Lipopolysaccharide-preconditioned allogeneic adipose-derived stem cells improve erectile function in a rat model of bilateral cavernous nerve injury

Abstract

Background Erectile dysfunction (ED) often occurs due to cavernous nerve injury (CNI) after colorectal surgery. Cell-based therapies have great potential for the treatment of CNI-related ED; however, it needs to be optimised. In this study, we explored the therapeutic effects of lipopolysaccharide-preconditioned allogeneic adipose-derived stem cells (L-ADSCs) on CNI-induced ED in rats. Results The results of this in vitro study revealed that low-dose lipopolysaccharide could increase the viability of ADSCs, inhibit caspase 3 activation induced by hydrogen peroxide and promote cell migration. Compared with the ADSC supernatant, the L-ADSC supernatant could better reduce fibrosis in the corpus cavernosum smooth muscle cells induced by transforming growth factor-beta 1 protein. In the in vivo study, it was compared to ADSCs therapy, where the L-ADSCs therapy indicated that could better improve erectile function by increasing smooth muscle content and alleviating penile fibrosis in rats 2 weeks after CNI. The outcome may be related to the increase in the hepatocyte growth factor content in the corpus cavernosum and myelin basic protein in the major pelvic ganglion. Conclusions L-ADSC treatment may be a promising approach for restoring erectile function after CNI.

Overexpression of PRDX2 in Adipose-Derived Mesenchymal Stem Cells Enhances the Therapeutic Effect in a Neurogenic Erectile Dysfunction Rat Model by Inhibiting Ferroptosis

“…In the current study, ADSCs could improve the fibrosis of CCSMC, similar to the findings of several studies [ 42 , 43 , 47 ]. BCNI can cause irreversible progression of penis tissue fibrosis through the deposition of extracellular matrix (ECM), which occurs in the early stage after injury [ 43 ].…”

“…In the current study, ADSCs could improve the fibrosis of CCSMC, similar to the findings of several studies [ 42 , 43 , 47 ]. BCNI can cause irreversible progression of penis tissue fibrosis through the deposition of extracellular matrix (ECM), which occurs in the early stage after injury [ 43 ]. Zhang et al and Wu et al reported that MSCs derived from human gingiva or ADSCs pretreated with lipopolysaccharide could inhibit the expression of ECM (collagen I/IV and fibronectin) proteins in the corpus cavernosum of BCNI rats, thereby improving penile fibrosis [ 43 , 48 ].…”

“…It is a disease that is difficult to cure, and most traditional treatment methods show limited effectiveness [ 40 , 41 ]. Stem cell therapy for NED is a promising therapeutic method [ 29 , 42 , 43 ]. However, it needs to be optimized prior to clinical application due to its low survival rate and limited therapeutic effect in the transplanted OS microenvironment.…”

“…BCNI can cause irreversible progression of penis tissue fibrosis through the deposition of extracellular matrix (ECM), which occurs in the early stage after injury [ 43 ]. Zhang et al and Wu et al reported that MSCs derived from human gingiva or ADSCs pretreated with lipopolysaccharide could inhibit the expression of ECM (collagen I/IV and fibronectin) proteins in the corpus cavernosum of BCNI rats, thereby improving penile fibrosis [ 43 , 48 ]. In our study, Masson's trichrome staining showed that ADSCs and PRDX2-ADSCs have significant therapeutic effects on NED.…”

Therapies Based on Adipose-Derived Stem Cells for Lower Urinary Tract Dysfunction: A Narrative Review

“…Lipopolysaccharides can be recognized by Toll-like receptors, and Toll-like receptors can be expressed on the surface of ASCs [ 75 , 76 ]. Thus, Zhang et al [ 77 ] pre-treated allogenic ASCs with lipopolysaccharides for the treatment of ED caused by cavernous nerve injury. Low doses of lipopolysaccharides can effectively improve the survival rate of ASCs and promote cell migration in vitro and inhibit caspase-3 activation induced by hydrogen peroxide, as well as downregulating TGF-β1 expression levels to delay smooth muscle fibrosis in vivo.…”