Lipomatous muscular ‘dystrophy’ of Piedmontese cattle

Biasibetti, Elena; Amedeo, S.; Brugiapaglia, Alberto; Destefanis, G.; Stasio, Liliana Di; Valenza, F.; Capucchio, M.T.

doi:10.1017/s175173111200081x

Cited by 11 publications

(11 citation statements)

References 27 publications

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“…“Muscle inflammation” is generally observed at the musculus trapezius and longissimus dorsi of fattening Wagyu cattle; these musculatures contain the largest amount of intramuscular adipose tissue among bovine muscles [ 18 , 19 ]. The histological analysis of bovine “muscle inflammation” is characterized by muscular steatosis [ 4 , 33 ]. A murine model of nonalcoholic fatty liver disease studies indicated that the expression of p53 was increased in hepatic steatosis [ 13 , 53 ].…”

Section: Discussionmentioning

confidence: 99%

Fat depot-specific differences of macrophage infiltration and cellular senescence in obese bovine adipose tissues

Yamada

Kamiya

Higuchi

et al. 2018

The Journal of Veterinary Medical Science

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Obesity is associated with the chronic inflammation and senescence of adipose tissues. Macrophage is a key mediator of chronic inflammation that infiltrates obese adipose tissue and stimulates metabolic disorders. However, the fat depot-specific differences of macrophage infiltration and senescence, especially the influence on intramuscular adipose tissue, have remained unclear. We investigated the fat depot-specific differences of macrophage infiltration and senescence in obese bovine adipose tissue from three different anatomical sites (subcutaneous, intramuscular and visceral). Macrophage infiltrations and crown-like structures were observed in visceral adipose tissue, although there were few macrophages in subcutaneous and intramuscular adipose tissues. The positive reaction of senescence marker SA-βgal activity was observed in visceral adipose tissue. In contrast, the activity of SA-βgal in subcutaneous and intramuscular adipose tissues were low. The expression of p53 gene, the master regulator of cellular senescence, in visceral adipose tissue was higher than that of subcutaneous and intramuscular adipose tissue. At the cellular level, p53 gene expression was negatively correlated with the size of subcutaneous adipocytes. In contrast, p53 gene expressions were positively correlated with the size of intramuscular and visceral adipocytes. These results indicate that anatomical sites of obese adipose tissue affect macrophage infiltration and the senescent state in a fat depot-specific manner.

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Section: Discussionmentioning

confidence: 99%

Fat depot-specific differences of macrophage infiltration and cellular senescence in obese bovine adipose tissues

Yamada

Kamiya

Higuchi

et al. 2018

The Journal of Veterinary Medical Science

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“… This prototype can be used for diagnosis of cattle diseases, in addition to marbling measurements, because it is well-known that T 1 and T 2 values are different between normal and abnormal tissues [ 27 ]. For example, the diagnosis of muscular diseases [ 28 , 30 , 32 ] for live cattle would be made possible by in vivo relaxometry using the NMR surface scanner. …”

Section: Discussionmentioning

confidence: 99%

Development of a Single-Sided Nuclear Magnetic Resonance Scanner for the In Vivo Quantification of Live Cattle Marbling

Nakashima

2015

Appl Magn Reson

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Non-invasive in vivo marbling quantification helps owners to choose the optimum nutritional management for growing cattle and buyers to more precisely evaluate grown cattle at auctions. When using time-domain proton nuclear magnetic resonance (NMR) relaxometry, it is possible to quantify muscle and fat separately by taking advantage of the difference in the spin–spin relaxation time (T2) between water molecules in muscles and fat molecules, which would contribute to the non-invasive and objective determination of marbling scores. With this in mind, we developed a prototype NMR scanner (4.1 MHz for protons) using an original single-sided magnetic circuit and a plane radio frequency (RF) coil for use in the non-invasive quantification of water and fat in live cattle. The sensed region of the developed scanner is compact and almost cubical (19 × 19 × 16 mm3) while the investigation depth (the distance from the RF coil to the center of the sensed region) has been lengthened to 30 mm, which is sufficient for the in vivo trapezius muscle measurement of live cattle. Measurements of 17 samples of beef meat blocks kept at 39 °C were taken in a laboratory to successfully obtain the calibration lines used to convert the NMR signals into water and fat weight fractions at correlation coefficients in excess of 0.9. We also showed that each meat sample could be measured in about 10 s with a measurement error as small as approximately 10 wt%. Accordingly, we believe that our prototype scanner would be useful for in vivo marbling measurements of live cattle trapezius muscles.Electronic supplementary materialThe online version of this article (doi:10.1007/s00723-015-0657-4) contains supplementary material, which is available to authorized users.

