Lipopolysaccharide Downregulates 11β-Hydroxysteroid Dehydrogenase 2 Expression through Inhibiting Peroxisome Proliferator–Activated Receptor-γ in Placental Trophoblasts

Fu, Lin; Chen, Yuan-Hua; Bo, Qingli; Song, Yaping; Ma, Li; Wang, Bo; Xu, Shen; Zhang, Cheng; Wang, Hua; D, Xu

doi:10.4049/jimmunol.1900132

Cited by 28 publications

(19 citation statements)

References 61 publications

(72 reference statements)

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“…The article found that nuclear translocation of PPAR‐γ and NF‐κB p65 was remarkably elevated in mononuclear sinusoidal trophoblast giant cells of the labyrinth layer. Our recent study found that lipopolysaccharide exposure inhibited placental PPAR‐γ and its downstream gene 11β‐HSD2 via promoting the interaction between NF‐κB p65 and PPAR‐γ in the cytoplasm and nucleus of the placental trophoblast 14 . These results provide mechanistic explanation by which inflammation inhibits placental 11β‐HSD2 in SGA infants.…”

Section: Discussionmentioning

confidence: 76%

“…Peroxisome proliferator‐activated receptor gamma, a ligand‐activated nuclear receptor, involves in placental development. The in vitro studies demonstrated that activated PPAR‐γ upregulated 11β‐HSD2 in human placental trophoblasts 13,14 . A population‐based case‐control study found that PPAR‐γ protein and mRNA levels were decreased in SGA placentas 12 .…”

Section: Discussionmentioning

confidence: 99%

“…According to a case‐control study, placental 11β‐HSD2 expression is positively correlated with PPAR‐γ level in pregnancies complicated by preeclampsia 13 . The in vitro study showed that activated PPAR‐γ upregulated the expression of 11β‐HSD2 in human placental trophoblasts 13,14 . Nuclear factor‐kappa B (NF‐κB) is a transcription factor that regulates inflammatory signaling and inflammatory cytokines, such as tumour necrosis factor (TNF)‐α and interleukin (IL)‐1β 15 .…”

Section: Introductionmentioning

confidence: 99%

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Association among placental 11β‐HSD2, PPAR‐γ, and NF‐κB p65 in small‐for‐gestational‐age infants: A nested case‐control study

Gan

et al. 2020

American J Rep Immunol

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Problem: 11β-Hydroxysteroid dehydrogenase 2 (11β-HSD2) catalyzes active glucocorticoids into their inactive products, preventing the passage of glucocorticoids into the fetus from maternal circulation. Peroxisome proliferator-activated receptor (PPAR)γ is a member of the nuclear receptor superfamily that regulates the expression of placental 11β-HSD2. Nuclear factor-kappa B (NF-κB) is a transcription factor that regulates inflammatory signaling. This study aimed to investigate the association among 11β-HSD2, PPAR-γ, and NF-κB p65 in small-for-gestational-age (SGA) infants. Method of study:Forty-six SGA and 46 appropriate-for-gestational-age (AGA) infants were enrolled in this study. Both newborns and placentas were weighed.Placental 11β-HSD2 levels were measured using Western blotting. Placental PPAR-γ and NF-κB p65 were detected by immunohistochemistry. Placental inflammatory cytokines were evaluated by real-time RT-PCR.Results: 11β-HSD2 levels were lower in SGA placentas than those in AGA placentas.Placental PPAR-γ-positive nuclei were less in SGA than those in AGA. By contrast, placental NF-κB p65-positive nuclei were more in SGA than those in AGA. The levels of CRP, , several inflammatory cytokines, were higher in SGA placentas. Correlation analysis showed that neonatal weight was positively associated with PPAR-γ and 11β-HSD2 in SGA placentas. By contrast, neonatal weight was inversely correlated with NF-κB p65 in SGA placentas. 11β-HSD2 was positively correlated with PPAR-γ in SGA placentas.

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Association among placental 11β‐HSD2, PPAR‐γ, and NF‐κB p65 in small‐for‐gestational‐age infants: A nested case‐control study

Gan

et al. 2020

American J Rep Immunol

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“…Placental 11β-hydroxysteroid dehydrogenase 2 (HSD2), a glucocorticoid-catalysing enzyme, protects against foetal growth restriction by preventing active glucocorticoids present in the maternal circulation from entering the foetal circulation. In a recent study, bacterial LPS was shown to downregulate 11β-HSD2 by suppressing proliferator-activated receptor-γ in placental trophoblasts 27 . This may contribute to excessive glucocorticoid production, resulting in LPS-induced foetal growth restriction.…”

Section: Discussionmentioning

confidence: 97%

Radiological screening of maternal periodontitis for predicting adverse pregnancy and neonatal outcomes

