Lipopolysaccharide (LPS) suppresses follicle development marker expression and enhances cytokine expressions, which results in fail to granulosa cell proliferation in developing follicle in cows

Yamamoto, Naoki; Takeuchi, Himeno; Yamaoka, Manami; Nakanishi, Tomoya; Tonai, Shingo; Nishimura, Ryo; Morita, Takehito; Nagano, Masashi; Kameda, Shingo; Genda, Kaori; Kawase, Jun; Yamashita, Yugo

doi:10.1016/j.repbio.2022.100710

Cited by 6 publications

(3 citation statements)

References 46 publications

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“…Studies have shown that BMPR1B regulates the differentiation of granulosa cells and follicle development by participating in the signal transduction of BMP2, BMP4, BMP6, BMP7, BMP15, and GDF5, thus affecting the reproductive cycle of mammals [37,38]. In particular, CYP19A1 can convert androgens into estrogen, and estrogen can inhibit apoptosis in granulosa cells and promote its differentiation by increasing the expression of LHCGR, the LH receptor, thus regulating follicle development [39,40]. In this study, the expression level of CYP19A1 was significantly higher during estrus compared to diestrus, with an increased change in plasma LH levels and a decrease in foraging and drinking times.…”

Section: Discussionmentioning

confidence: 99%

Behavioral and Physiological Differences in Female Rabbits at Different Stages of the Estrous Cycle

Chen,

Jin,

Yang

et al. 2023

Animals

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Estrus involves a series of complex physiological signs and changes in behavior before ovulation, which play a crucial role in animal reproduction. However, there have been few studies that evaluate behaviors during the different stages of estrus cycle in female rabbits. Therefore, more detailed information is needed on distinguishing the various stages of the estrous cycle. This study explored the behavioral and physiological differences at various estrous cycle stages in female New Zealand White rabbits. The continuous recording method was employed to record the daily behaviors of twenty postpartum female rabbits during the estrous cycle. Compared with the diestrus stage, the duration of foraging and drinking behavior in estrus decreased significantly, and the frequency of grooming and biting behaviors increased (p < 0.05). Differences in reproductive hormone levels (FSH, LH, P4, and E2) and follicle development were measured at each stage via ELISA and HE staining. The FSH and LH levels showed an increasing trend and then decreased, with the lowest being in late estrus (p < 0.05). The P4 level was the lowest in estrus (p < 0.05), and E2 showed a gradually increasing trend. There was no significant difference in the number of primordial follicles at each stage, but the number of primary follicles in estrus was significantly higher than at the other stages (p < 0.05). To further understand the molecular regulation mechanism of the estrous cycle in female rabbits, we analyzed the ovarian transcription patterns of female rabbits in diestrus (D group) and estrus (E group) employing RNA-seq. A total of 967 differentially expressed genes (DEGs) were screened from the ovaries of female rabbits between the diestrus and estrus groups. A KEGG analysis of DEGs enriched in the estrogen signaling pathway, aldosterone synthesis, and secretion pathway, such as CYP19A1 and IGF1R, was performed. The rabbits’ behavior, related physiological hormones, and molecular regulation also differed at different estrous cycle stages. The results provide recommendations for the adequate management practices of postpartum re-estrus and breeding female rabbits.

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Section: Discussionmentioning

confidence: 99%

Behavioral and Physiological Differences in Female Rabbits at Different Stages of the Estrous Cycle

Chen,

Jin,

Yang

et al. 2023

Animals

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“…Ç‚Â‰ÂÌËÂ. ùÌ‰ÓÏÂÚËÚ˚ Û ÌÓ‚ÓÚÂÎ¸Ì˚ı ÍÓÓ‚ -˝ÚÓ  ‡ÒÔÓÒÚ ‡ÌÂÌÌ ‡fl Ô ‡ÚÓÎÓ"Ëfl ÂÔÓ‰ÛÍÚË‚-ÌÓÈ ÒËÒÚÂÏ˚, ÍÓÚÓ ‡fl ÏÓÊÂÚ ÔË‚Ó‰ËÚ¸ Í Ì ‡Û-¯ÂÌË˛ ÙÂÚËÎ¸ÌÓÒÚË ÊË‚ÓÚÌ˚ı Ë, Í ‡Í ÒÎÂ‰-ÒÚ‚ËÂ, Í ˝ÍÓÌÓÏË˜ÂÒÍËÏ Û•˚ÚÍ ‡Ï ÔÓËÁ‚Ó‰ËÚÂÎÂÈ [1][2][3][4]. ÑËÒÙÛÌÍˆËfl flË˜ÌËÍÓ‚, ËÌ"Ë•ËÓ‚ ‡ÌËÂ  ‡Á‚ËÚËfl ÙÓÎÎËÍÛÎÓ‚ Ë Ó‚ÛÎflˆËË Ò‚flÁ ‡Ì˚ Ò ‰ÂÈ-ÒÚ‚ËÂÏ  ‡ÁÎË˜Ì˚ı Ô ‡ÚÓ"ÂÌÓ‚, ÍÓÚÓ˚Â ‚˚fl‚Îfl˛Ú-Òfl ÔË ˝Ì‰ÓÏÂÚËÚ ‡ı.…”

