Lipolytic activity of Antarctic cold-adapted marine bacteria (Terra Nova Bay, Ross Sea)

Abstract

Aims: The aim of this study was to investigate the lipolytic activity of cold‐adapted Antarctic marine bacteria and, furthermore, the combined effect of some environmental factors on this enzymatic process. Methods and Results: Strains were assayed for lipolytic activity on a basal medium amended with seven individual fatty acid esters. A significant activity was observed for 148 isolates (95·5% of the total screened). The interactive effect of pH, temperature and NaCl concentration on the substrates was tested for six representative isolates, identified as Pseudoalteromonas, Psychrobacter and Vibrio. Differences between strains according to NaCl and pH tolerances were observed. Only one strain degraded the substrate more efficiently at 4°C than at 15°C. Conclusions: Our findings demonstrate that the lipolytic activity of Antarctic marine bacteria is rather variable, depending on culture conditions, and occurs in a wide range of salt concentration and pH. Significance and Impact of the Study: Isolation and characterization of bacteria that are able to efficiently remove lipids at low temperatures will provide insight into the possibility to use cold‐adapted bacteria as a source of exploitable enzymes. Moreover, research on the interactive effects of salt concentration, pH and temperature will be useful to understand the true enzyme potentialities for industrial applications.

Characterization of Antarctic psychrotrophic bacteria with antibacterial activities against terrestrial microorganisms

“…Isolates were characterized by means of a combination of phenotypic tests as previously described in Bruni et al [18] and Lo Giudice et al [22]. All media used in this study were sterilized by autoclaving at 121 °C for 15 min and the plates were incubated at 15 °C for 14 days.…”

Gene cloning, expression and characterization of a cold-adapted lipase from a psychrophilic deep-sea bacterium Psychrobacter sp. C18

“…Cold adapted lipases have been isolated from marine psychrophilic and psychrotolerant bacteria such as Aeromonas (Lee et al, 2003), Moraxella (Feller et al, 1990(Feller et al, , 1991, Moritella (Yang et al, 2008), Photobacterium (Ryu et al, 2006), Pseudoalteromonas (Zeng et al, 2004;Lo Giudice et al, 2006;de Pascale et al, 2008), Pseudomonas (Zhang & Zeng, 2008), and Psychrobacter (Arpigny et al, 1993(Arpigny et al, , 1995Yumoto et al, 2003;Zeng et al, 2004;Zhang et al, 2007). However, psychrophilic or psychrotolerant bacteria usually grow slowly and their lipase production is limited.…”

Isolation, Partial Purification and Characterization of a Cold Active Lipase from Pseudomonas sp., Isolated from Satopanth Glacier of Western Himalaya, India

“…After that, seven isolates were screened for extracellular lipase production on tributyrin agar plate. Antarctic marine, glaciers, arctic and polar region that producing lipase enzymes [25][26][27].…”