Lipopolysaccharides affect compressed periodontal ligament cells via Eph–ephrin signaling

Li, Minjie; Tang, Zhongyuan; Zhang, Chengfei; Jin, Lijian; Matsuo, Koichi; Yang, Yanqi

doi:10.1111/odi.13875

Cited by 8 publications

(3 citation statements)

References 64 publications

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“…Previous study had shown that orthodontic movement of teeth was possible with minimal loss of alveolar bone. [40] During the orthodontic molar uprighting, alveolar bone defect could be repaired, and pocket depth and the crown to root ratio were observed to improve as well. [41] This was good news to us as we planned to have mandibular second premolars extracted and uprighted molars.…”

Section: Discussionmentioning

confidence: 99%

Nonsurgical treatment for an adult with open bite, narrow upper arch, and several impacted premolars

Weng,

Jiang,

et al. 2023

Preprint

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Anterior open bite refers to the opposing incisors do not come into contact. Skeletal class II malocclusion is characterized by maxillary protrusion, mandibular retrusion, or both. These two types of malocclusion can affect the patient’s facial appearance, masticatory function and mental health. They are big challenges to orthodontists and orthognathic surgery is usually needed to solve these problems. This case report describes an adult patient with the facial-skeletal problem of anterior open bite, narrow upper arch and skeletal Class II malocclusion, and malocclusion of severe dentition crowding, impacted premolars and posterior crossbite, meanwhile with periodontitis (stages III, grade B). The multidisciplinary management included teeth removing, maxillary skeletal expansion, posterior teeth intrusion, en masse retraction of upper anterior teeth and without orthognathic surgery. The esthetic facial profile and proper occlusal relationship were obtained.

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Section: Discussionmentioning

confidence: 99%

Nonsurgical treatment for an adult with open bite, narrow upper arch, and several impacted premolars

Weng,

Jiang,

et al. 2023

Preprint

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“…The primer sequences synthesized by Tsingke Biotechnology Co., Ltd. are shown in Table 1 . The qPCR data of the above coding genes and miR-210 were normalized with the expression of GAPDH and U6 as internal controls, and the resulting data were analyzed by the 2 −ΔΔCt method [ 27 ].…”

Section: Methodsmentioning

confidence: 99%

MiR-210 promotes bone formation in ovariectomized rats by regulating osteogenic/adipogenic differentiation of bone marrow mesenchymal stem cells through downregulation of EPHA2

Ren,

Zhu,

Tan

et al. 2023

J Orthop Surg Res

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Purpose In osteoporosis, the balance between osteogenic and adipogenic differentiation of mesenchymal stem cells (MSCs) is disrupted. The osteogenic differentiation of bone marrow MSCs (BMSCs) is important for improving osteoporosis. The aim of this study was to explore the role and molecular mechanism of miR-210 in the balance of osteogenic/adipogenic differentiation of BMSCs in postmenopausal osteoporosis. Methods Postmenopausal osteoporosis rat models were constructed by ovariectomy (OVX). BMSCs were isolated from the femur in rats of Sham and OVX groups. MiR-210 was overexpressed and suppressed by miR-210 mimics and inhibitor, respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the relative mRNA expression of miR-210, ephrin type-A receptor 2 (EPHA2), alkaline phosphatase (ALP), osterix (OSX), osteocalcin (Bglap), Runt-related transcription factor 2 (Runx2), peroxisome proliferator activated receptor gamma, and fatty acid binding protein 4 (FABP4) in each group of rat femoral tissues or BMSCs. Western blot was applied to detect the protein expression level of EPHA2 in rat femoral tissues and cells. Alizarin red S staining and oil red O staining were performed to assess the osteogenic and adipogenic differentiation of BMSCs, respectively. In addition, the targeting relationship between miR-210 and EPHA2 was verified by a dual luciferase gene reporter assay. Results The expression of miR-210 was significantly reduced in femoral tissues and BMSCs of OVX rats, and its low expression was associated with reduced bone formation. The osteogenic differentiation was enhanced in OVX rats treated with miR-210 mimic. Overexpression of miR-210 in transfected BMSCs was also found to significantly promote osteogenic differentiation and even inhibit adipogenic differentiation in BMSCs, while knockdown of miR-210 did the opposite. Further mechanistic studies showed that miR-210 could target and inhibit the expression of EPHA2 in BMSCs, thus promoting osteogenic differentiation and inhibiting adipogenic differentiation of BMSCs. Conclusion MiR-210 promotes osteogenic differentiation and inhibits adipogenic differentiation of BMSCs by down-regulating EPHA2 expression. As it plays an important role in the osteogenic/adipogenic differentiation of osteoporosis, miR-210 can serve as a potential miRNA biomarker for osteoporosis.

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“…Lipopolysaccharide (LPS) that constitutes the outer leaflet of the outer membrane of most Gram-negative bacteria participates in the immune responses of periodontitis [ 11 , 12 ]. Li et al reported that treatment with LPS and CF critically enhanced osteoclastogenesis in PDLSCs through the suppression of EphB4 and the induction of ephrinA2 signaling [ 13 ]. AlQranei et al reported that TNF α secreted via LPS/TLR4 signaling regulated osteoclastogenesis in macrophages primed with RANKL and then treated with LPS [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

EZH2 Regulates Lipopolysaccharide-Induced Periodontal Ligament Stem Cell Proliferation and Osteogenesis through TLR4/MyD88/NF-κB Pathway

Wang

Tian

Zhang

et al. 2021

Stem Cells International

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Background. Periodontitis induced by bacteria especially Gram-negative bacteria is the most prevalent chronic inflammatory disease worldwide. Emerging evidence supported that EZH2 plays a significant role in the inflammatory response of periodontal tissues. However, little information is available regarding the underlying mechanism of EZH2 in periodontitis. This study is aimed at determining the potential role and underlying mechanism of EZH2 in periodontitis. Methods. The protein levels of EZH2, H3K27ME, p-p65, p-IKB, TLR4, MyD88, Runx2, and OCN were examined by western blot assay. Proliferation was evaluated by CCK8 assay. The levels of TNFα, IL1β, and IL6 were detected by ELISA assay. Migration was detected by wound healing assay. The distribution of p65 was detected by immunofluorescence. The formation of mineralized nodules was analyzed using alizarin red staining. Results. LPS stimulation significantly promoted EZH2 and H3K27me3 expression in primary human periodontal ligament stem cells (PDLSCs). Targeting EZH2 prevented LPS-induced upregulation of the inflammatory cytokines and inhibition of cell proliferation and migration. Furthermore, EZH2 knockdown attenuated the TLR4/MyD88/NF-κB signaling to facilitate PDLSC osteogenesis. Conclusions. Modulation of the NF-κB pathway through the inhibition of EZH2 may offer a new perspective on the treatment of chronic apical periodontitis.

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Lipopolysaccharides affect compressed periodontal ligament cells via Eph–ephrin signaling

Cited by 8 publications

References 64 publications

Nonsurgical treatment for an adult with open bite, narrow upper arch, and several impacted premolars

Nonsurgical treatment for an adult with open bite, narrow upper arch, and several impacted premolars

MiR-210 promotes bone formation in ovariectomized rats by regulating osteogenic/adipogenic differentiation of bone marrow mesenchymal stem cells through downregulation of EPHA2

EZH2 Regulates Lipopolysaccharide-Induced Periodontal Ligament Stem Cell Proliferation and Osteogenesis through TLR4/MyD88/NF-κB Pathway

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