“…The proteins were transferred onto polyvinylidenedifluoride (PVDF) membranes (Millipore, Belford, MA), which were subsequently hybridized with the primary antibodies against Chk1, Chk2, CDC25C, cyclin A and B, CDK1 and 2, ATF‐6α, ATF‐6β, GADD153, GRP78, P‐PERK, Calpain 1 and 2, Caspase‐12, ‐3, ‐4, ‐8, and ‐9, DR4 and 5, TRAIL, Bax, Bid, Bcl‐2, MCL‐1, AIF, Endo G, XIAP, cytochrome c, and β‐actin followed by secondary antibodies. Protein bands were detected using the enhanced chemiluminescence using ECL detection (Millipore, Temecula, CA, USA) …”