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“…all (2023) ÔÓÍ ‡Á ‡ÎË, ˜ÚÓ Toll-ÔÓ‰Ó•Ì˚Â ÂˆÂÔÚÓ˚ " ‡ÌÛÎÂÁÌ˚ı ÍÎÂÚÓÍ ‚Á ‡Ë-ÏÓ‰ÂÈÒÚ‚Û˛Ú Ò ÎËÔÓÔÓÎËÒ ‡ı ‡Ë‰ ‡ÏË " ‡ÏÓÚËˆ ‡-ÚÂÎ¸Ì˚ı • ‡ÍÚÂËÈ, ‚ ˜ ‡ÒÚÌÓÒÚË E. coli, ˜ÚÓ ÔË‚Ó‰ËÚ Í ‚ÓÒÔ ‡ÎËÚÂÎ¸ÌÓÈ Â ‡ÍˆËË Ë ‚˚ ‡•ÓÚÍÂ ˆËÚÓÍËÌÓ‚, Ú ‡ÍËı Í ‡Í Í ‡Í Ù ‡ÍÚÓ ÌÂÍÓÁ ‡ ÓÔÛ-ıÓÎË-α (TNF-α) Ë ËÌÚÂÎÂÈÍËÌ˚. Å˚ÎÓ ‚˚fl‚ÎÂ-ÌÓ, ˜ÚÓ ÍÓÓ‚˚ Ò ÍÎËÌË˜ÂÒÍËÏ ˝Ì‰ÓÏÂÚËÚÓÏ ËÏÂÎË ÒÌËÊÂÌÌÛ˛ ˝ÍÒÔÂÒÒË˛ Ï ‡ÍÂÓ‚  ‡Á‚Ë-ÚËfl ÙÓÎÎËÍÛÎÓ‚ ‚ ÍÎÂÚÍ ‡ı " ‡ÌÛÎÂÁ˚, •ÓÎÂÂ ÌËÁ-ÍÛ˛ ÍÓÌˆÂÌÚ ‡ˆË˛ ˝ÒÚ ‡‰ËÓÎ ‡-17β ‚ ÙÓÎÎËÍÛ-ÎflÌÓÈ ÊË‰ÍÓÒÚË, ‡ Ú ‡ÍÊÂ ÔÓÌËÊÂÌÌÛ˛ ÔÓÎËÙÂ ‡ˆË˛ ÍÎÂÚÓÍ " ‡ÌÛÎÂÁ˚, ˜ÚÓ, ÔÓ ÏÌÂ-ÌË˛ ‡‚ÚÓÓ‚, ÔË‚Ó‰ËÚ Í flÎÓ‚ÓÒÚË ÍÓÓ‚ [4]. èË ÎÂ˜ÂÌËË ÍÓÓ‚ Ò ˝Ì‰ÓÏÂÚËÚÓÏ ÏÌÓ"ËÂ ÒÔÂˆË ‡ÎËÒÚ˚ ÔËÏÂÌfl˛Ú  ‡ÁÎË˜Ì˚Â ÒÔÓÒÓ•˚ ‚‚Â-‰ÂÌËfl  ‡ÒÚ‚ÓÓ‚ ÌÓ‚ÓÍ ‡ËÌ ‡ Ò Û˜ÂÚÓÏ Â"Ó ‡ÌÂÒÚÂ-ÁËÓÎÓ"Ë˜ÂÒÍÓ"Ó Ë ÚÂ ‡ÔÂ‚ÚË˜ÂÒÍÓ"Ó ‰ÂÈÒÚ‚Ëfl.…”

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Assessment of complex treatment schemes for cows with postpartum endometritis and the efficiency of their subsequent insemination

Finageev,

Kuznetsova

2024

jour

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Purpose: сomparing the effectiveness of the proposed protocols for the treatment of cows with acute postpartum endometritis and establishing the effectiveness and the terms of their subsequent insemination.Materials and methods. 47 Cows of the Holstein-Frisan breed, which are on the binding content, were examined. All these animals were provided with obstetric care. As a result of their clinical inspection, 16 goals with an acute form of postpartum endometritis were revealed. Of these animals, two groups of 8 goals formed in each on the principle of pairs of analogues. When making a diagnosis, anamnesis, clinical signs of the disease and the results of an ultrasound of the study were taken into account. The first group included animals whose treatment regimen included intrauterine administration of Tilocar and intramuscular Uteroton. In the second group of cows, a 0.5 % Novocaine solution was additionally injected intravenously, according to the instructions. Results. The study revealed that the animals in the second group recovered three days faster (11.13±0.39 days) than the first group (14.25±0.37 days). We used the odds ratio. The second group of cows, where an additional 0.5 % of the Novocaine solution was administered, was found to be 2.78 times more likely to become pregnant than those treated without it.

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Potential mechanisms and therapeutic strategies for LPS-associated female fertility decline

Qin,

Du,

et al. 2024

J Assist Reprod Genet

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Lipopolysaccharide (LPS) suppresses follicle development marker expression and enhances cytokine expressions, which results in fail to granulosa cell proliferation in developing follicle in cows

Cited by 6 publications

References 46 publications

Behavioral and Physiological Differences in Female Rabbits at Different Stages of the Estrous Cycle

Behavioral and Physiological Differences in Female Rabbits at Different Stages of the Estrous Cycle

Assessment of complex treatment schemes for cows with postpartum endometritis and the efficiency of their subsequent insemination

Potential mechanisms and therapeutic strategies for LPS-associated female fertility decline

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