Lipophorin of female Blattella germanica (L.): characterization and relation to hemolymph titers of juvenile hormone and hydrocarbons

Sevala, Veeresh L.; Shu, Shengqiang; Ramaswamy, S.; Schal, Coby

doi:10.1016/s0022-1910(98)00142-5

Cited by 45 publications

(37 citation statements)

References 71 publications

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“…Oenocytes are large abdominal cells that are rich in smooth endoplasmic reticulum and mitochondria and have been implicated in hydrocarbon and lipid synthesis (Chapman 1998;Fan et al 2002). Cuticular hydrocarbons and pheromones are produced by abdominal oenocytes and are shuttled through the hemolymph by high-density lipophorin (Gu et al 1995;Schal et al 1998;Sevala et al 1999). In mosquitoes, oenocytes might produce AT or might be a target for the peptide.…”

Section: Discussionmentioning

confidence: 99%

Immunostaining for allatotropin and allatostatin-A and -C in the mosquitoes Aedes aegypti and Anopheles albimanus

Hernández‐Martínez

Lanz‐Mendoza

et al. 2005

Cell Tissue Res

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Confocal laser-scanning microscopy was used to carry out a comparative study of the immunostaining for three families of neuropeptides, viz., allatostatin-A (AS-A), allatostatin-C (AS-C) and allatotropin (AT), in adult female mosquitoes of Aedes aegypti and Anopheles albimanus. The specific patterns of immunostaining for each of the three peptides were similar in both species. The antisera raised against AT, AS-A, and AS-C revealed intense immunoreactivity in the cells of each protocerebral lobe of the brain and stained cells in each of the ventral ganglia and neuronal projections innervating various thoracic and abdominal tissues. Only the AS-A antiserum labeled immunoreactive endocrine cells in the midgut. The distribution of the peptides supports the concept that they play multiple regulatory roles in both species.

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Section: Discussionmentioning

confidence: 99%

Immunostaining for allatotropin and allatostatin-A and -C in the mosquitoes Aedes aegypti and Anopheles albimanus

Hernández‐Martínez

Lanz‐Mendoza

et al. 2005

Cell Tissue Res

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“…Lipophorin polyclonal antibodies were raised in New Zealand White rabbits and radial imunodiffusion assays confirmed specificity to lipophorin and no cross-reactivity with other hemolymph proteins (Sevala et al, 1999). Hemolymph was collected from 6-day-old female nymphs, the hemocytes were removed (6000g for 10 min), and lipophorin was immunoprecipitated by addition of excess anti-lipophorin rabbit serum followed by Pansorbin cells.…”

Section: Lipophorin Purification and Extractionmentioning

confidence: 95%

“…The HC concentration in the hemolymph remains in a narrow range (7.6-9.0 g/l) throughout the stadium and through ecdysis (Fig. 4(a)), as it does in adult females through the reproductive cycle (Sevala et al, 1999). An early increase in nymphal hemolymph HC content (Fig.…”

Section: Hydrocarbon Transport Through the Hemolymphmentioning

confidence: 95%

“…Absorbance was read at 405 nm in a BioTek automated microplate reader. Specificity and validity of the assay have been demonstrated with serially diluted hemolymph, purified Blattella vitellin, and bovine serum albumin (Sevala et al, 1999). Protein titer was determined by the Bradford (1976) method (Bio-Rad, Richmond, CA), using BSA as a standard.…”

Section: Quantification Of Hemolymph Protein and Lipophorinmentioning

confidence: 99%

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Site of synthesis, tissue distribution, and lipophorin transport of hydrocarbons in Blattella germanica (L.) nymphs

Young

Bachmann

Sevala

et al. 1999

Journal of Insect Physiology

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“…Thus, during gestation, JH production remains low, but at the end of gestation, before egg hatch, JH titers slightly increase and the basal oocytes grow slightly as well (Gadot et al, 1989a;Gadot et al, 1989b). The average length of the basal oocytes is a reliable measure of the reproductive stage because all basal oocytes mature synchronously and their length correlates well with both the activity of the CA (as measured by their JH biosynthesis in vitro) and with JH titer in the hemolymph (as measured by GC-MS) (Sevala et al, 1999;Treiblmayr et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Differential physiological responses of the German cockroach to social interactions during the ovarian cycle

Uzsák

Schal

2012

Journal of Experimental Biology

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SUMMARYIn many animal species, social interactions can influence the morphology, physiology and behavior of individuals, including their rate of development and reproduction. Reproduction in cockroaches is regulated by juvenile hormone III (JH), and social interactions have been shown to accelerate female reproduction in the German cockroach, Blattella germanica (L.), by stimulating JH production. However, it is not clear in this or any other insect species whether social facilitation of the reproductive rate occurs throughout the ovarian cycle or only at certain stages. We compared the effects of social interactions during the preoviposition period (when JH production is high) and during gestation (when little JH is produced), as well as during the first ovarian cycle (when females are virgin) and the second ovarian cycle (after females had mated). Social interaction with one conspecific female was sufficient to accelerate JH production and oocyte maturation, but this effect was reversed by crowding. Social interactions also accelerated the onset of sexual receptivity in virgin females. However, social interactions failed to shorten gestation, suggesting that social cues stimulate JH production only when the corpora allata (CA) are active and not when CA activity is suppressed by the central nervous system. Females were most responsive to transient social isolation and transient social interactions when 2-3days old, suggesting that they are particularly sensitive to social interactions when their CA become active. Overall, these results show that all JH-dependent events in the reproductive cycle of B. germanica females are under the strong influence of social interactions.

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Lipophorin of female Blattella germanica (L.): characterization and relation to hemolymph titers of juvenile hormone and hydrocarbons

Cited by 45 publications

References 71 publications

Immunostaining for allatotropin and allatostatin-A and -C in the mosquitoes Aedes aegypti and Anopheles albimanus

Immunostaining for allatotropin and allatostatin-A and -C in the mosquitoes Aedes aegypti and Anopheles albimanus

Site of synthesis, tissue distribution, and lipophorin transport of hydrocarbons in Blattella germanica (L.) nymphs

Differential physiological responses of the German cockroach to social interactions during the ovarian cycle

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