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“…Lesions observed were measured semiquantitatively using a scoring system based on the characterization of the inflammatory cell infiltrate, specifically by accumulation of PMNs, according to the literature [ 21 ]. A scale from 1 to 4 was applied to the following alterations: 0, no lesions, absence of interstitial and/or alveolar inflammatory cell infiltrate and undamaged tissue; 1, focal to multifocal, mild interstitial and/or alveolar infiltrate of inflammatory cells and undamaged tissue; 2, multifocal, moderate interstitial and/or alveolar infiltrate of inflammatory cells and undamaged tissue; 3, severe, diffuse interstitial and/or alveolar infiltrate of inflammatory cells and focal areas of tissue damage; 4, severe, diffuse interstitial and/or alveolar infiltrate of inflammatory cells and extensive necrotic areas.…”

Section: Methodsmentioning

confidence: 99%

A mouse mastitis model to study the effects of the intramammary infusion of a food-grade Lactococcus lactis strain

et al. 2017

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Lactococcus lactis is one of the most important microorganisms in the dairy industry and has “generally recognized as safe” (GRAS) status. L. lactis belongs to the group of lactic acid bacteria (LAB) and is encountered in a wide range of environments. Recently, the use of the intramammary infusion of a live culture of LAB has been investigated as a new antibiotic alternative for treating mastitis in dairy ruminants. Controversial results are described in literature regarding its efficacy and safety. In this study we conducted in-depth investigation of the mammary gland immune response induced by intramammary inoculum of a live culture of L. lactis LMG 7930 using the mouse mastitis model. Overnight cultures either of L. lactis (≈ 107 CFU) or of the mastitis pathogens Staphylococcus chromogenes (≈ 105 CFU) or S. aureus (≈ 102 CFU/ml) were injected into the mouse inguinal glands. A double injection, consisting of S. chromogenes first and then L. lactis, was also investigated. Bacterial recovery from the gland and inflammatory cell infiltration were assessed. L. lactis-treated and control glands were analysed for proinflammatory cytokine production. Microbiological results showed that L. lactis was able to survive in the mammary gland 24 h post infection, as were the mastitis pathogens S. chromogenes and S. aureus. L. lactis reduced S. chromogenes survival in the glands and increased its own survival ability by coexisting with the pathogen. Histology showed that L. lactis-treated glands presented variable histological features, ranging from undamaged tissue with no inflammatory cell infiltrate to severe PMN infiltrate with focal areas of tissue damage. S. aureus-treated glands showed the most severe histological grade of inflammation despite the fact that the inoculum size was the smallest. In contrast, most S. chromogenes-treated glands showed normal structures with no infiltration or lesions. Significant increases in IL-1β and TNF-α levels were also found in L. lactis-inoculated glands. The above findings seem to suggest that food-grade L. lactis at a high-inoculum dose such as an overnight culture may elicit a suppurative inflammatory response in the mammary gland, thus becoming a potential mastitis-causing pathogen. Because of the unpredictable potential of L. lactis in acting as a potential mastitis pathogen, this organism cannot be considered a safe treatment for bovine mastitis.

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Lipomatous muscular ‘dystrophy’ of Piedmontese cattle

Cited by 11 publications

References 27 publications

Fat depot-specific differences of macrophage infiltration and cellular senescence in obese bovine adipose tissues

Fat depot-specific differences of macrophage infiltration and cellular senescence in obese bovine adipose tissues

Development of a Single-Sided Nuclear Magnetic Resonance Scanner for the In Vivo Quantification of Live Cattle Marbling

A mouse mastitis model to study the effects of the intramammary infusion of a food-grade Lactococcus lactis strain

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