Lipopolysaccharides, cytokines, and nitric oxide affect secretion of prostaglandins and leukotrienes by bovine mammary gland during experimentally induced mastitis in vivo and in vitro

Piotrowska-Tomala, K.K.; Bah, Mamadou M.; Jankowska, K; Łukasik, Karolina; Warmowski, P; Galvão, António; Skarzynski, Dariusz J.

doi:10.1016/j.domaniend.2015.03.001

Cited by 19 publications

(8 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among cows with a CL at the time of treatment with LPS, however, there was no effect of LPS intrauterine infusion on P4 concentration. Piotrowska-Tomala et al (2015) demonstrated that in vitro and in vivo challenges with LPS stimulated the secretion of PGE 2 , PGF 2α , and 13,14-dihydro,15-keto-PGF 2α by the bovine mammary gland. Lima et al (2015) demonstrated that 12 h after intrauterine treatment with TNF-α and Trueperella pyogenes there was an increase in the expression of mRNA for oxytocin receptor in the endometrium.…”

Section: Discussionmentioning

confidence: 96%

Effects of intrauterine infusion of Escherichia coli lipopolysaccharide on uterine mRNA gene expression and peripheral polymorphonuclear leukocytes in Jersey cows diagnosed with purulent vaginal discharge

Moraes

Mendonça

Silva

et al. 2017

Journal of Dairy Science

View full text Add to dashboard Cite

The objectives of the current experiment were to investigate the effects of intrauterine treatment of cows with purulent vaginal discharge (PVD) using lipopolysaccharide (LPS) from Escherichia coli on uterine mRNA expression of genes related to inflammatory responses, peripheral polymorphonuclear leukocyte (PMN) function, hematological parameters, and blood concentrations of cortisol, haptoglobin, and progesterone (P4). Jersey cows (n = 3,084) were examined for PVD at 35 ± 6 d postpartum using the Metricheck device (Simcro, Hamilton, New Zealand). At examination, 310 cows had PVD (10.1%), but to ensure proper collection and processing of samples, 267 cows were used in this experiment. Cows were balanced for lactation number, body condition score, and milk yield, and randomly assigned to the control treatment [intrauterine infusion of 20 mL of phosphate-buffered saline (PBS); n = 87] or to receive intrauterine infusion of 20 mL of PBS containing 150 µg (LPS150; n = 91) or 300 µg (LPS300; n = 89) of E. coli LPS. Uteri were biopsied in a subgroup of cows at 6 h after infusion and in another subgroup of cows at 24 h after infusion. Peripheral PMN expression of adhesion molecules (L-selectin and MAC-1) and phagocytosis and oxidative burst were evaluated at 0, 2, and 6 h after infusion. Blood sampled 0, 2, 6, 24, 48, and 168 h after infusion was used for complete hemogram and to determine concentrations of cortisol, haptoglobin, and P4. Treatment did not affect uterine mRNA expression of adhesion molecules [endothelial leukocyte adhesion molecule (E-selectin), intracellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1)], cytokines (tumor necrosis factor-α, IL-1β, IL-6, IL-8, and IL-10), and toll-like receptor-4. Treatment did not affect PMN expression of L-selectin, but intensity of expression of MAC-1 was higher for LPS150 cows than PBS cows, and tended to be higher in LPS150 than LPS300 cows. Furthermore, a greater percentage of PMN from LPS300 cows were positive for phagocytosis and oxidative burst compared with PBS and LPS150 cows. No effects were observed of treatment on hematological parameters and concentrations of cortisol, haptoglobin, and P4. These observations suggest that intrauterine infusion of E. coli LPS moderately stimulates peripheral PMN function, but further research is needed to better understand the immunomodulatory effects of LPS in the uterus of cows with PVD.

show abstract

Section: Discussionmentioning

confidence: 96%

Effects of intrauterine infusion of Escherichia coli lipopolysaccharide on uterine mRNA gene expression and peripheral polymorphonuclear leukocytes in Jersey cows diagnosed with purulent vaginal discharge

Moraes

Mendonça

Silva

et al. 2017

Journal of Dairy Science

View full text Add to dashboard Cite

show abstract

“…In mastitis, activated Toll-like Receptors initiated the NF-κB/MAPKs pathways, which further triggered the gene expression of TNF-α, IL-1β and IL-6 [23]. Tomala et al [24] showed that LPS, IL-1α and TNF-α modulated PGE2 and LTB4 secreted by bovine mammary gland in both vivo and vitro studies. In agreement with the foregoing studies, we found that the serum levels of PGE2, LTB4, IL-1, IL-2, IL-6, IL-8 and TNF-α in clinical mastitis cows were signi cantly higher than healthy cows.…”