Heo

Ahn

Park

2020

Sci Rep

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It is well known that periodontitis, diagnosed mainly by periodontal probing, is associated with adverse pregnancy outcomes. However, periodontal probing is time-consuming, highly discomforting, inaccurate, and invasive. We aimed to assess whether periodontitis severity based on radiological staging in accordance with the 2017 new consensus classification was related to adverse pregnancy and neonatal outcomes. The medical records of 165 mothers who underwent panoramic radiography within 5 years before and after the time of delivery and of their singleton neonates were retrospectively reviewed. Twenty-two mothers (13.3%) had severe periodontitis (SP), and 143 (86.7%) had mild or moderate periodontitis (MP). In relation to adverse pregnancy outcomes, uterine leiomyoma (18.2% vs. 4.2%, P = 0.029), chronic hypertension (9.1% vs. 0.7%, P = 0.047), and preeclampsia (13.6% vs. 2.1%, P = 0.032) occurred significantly more frequently in the SP group than in the MP group. The incidences of very preterm birth (13.6% vs. 1.4%, P = 0.017), extremely preterm birth (9.1% vs. 0.7%, P = 0.047), and small for gestational age (22.7% vs. 5.6%, P = 0.017) were also significantly higher in the SP group than in the MP group. Radiological screening of maternal periodontitis could be useful for predicting adverse pregnancy and neonatal outcomes as well as diagnosing SP in pregnant women.

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“…Human S100A8 and scrambled siRNAs were obtained from Gene Pharma Corp (Shanghai, China). The S100A8 siRNA sequences was as follows: (sense, 5′-GGUCACUACUGAGUGCCCUCAGUUU--3′; antisense, 5′-AAACUGAGGGCACUCAGUAGUGACC-3′); The RNA interference protocol was on the basis of the previous study [24]. Human S100A8-siRNA was transfected into cells using Lipofectamine 3000 according to the manufacturer's instructions (Invitrogen, Carlsbad, CA, United States).…”

Section: Small Interfering Rna (Sirna) Transfectionmentioning

confidence: 99%

Serum S100A8 as an early diagnostic biomarker in patients with community-acquired pneumonia

Zheng

Fei

et al. 2020

Preprint

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Background and Objectives Limited studies suggested that calprotectin may take part in the pathophysiology of community-acquired pneumonia (CAP). Nevertheless, there is no clinical study to analyze the role of S100A8 in CAP patients. The objective of this study was to analyze the association of serum S100A8 with the severity of CAP based on a cross-sectional study. Methods Entire 200 CAP patients and 100 normal subjects were recruited. Demographic data, clinical information and serum were collected on admission. Serum S100A8 and inflammatory cytokines were detected. Results Serum S100A8 was increased in CAP patients on admission. Serum S100A8 was gradually increased in parallel with the CAP severity scores. Serum S100A8 was positively correlated with CAP severity scores (CURB-65, CRB-65, PSI, CURXO and SMART-COP), blood routine parameters (WBC, neutrophil-lymphocyte ratio and monocyte-lymphocyte ratio) and inflammatory cytokines (TNFα, IL-1β and CRP). Furtherly, univariate and multivariate logistical regression analysis revealed that there was a positive association between serum S100A8 with CRB-65, PSI and CURXO. Moreover, the predictive capacity of serum S100A8 was performed by receiver operating characteristic area under the curve (AUC) analysis. The AUCs of S100A8 for CAP and CAP severity were 0.855 and 0.893, respectively. Mechanistic analysis found that S100A8 knockdown alleviated streptococcus pneumoniae-evoked inflammatory cytokines in A549 cells. Conclusion Serum S100A8 on admission was positively associated with the severity of CAP. S100A8 knockdown alleviates streptococcus pneumoniae- evoked inflammatory cytokines in A549 cells, indicating that S100A8 may exert an important role in the pathophysiology of CAP and be an early serum diagnostic biomarker for CAP.

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Lipopolysaccharide Downregulates 11β-Hydroxysteroid Dehydrogenase 2 Expression through Inhibiting Peroxisome Proliferator–Activated Receptor-γ in Placental Trophoblasts

Cited by 28 publications

References 61 publications

Association among placental 11β‐HSD2, PPAR‐γ, and NF‐κB p65 in small‐for‐gestational‐age infants: A nested case‐control study

Association among placental 11β‐HSD2, PPAR‐γ, and NF‐κB p65 in small‐for‐gestational‐age infants: A nested case‐control study

Radiological screening of maternal periodontitis for predicting adverse pregnancy and neonatal outcomes

Serum S100A8 as an early diagnostic biomarker in patients with community-acquired pneumonia

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