Lipoperoxides in plasma as measured by liquid-chromatographic separation of malondialdehyde-thiobarbituric acid adduct.

Abstract

This assay of plasma lipoperoxides involves hydrolysis in dilute H3PO4 at 100 degrees C; complexation of malondialdehyde (MDA), a hydrolysis product, with thiobarbituric acid (TBA); methanol precipitation of plasma proteins; fractionation of the protein-free extract on a C18 column; and spectrophotometric quantification of the MDA-TBA adduct at 532 nm. The detection limit was 0.15 mumol of MDA per liter of plasma. Run-to-run precision (CV) averaged 8 to 13%. Analytical recovery of MDA after addition of tetraethoxypropane standards to 21 specimens of human or rat plasma averaged 98% (SD 7%). Lipoperoxide concentrations (as MDA) averaged 0.60 (SD 0.13) mumol/L in plasma specimens from 41 healthy persons and 1.4 (SD 0.3) mumol/L in plasma specimens from 12 control rats. Mean lipoperoxide concentrations were 1.5 to 2.3 times as great in plasma sampled from rats one to three days after subcutaneous administration of NiCl2 at dosages (250 to 750 mumol per kilogram body wt) previously shown to induce lipid peroxidation in lung, liver, and kidney.

Plasma and platelet ascorbate pools and lipid peroxidation in insulin‐dependent diabetes mellitus

“…1). The reference values for plasma MDA in control adults were consistent with those of Wong et al [22].…”

“…Protein-free supernatants were stored at ¹80ЊC and AA and DHA were measured in duplicate using HPLC according to Dhariwal et al [21]. MDA was also analysed using HPLC [22]. Both intra-and interassay CVs were 3·4% and 4% for AA, 5·2% and 5·9% for DHA, and 4·9% and 5·7% for MDA.…”

Anti‐fibrosclerotic effects of shock wave therapy in lipedema and cellulite

“…MDA was determined according to Wong et al [19] with modifications of Sommerburg et al [20]. MDA was determined as the thiobarbituric acid (TBA) derivative.…”

Protective action of hydroxyethyl rutosides on singlet oxygen-challenged cardiomyocytes

“…Accumulation of secondary lipid peroxidation products reacting with thiobarbituric acid was measured spectrophotometrically according to a method described by Wong et al (1987). Plasma lipoperoxides were hydrolysed by boiling in dilute phosphoric acid (0.44 M H3PO4).…”