Lipopolysaccharide-induced failure of the gut barrier is site-specific and inhibitable by growth hormone

Yue, Chao; Wang, Wei; Tian, Weiliang; Huang, Qian; Zhao, Risheng; Zhao, Yunzhao; Li, Qiurong; Li, Jieshou

doi:10.1007/s00011-013-0593-4

Cited by 25 publications

(21 citation statements)

References 38 publications

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“…Therefore, the FD4 clearance is also employed as a good marker for intestinal mucosal barrier function. 25,36) In the present study, we found that BBR caused decreases in both serum D-lactate level and intestinal clearance of FD4. In addition, although there was no significant changes in histopathology among groups, the Chiu's scores assessed for intestinal mucosal injury were significantly decreased by BBR when compared with the peritoneal air exposure group.…”

Section: Discussionsupporting

confidence: 59%

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Berberine Ameliorates Intestinal Mucosal Barrier Damage Induced by Peritoneal Air Exposure

Tan

Zhou

et al. 2015

Biol. Pharm. Bull.

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Berberine, an isoquinoline alkaloid derived from many medicinal plants, has been extensively used to treat various gastrointestinal diseases. In the present study, we investigated whether berberine could ameliorate intestinal mucosal barrier damage induced by peritoneal air exposure for 3 h. Peritoneal air-exposure rats received 100, 150, and 200 mg/kg berberine orally via gavage four times before and after surgery. Blood and terminal ileum samples were collected 24 h after surgery. The serum D-lactate levels were determined using an enzyme-linked immunosorbent assay (ELISA) kit. Intestinal permeability was determined by measuring the intestinal clearance of fluorescein isothiocyanate (FITC)-dextran (FD4). Intestinal inflammation was assessed by measuring myeloperoxidase activity. Intestinal histopathology was also assessed. The results revealed that berberine decreased the serum D-lactate level, intestinal FD4 clearance, and myeloperoxidase activity. Edema and inflammation were reduced by berberine in the intestinal mucosa and submucosa, and the Chiu's scores, indices of intestinal mucosal injury, also decreased in the berberine-treated group. In addition, berberine exerted these protective effects in a dose-dependent manner, with a significant difference from the control group at doses of 150 and 200 mg/kg. The results suggest that berberine could ameliorate intestinal mucosal barrier damage induced by peritoneal air exposure, which is linked to its anti-inflammatory activity. Berberine may be a promising treatment for intestinal mucosal barrier damage in open abdominal surgery.

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Section: Discussionsupporting

confidence: 59%

“…15,25) Briefly, a segment of terminal ileum with length of 8 cm was prepared, and the mucosa was everted. One end of the intestinal segment was ligated, and a gut sac was made by injecting 1.0 mL of KrebsHenseleit bicarbonate buffer from the other end.…”

Section: D-lactate (D-la) Determinationmentioning

confidence: 99%

Berberine Ameliorates Intestinal Mucosal Barrier Damage Induced by Peritoneal Air Exposure

Tan

Zhou

et al. 2015

Biol. Pharm. Bull.

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“…LPS, an endotoxin produced by bacteria, is known to induce ER stress (32)(33)(34). It can directly cause intestinal epithelial cell apoptosis (35) and failure of intestinal barrier (36). Thus, we used CCD841 cells to determine whether IRE1␣ could affect the cytotoxic activity of LPS.…”

Section: Iec-specific Ablation Of Ire1␣ Results In Spontaneous Colitismentioning

confidence: 99%

The Endoplasmic Reticulum Stress Sensor IRE1α in Intestinal Epithelial Cells Is Essential for Protecting against Colitis

Zhang

Chen

Фан

et al. 2015

Journal of Biological Chemistry

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Background: Endoplasmic reticulum (ER) stress is implicated in inflammatory bowel disease (IBD) and IRE1␣ plays a critical role in ER stress. Results: Genetic ablation of Ire1␣ in intestinal epithelial cells leads to colitis in mice. Conclusion: IRE1␣ acts as an important defense molecule against IBD. Significance: The finding provides insight into the regulation of intestinal epithelium homeostasis by IRE1␣.

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“…These studies have indicated that the terminal ileum is the major site for observation of damage to gut barrier functions following exposure to various injuries. It is suggested that terminal ileum is the most sensitive section of the intestinal tract [9]. …”

Section: Methodsmentioning

confidence: 99%

“…The intestinal permeability was also determined by calculating intestinal clearance of fluorescein-isothiocyanate dextran (FD4) as described in the literature [9, 10]. Briefly, a segment of terminal ileum with length of 8 cm was prepared, and the mucosa was everted.…”

Section: Methodsmentioning

confidence: 99%

The Effect of Peritoneal Air Exposure on Intestinal Mucosal Barrier

Bao

Tan

et al. 2014

Gastroenterology Research and Practice

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Background. Damage of the intestinal mucosa barrier may result in intestinal bacterial and endotoxin translocation, leading to local and systemic inflammation. The present study was designed to investigate whether peritoneal air exposure induces damage of intestinal mucosal barrier. Methods. Sprague-Dawley rats (weighing 210 to 230 g) were randomized into five groups (6/group): a control group, a sham group, and three exposure groups with peritoneal air exposure for 1, 2, and 3 h, respectively. At 24 h after surgery, blood and terminal ileum were sampled. The serum D-lactate levels were determined using an ELISA kit. The intestinal permeability was determined by measuring the intestinal clearance of FITC-dextran (FD4). The histopathological changes in terminal ileum were also assessed. Results. Compared with the controls, peritoneal air exposure caused an increase in both serum D-lactate level and intestinal FD4 clearance, which were proportional to the length of peritoneal air exposure and correlated to Chiu's scores, indices for intestinal mucosal injury. Edema and inflammatory cells were also observed in mucosa and submucosa of ileum in three exposure groups. Conclusions. Peritoneal air exposure could induce damage to the intestinal mucosal barrier, which is proportional to the time length of peritoneal air exposure.

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Lipopolysaccharide-induced failure of the gut barrier is site-specific and inhibitable by growth hormone

Abstract: Endotoxemia can induce gut barrier failure in rats, and the ileum is the site of greatest risk. The GH can reduce the incidence of endotoxemia-induced gut barrier failure, but not the associated mortality.

Cited by 25 publications

References 38 publications

Berberine Ameliorates Intestinal Mucosal Barrier Damage Induced by Peritoneal Air Exposure

Berberine Ameliorates Intestinal Mucosal Barrier Damage Induced by Peritoneal Air Exposure

The Endoplasmic Reticulum Stress Sensor IRE1α in Intestinal Epithelial Cells Is Essential for Protecting against Colitis

The Effect of Peritoneal Air Exposure on Intestinal Mucosal Barrier

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