 h after injury. Bradykinin-stimulated release of [3H]arachidonic acid was increased 1.8-fold over release from control cultures by prior irradiation. In unlabeled cultures, prior irradiation produced a threefold increase in bradykinin-stimulated prostaglandin (PG) E2 synthesis as measured by immunoassay. The relative contribution of increased phospholipase vs. cyclooxygenase activity was therefore examined using stable isotope mass measurements of PGE2. By this method, prior irradiation increased bradykinin-stimulated phospholipase activity 3.5-fold, while no change in total cellular cyclooxygenase activity was observed. The effects of irradiation on phospholipase activity were then assessed in more detail. The activities of phospholipase A2, arachidonoyl-CoA synthetase, and arachidonoyl-CoA lysophosphatide acyltransferase in cell homogenates were determined. No effect of UV exposure on the activity of these enzymes was observed. These results suggest that the increase in prostaglandin synthesis produced after UV irradiation is due to increased phospholipase activity, thus enhancing arachidonate release.

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Lipocortins and phospholipase A2 regulation

Cited by 3 publications

References 2 publications

Phospholipase A₂: Its Usefulness in Laboratory Diagnostics

Phospholipase A₂: Its Usefulness in Laboratory Diagnostics

Arachidonic acid and cell signalling in the ovary and placenta

Enhanced keratinocyte prostaglandin synthesis after UV injury is due to increased phospholipase activity

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Lipocortins and phospholipase A2 regulation

Cited by 3 publications

References 2 publications

Phospholipase A2: Its Usefulness in Laboratory Diagnostics

Phospholipase A2: Its Usefulness in Laboratory Diagnostics

Arachidonic acid and cell signalling in the ovary and placenta

Enhanced keratinocyte prostaglandin synthesis after UV injury is due to increased phospholipase activity

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Product

Resources

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Phospholipase A₂: Its Usefulness in Laboratory Diagnostics

Phospholipase A₂: Its Usefulness in Laboratory Diagnostics