2023
DOI: 10.1021/acs.bioconjchem.3c00204
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Lipophilic Anchors that Embed Bioconjugates in Bilayer Membranes: A Review

Abstract: A wide range of biomaterials and engineered cell surfaces are composed of bioconjugates embedded in liposome membranes, surface-immobilized bilayers, or the plasma membranes of living cells. This review article summarizes the various ways that Nature anchors integral and peripheral proteins in a cell membrane and describes the strategies devised by chemical biologists to label a membrane protein in living cells. Also discussed are modern synthetic and semisynthetic methods to produce lipidated proteins. Subseq… Show more

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Cited by 5 publications
(4 citation statements)
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“… 24 Further, the lack of extensive lipophilic chains can also prevent AztecM-LD from being incorporated into lipid bilayers, thereby increasing its specificity for lipid monolayers such as LDs. 25 Then, the photoinduced electron transfer (PET) process is modulated from the NIR fluorescence to the green coumarin 343 type emission activated after intramolecular spirocyclization, Figure 1 A. Interestingly, the dual localization distribution of the open and spirocyclic forms colocalized for mitochondria and lipid droplets, respectively, Figure 2 . The corresponding colocalization analysis using MitoLite Blue and NileBlue commercial dyes ( Figure S15 ) confirmed the specific targeting ability of AztecM-LD and its hydrolyzed form AztecH –1 , respectively.…”
Section: Resultsmentioning
confidence: 98%
See 1 more Smart Citation
“… 24 Further, the lack of extensive lipophilic chains can also prevent AztecM-LD from being incorporated into lipid bilayers, thereby increasing its specificity for lipid monolayers such as LDs. 25 Then, the photoinduced electron transfer (PET) process is modulated from the NIR fluorescence to the green coumarin 343 type emission activated after intramolecular spirocyclization, Figure 1 A. Interestingly, the dual localization distribution of the open and spirocyclic forms colocalized for mitochondria and lipid droplets, respectively, Figure 2 . The corresponding colocalization analysis using MitoLite Blue and NileBlue commercial dyes ( Figure S15 ) confirmed the specific targeting ability of AztecM-LD and its hydrolyzed form AztecH –1 , respectively.…”
Section: Resultsmentioning
confidence: 98%
“…The probe structure is based on a coumarin-merocyanine hybrid containing an ester fragment as a hydrolyzable function upon esterase activity similar to alkanoyloxymethyl esters . Further, the lack of extensive lipophilic chains can also prevent AztecM-LD from being incorporated into lipid bilayers, thereby increasing its specificity for lipid monolayers such as LDs . Then, the photoinduced electron transfer (PET) process is modulated from the NIR fluorescence to the green coumarin 343 type emission activated after intramolecular spirocyclization, Figure A.…”
Section: Resultsmentioning
confidence: 99%
“…Specifically, the NK cell lytic-immunological synapse is activated by a series of sequential events: (1) interaction of NK-to-target cell interfaces, (2) secretion of cytotoxic granules and cytokines, and (3) target cell lysis . In the structure of DSPE PEG -diPEG-LBA, DSPE lipid tail is anchored into the NK cell membrane through hydrophobic interactions , for sufficient surface presentation of LBA molecules on NK cells. Subsequently, the head LBA moiety is extracellularly exposed for ASGPR targeting.…”
Section: Resultsmentioning
confidence: 99%
“…Consequently, the multifunctional SCCs constructed in this manner are typically hydrophobic and rigid structures. The hydrophobic nature of SCCs facilitates their rapid and firm binding to the lipophilic cell membrane surfaces, 61 enhancing their cellular uptake efficiency. However, this hydrophobicity leads to their rapid aggregation and precipitation in the aqueous environment of living organisms, posing challenges in maintaining their in vivo stability and biocompatibility.…”
Section: Strategies To Improve the Water Solubility And Biostability ...mentioning
confidence: 99%