Lipopolysaccharide- and β-1,3-glucan-binding protein from Litopenaeus vannamei: Purification, cloning and contribution in shrimp defense immunity via phenoloxidase activation

Phupet, Benjaporn; Pitakpornpreecha, Thanawat; Baowubon, Nuntaporn; Runsaeng, Phanthipha; Utarabhand, Prapaporn

doi:10.1016/j.dci.2017.11.016

Cited by 48 publications

(16 citation statements)

References 48 publications

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“…This complex then binds to receptors of the hemocytes and triggers the melanization and encapsulation reaction, the most powerful immune response of decapods that eliminates the pathogens. In shrimp Litopenaeus vannamei , the LGBP was shown to be synthesized in the hepatopancreas (Phupet, Pitakpornpreecha, Baowubon, Runsaeng, & Utarabhand, ). Its expression was upregulated after injection of Vibrio parahaemolyticus , white spot syndrome virus, lipopolysaccharide, and ß‐1,3‐glucan.…”

Section: Synthesis Of Hemolymph Proteins In Epithelial Cell Typesmentioning

confidence: 99%

Functional cytology of the hepatopancreas of decapod crustaceans

Vogt

2019

Journal of Morphology

176

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This article reviews the morphogenesis, morphology, histology, ultrastructure, and structural-functional relationships of the hepatopancreas, the main metabolic organ of the Decapoda. The hepatopancreas develops in early larval stages from a pair of lateral lobes of the midgut anlage. In adults, it consists of hundreds of blindly ending tubules that are enveloped by a muscle net consisting of longitudinal and circular fibers. Stem cells at the distal ends of the tubules give rise to three ultrastructurally different epithelial cell types, the R-, F-, and B-cells. Histochemistry, immunohistochemistry, in situ hybridization, and monitoring of ultrastructural changes under different experimental conditions allowed the attribution of functions to these cell types. R-cells serve for the absorption and metabolization of nutrients, storage of energy reserves and minerals, synthesis of lipoproteins for export to other organs, detoxification of heavy metals, and excretion of uric acid. F-cells synthesize digestive enzymes and blood proteins involved in oxygen transport and immune defense. They also detoxify some heavy metals and probably organic xenobiotics. B-cells are assumed to produce and recycle fat emulsifiers. The hepatopancreas tubules lack nerves. The presence of scattered M-cells with putative endocrine function in the epithelium suggests that the hepatopancreas is mainly hormonally controlled. M-cells probably represent a self-perpetuating cell lineage independent from E-cells. The interstitium between the tubules contains connective tissue, arterioles, hemolymph with circulating hemocytes, and fixed phagocytes that eliminate pathogens.The hepatopancreas is histologically and ultrastructurally uniform throughout the Decapoda, despite their broad variety in body size, morphology, life style, and ecology.However, in a few cavernicolous and deep-sea shrimps parts of the hepatopancreas are transformed into large oil storing and bioluminescent compartments. Within the malacostracan crustaceans, the hepatopancreas of the Decapoda is most similar to the digestive gland of the Euphausiacea, supporting close taxonomic relationship of these two taxa.

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Section: Synthesis Of Hemolymph Proteins In Epithelial Cell Typesmentioning

confidence: 99%

Functional cytology of the hepatopancreas of decapod crustaceans

Vogt

2019

Journal of Morphology

176

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“…Cells lysis and necrosis appeared at 3 h after shrimp injected with saline solution. However, the same situation was first found at 12 h after shrimp injected with LPS, the expression of LPS-related genes and regulated enzyme activities was increased to keep hepatopancreas normal (Mei et al, 2016;PF et al, 2009;Phupet et al, 2017;Soltani et al, 2019). Interestingly, the activities of AST, ALT and ALP began to increase after injection of LPS for 12 h in our study, which consistent with the results of histological analysis.…”

Section: Discussionmentioning

confidence: 71%

“…LPS may be effective in converting inactive proPO to active PO in haemocytes, and the transcription level of C‐MnSOD and CAT both in hepatopancreas and haemocytes was enhanced after LPS injection, indicating that LPS played an important role in shrimp against bacterial (PF et al, 2009). In summary, LPS triggered a series of immune responses leading to activation of the immune defence in the hepatopancreas and haemocytes of L. vannamei (PF et al, 2009; Phupet et al, 2017). However, the response of LvALF to LPS injection has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Lipopolysaccharide induced unfolded protein response and related anti‐lipopolysaccharide factor expression in Pacific white shrimp, Litopenaeus vannamei

Wang

Ning

et al. 2021

Aquaculture Research

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Litopenaeus vannamei (L. vannamei), also known as Pacific white shrimp, is one of the largest cultured shrimp species in the world, accounting for more than 70 per cent of global shrimp production for its rapid growth, tender meat and high nutritional value (Loker et al., 2010). Unfortunately, L. vannamei is susceptible to bacteria which can lead to massive death of shrimp, and cause huge economic losses to the farming industry (Walker & Winton, 2010). Therefore, it is necessary to properly understand the pathogenesis of bacterial

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“…As previously reported, the bGBP was found induced in the hepatopancreas of M. japonicus [62], P. styrilostris [71], and F. chinensis [72] after contact with WSSV. The treatment with dsRNA LvbGBP showed a 100% death rate 72 h after the WSSV injection when compared to 50% mortality in control group after the same time [73]. We also detected genes from the serine protease family, such as trypsin and chymotrypsin, to be up-regulated in the hepatopancreas of healthy shrimps.…”

Section: Discussionmentioning

confidence: 79%

Shedding the Light on Litopenaeus vannamei Differential Muscle and Hepatopancreas Immune Responses in White Spot Syndrome Virus (WSSV) Exposure

Santos

Andrade

Fernandes

et al. 2020

Genes

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White Spot Syndrome Virus (WSSV) is one of the main threats to farming Litopenaeus vannamei, the most important crustacean commercialized in aquaculture worldwide. Here, we performed RNA-seq analyses in hepatopancreas and muscle from WSSV-negative (healthy) and WSSV-positive (unhealthy) L. vannamei, previously exposed to the virus, to obtain new insights about the molecular basis of resistance to WSSV. We detected 71% of our reads mapped against the recently described L. vannamei genome. This is the first report mapping RNA-seq transcripts from shrimps exposed to WSSV against the species reference genome. Differentially expressed gene (DEG) analyses were performed for four independent comparisons, and 13,338 DEGs were identified. When the redundancies and isoforms were disregarded, we observed 8351 and 6514 DEGs, respectively. Interestingly, after crossing the data, we detected a common set of DEGs for hepatopancreas and healthy shrimps, as well as another one for muscle and unhealthy shrimps. Our findings indicate that genes related to apoptosis, melanization, and the Imd pathway are likely to be involved in response to WSSV, offering knowledge about WSSV defense in shrimps exposed to the virus but not infected. These data present potential to be applied in further genetic studies in penaeids and other farmed shrimp species.

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Lipopolysaccharide- and β-1,3-glucan-binding protein from Litopenaeus vannamei: Purification, cloning and contribution in shrimp defense immunity via phenoloxidase activation

Cited by 48 publications

References 48 publications

Functional cytology of the hepatopancreas of decapod crustaceans

Functional cytology of the hepatopancreas of decapod crustaceans

Lipopolysaccharide induced unfolded protein response and related anti‐lipopolysaccharide factor expression in Pacific white shrimp, Litopenaeus vannamei

Shedding the Light on Litopenaeus vannamei Differential Muscle and Hepatopancreas Immune Responses in White Spot Syndrome Virus (WSSV) Exposure

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