Lipolytic Effect of Sparassis crispa Extracts in Differentiated 3T3-L1 Cells and High Fat Diet-induced Obese Mice

Lee, Mi-Ae; Park, Jin-Kyung; Um, Mihyang; Jeon, Jung-Woo; Lee, Jungmin; Park, Yoo Kyoung

doi:10.3746/jkfn.2012.41.12.1708

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…These results showed that SC efficiently inhibited fat accumulation in liver and epididymal adipocyte tissues. Moreover, our results substantiated the previous study that SC water extract enhanced lipolysis and up-regulated the expression of lipolytic enzymes such as CPT-1 and UCP-2 in differenciated 3T3-L1 cell [15].…”

Section: Histopathologysupporting

confidence: 91%

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Anti-obesity Effects of Sparassis crispa on High-fat Diet-induced Obese Mice

Lee¹,

Hou²,

Begum³

et al. 2014

Journal of Life Science

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The present study investigated the anti-obesity effects of Sparassis crispa (SC) on mice fed a high-fat (HF, 45 kcal% fat) diet. Mice were fed either a normal control diet and an HF diet or an HF diet supplemented with SC (1%, 3%, and 5%) for 12 weeks. The consumption of an HF diet compared to the NC group resulted in increases in body weight, the food efficiency ratio (FER), retroperitoneal and subcutaneous fat weights, cholesterol and triglyceride levels, fecal fat, and liver lipids. However, the administration of SC significantly decreased body weight gain, food intake, FER, cholesterol and triglyceride levels, and liver lipids in a dose-dependent manner. In particular, treatment with 5% SC significantly reduced the occurrence of fatty liver deposits and steatosis, which are associated with the increased adipocyte size in mice fed an HF diet. Therefore, these results suggested that dietary supplementation with SC exerts anti-obesity effects and could be used as a functional food to control obesity.

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Section: Histopathologysupporting

confidence: 91%

“…The 1% SC group showed the highest HDL-C value, while the 5% SC group exhibited the lowest HDL-C serum value. This result is inconsistent with Lee et al [15]. However, Tirupathi Pichiah et.…”

Section: Adipose Tissue Weightcontrasting

confidence: 60%

Anti-obesity Effects of Sparassis crispa on High-fat Diet-induced Obese Mice

Lee¹,

Hou²,

Begum³

et al. 2014

Journal of Life Science

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show abstract

“…Previous studies showed that 3T3-L1 cells differentiate into adipocytes when induced with lipid differentiation media consisting of insulin, dexamethasone, and isobutyl methylxanthine. Differentiation into adipocytes leads to regulation of genes that are expressed specifically in adipose tissue cells; these factors are expressed in the differentiation process [ 31 ]. In the case of NAFLD, HepG2 cells were treated with FFAs, to observe the changes in their accumulation of triglyceride (TG) [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Study of the Effects of Light Controlled Germination Conditions on Saponarin Content in Barley Sprouts and Lipid Accumulation Suppression in HepG2 Hepatocyte and 3T3-L1 Adipocyte Cells Using Barley Sprout Extracts

Kim

Jeong

et al. 2020

Molecules

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Barley sprouts (BS) contain physiologically active substances and promote various positive physiological functions in the human body. The levels of the physiologically active substances in plants depend on their growth conditions. In this study, BS were germinated using differently colored LED lights and different nutrient supplements. Overall, there were 238 varied BS samples analyzed for their total polyphenol and flavonoid contents. Principal component analysis (PCA) was performed to determine the relationship between the germinated samples and their total polyphenol and flavonoid contents, and those with high levels were further analyzed for their saponarin content. Based on the PCA plot, the optimal conditions for metabolite production were blue light with 0.1% boric acid supplementation. In vitro experiments using the ethanol extract from the BS cultured in blue light showed that the extract significantly inhibited the total lipid accumulation in 3T3-L1 adipocytes and the lipid droplets in HepG2 hepatocytes. These findings suggest that specific and controlled light source and nutrient conditions for BS growth could increase the production of secondary metabolites associated with inhibited fat accumulation in adipocytes and hepatocytes.

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Widdrol-induced lipolysis is mediated by PKC and MEK/ERK in 3T3-L1 adipocytes

et al. 2015

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Obesity is a serious medical condition causing various diseases such as heart disease, type-2 diabetes, and cancer. Fat cells (adipocytes) play an important role in the generation of energy through hydrolysis of lipids they accumulate. Therefore, induction of lipolysis (breakdown of lipids into fatty acids and glycerol), is one of the ways to treat obesity. In the present study, we investigated the lipolytic effect of widdrol in 3T3-L1 adipocytes and its mechanism. Widdrol considerably increased the amount of glycerol released from 3T3-L1 adipocytes into the medium in a time- and dose-dependent manner. To determine the mechanism of this effect, we investigated the alterations in glycerol release and protein expression in 3T3-L1 adipocytes treated with widdrol alone or widdrol and inhibitors of proteins involved in the cAMP-dependent pathway or cAMP-independent PKC-MAPK pathway, which are known to induce lipolysis in adipocytes. The adenylyl cyclase inhibitor SQ-22536, PLA2 inhibitor dexamethasone, PI3K inhibitor wortmannin, and PKA inhibitor H-89, which were used to investigate the involvement of the cAMP-dependent pathway, did not affect the lipolytic effect of widdrol. Widdrol-induced phosphorylation of PKC, MEK, and ERK, which are related to the PKC-MAPK pathway, and their phosphorylation was inhibited by their inhibitors (H-7, U0126, and PD-98059, respectively). Moreover, the increase in glycerol release induced by widdrol was almost completely blocked by PKC, MEK, and ERK inhibitors. These results suggest that widdrol induces lipolysis through activation of the PKC-MEK-ERK pathway.

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Lipolytic Effect of Sparassis crispa Extracts in Differentiated 3T3-L1 Cells and High Fat Diet-induced Obese Mice

Cited by 5 publications

References 15 publications

Anti-obesity Effects of Sparassis crispa on High-fat Diet-induced Obese Mice

Anti-obesity Effects of Sparassis crispa on High-fat Diet-induced Obese Mice

Comparative Study of the Effects of Light Controlled Germination Conditions on Saponarin Content in Barley Sprouts and Lipid Accumulation Suppression in HepG2 Hepatocyte and 3T3-L1 Adipocyte Cells Using Barley Sprout Extracts

Widdrol-induced lipolysis is mediated by PKC and MEK/ERK in 3T3-L1 adipocytes

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