Lipopolysaccharide microarrays for the detection of antibodies

Thirumalapura, Nagaraja; Morton, Robert; Ramachandran, A.; Malayer, Jerry R.

doi:10.1016/j.jim.2005.01.004

Cited by 48 publications

(41 citation statements)

References 29 publications

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“…However, different and incompatible surface chemistries are required for different classes of biomolecules, and not all are compatible with device fabrication. Antibodies are immobilized onto aldehyde, epoxy, maleimide, or hydrophobic solid supports (68)(69)(70)(71). Variables such as pH, ionic strength, hydration, etc., must be controlled to prevent protein denaturation.…”

Section: Multiparameter Measurementsmentioning

confidence: 99%

Nanotechnology and Cancer

Heath

Davis²

2008

Annu. Rev. Med.

341

245

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The biological picture of cancer is rapidly advancing from models built from phenomenological descriptions to network models derived from systems biology, which can capture the evolving pathophysiology of the disease at the molecular level. The translation of this (still academic) picture into a clinically relevant framework can be enabling for the war on cancer, but it is a scientific and technological challenge. In this review, we discuss emerging in vitro diagnostic technologies and therapeutic approaches that are being developed to handle this challenge. Our discussion of in vitro diagnostics is guided by the theme of making large numbers of measurements accurately, sensitively, and at very low cost. We discuss diagnostic approaches based on microfluidics and nanotechnology. We then review the current state of the art of nanoparticle-based therapeutics that have reached the clinic. The goal of the presentation is to identify nanotherapeutic strategies that are designed to increase efficacy while simultaneously minimizing the toxic side effects commonly associated with cancer chemotherapies.

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Section: Multiparameter Measurementsmentioning

confidence: 99%

Nanotechnology and Cancer

Heath

Davis²

2008

Annu. Rev. Med.

341

245

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“…In some experiments FITC-conjugated goat anti-human C4 from MP Biomedicals was used at 1/2000 dilution. Labeling with fluorescent Abs was conducted 5 The online version of this article contains supplemental material. …”

Section: Microarray Production and Measurementsmentioning

confidence: 99%

Detection of Complement Activation on Antigen Microarrays Generates Functional Antibody Profiles and Helps Characterization of Disease-Associated Changes of the Antibody Repertoire

Papp

Végh

Miklós

et al. 2008

The Journal of Immunology

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Humoral immune responses are traditionally characterized by determining the presence and quality of Abs specific for certain Ags. Arraying of large numbers of Ags allows the parallel measurement of Abs, generating patterns called Ab profiles. Functional characterization of these Abs could help draw an even more informative map of an immune response. To generate functional Ab profiles we simultaneously tested not only IgM, IgG, and IgA binding to, but also complement activation by, a panel of endogenous and exogenous Ags printed as microarrays, using normal and autoimmune human sera. We show that complement activation by a particular Ag in a given individual cannot be predicted by the measurement of Ag-specific Abs, despite a general correlation between the amount of Ag-bound Ab and the deposited C3 fragments. This is due to both differences in the isotypes that dominate in the recognition of an Ag and individual variations for a given isotype, resulting in altered complement activation potential. Thus, Ag-specific C3 deposition can be used as an additional parameter in immune response monitoring. This is exemplified by comparing the coordinates of Ags, used for the diagnosis of systemic lupus erythematosus, of normal and autoimmune serum samples in a two-dimensional space derived from C3 deposition and Ab binding. Since cleavage fragments of C3 mediate important immunological processes, we propose that measurement of their deposition on Ag microarrays, in addition to Ab profiling, can provide useful functional signature about the tested serum.

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“…On the other side, gene microarrays (some authors call them gene chips) are devices enabling to recognize specific sequences through collected nucleic acids spots immobilized on an optical slide. Antibody microarray based on lipopolysaccharides immobilized on the nitrocellulose-coated glass slides was presented for antibodies against Escherichia coli, Salmonella typhimurium, and F. tularensis with the limit of detection of 10 ng/ml (Thirumalapura et al, 2005).…”

Section: Biosensorsmentioning

confidence: 99%

Current and emerging assays for Francisella tularensis detection: a review

Pohanka¹,

Hubálek²,

Neubauerová³

et al. 2008

Vet. Med. - Czech

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This paper presents an overview of methods for detection and identification of the pathogenic bacterium Francisella tularensis such as cultivation tests, enzyme-linked immunosorbent assays, flow cytometry, polymerase chain reaction, immunosensor, microarray, mass spectrometry, and chromatography. Included references are chosen according to their practical importance or perspectives for the future.

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Lipopolysaccharide microarrays for the detection of antibodies

Cited by 48 publications

References 29 publications

Nanotechnology and Cancer

Nanotechnology and Cancer

Detection of Complement Activation on Antigen Microarrays Generates Functional Antibody Profiles and Helps Characterization of Disease-Associated Changes of the Antibody Repertoire

Current and emerging assays for Francisella tularensis detection: a review

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