Section: Discussionmentioning

confidence: 99%

Effect of a natural plant ingredient — Pulsatilla saponin B4 on clinical mastitis and serum inflammatory indices in dairy cows

Shen

Qian

You

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Bovine mastitis is an inflammatory reaction of the breast caused by pathogenic bacteria infection or breast injury, which affect milk yield and quality and poses severe threat to the health of dairy cows. Pulsatilla chinensis has antibacterial, anti-inflammatory, and immune enhancing effects, etc. However, there was no research about the therapeutic effect of it towards the treatment of cow mastitis. In the study, twelve healthy cows were selected as the control group (group A), and 36 cows with mastitis were equally and randomly divided into group B, C and D. Group A didn’t accept treatment, while group B, C and D were treated with 15, 30 and 60 mL Pulsatilla saponin B4 (a purified extract form Pulsatilla chinensis) injection by brachiocephalicus intramuscular injection once a day for 4-6 day and the optimal dose was selected from them. Then, we investigated the effect of this extract on the clearance rates of pathogenic bacteria, the regulation of somatic cell count (SCC) and inflammatory factors the in appropriate dose group.Results: Pulsatilla saponin B4 was effective in all test groups and showed a dose-effect relationship. The extract had inhibitory effects on Streptococcus agalactiae, Streptococcus dysenteriae, Klebsiella species, Mycoplasma bovis, Escherichia coli and Staphylococcus aureus. Group C cured more cows than group B and D during the 6 days of treatment and was determined as the optimal dose group. The milk SCC, serum haptoglobin (HP), prostaglandin E2 (PGE2), interleukin-1 alpha (IL-1α), interleukin-1 beta (IL-1β), interleukin-2 (IL-2), interleukin-8 (IL-8), interleukin-10 (IL-10) and tumour necrosis factor alpha (TNF-α), interleukin-4 (IL-4) and leukotriene B4 (LTB4) in Group C were significantly higher than those of healthy cows (P < 0.05) on the first day but had no significant difference with group A after treatment. Conclusion: 30 mL Pulsatilla saponin B4 applied through intramuscular injection once a day for 4-6 days leaded to the significant amelioration of bacterial infection, SCC and levels of serum inflammatory factors in cows with clinical mastitis.

show abstract

“…The clinical condition of all cases improved on day 1 as a result of initial systemic administration of OTC, regardless of whether the bacterial strain was susceptible. Acute ACM symptoms appear as a result of an excessive inflammatory reaction to lipopolysaccharides, a structural component of the cell wall of gram-negative bacilli [15], and it has been shown that the severity of symptoms depends on the amount of lipopolysaccharides in the udder [6]. In addition, it has been reported that experimental injection of unmixed lipopolysaccharides into the udder can reproduce the same pathological conditions as ACM [23].…”

Section: Discussionmentioning

confidence: 99%

Influence of oxytetracycline susceptibility as a first-line antibiotic on the clinical outcome in dairy cattle with acute <i>Escherichia coli</i> mastitis

Shinozuka

Kawai

Takeda³

et al. 2019

J. Vet. Med. Sci.

View full text Add to dashboard Cite

The purpose of this study was to clarify the therapeutic effects of oxytetracycline (OTC) as a first-line antibiotic in cattle with acute Escherichia coli mastitis and systemic signs. Drug susceptibility was determined by the minimum inhibitory concentration (MIC) of E. coli isolated from cows with acute E. coli mastitis (n=38). Cattle were divided into OTC-susceptible (S, n=30) and OTC-resistant (R, n=8) groups. They were further subdivided according to susceptibility to the antibiotic used as a second treatment, into susceptible-susceptible (SS, n=30), resistant-susceptible (RS, n=5), and resistant-resistant (RR, n=3) groups. Clinical signs on the day after initial treatment were compared between S and R groups as short-term indicators of treatment effects. The 28-day survival rate of cattle was then compared among SS, RS, and RR groups as a long-term indicator of treatment effects. There were no differences in clinical signs between S and R groups on the day after the first dose, but the 28-day survival rate was significantly greater in the SS group than in the RR group ( P =0.04). The results demonstrated that an effective drug is essential for first-line treatment of acute coliform mastitis. However, anticipating the effectiveness of a first-line antibiotic based on clinical symptoms at the second day of treatment is impossible. It is important to build a picture of drug resistance trends in cattle herds for empirical selection of antibiotics to be administered.

show abstract

Lipopolysaccharides, cytokines, and nitric oxide affect secretion of prostaglandins and leukotrienes by bovine mammary gland during experimentally induced mastitis in vivo and in vitro

Cited by 19 publications

References 46 publications

Effects of intrauterine infusion of Escherichia coli lipopolysaccharide on uterine mRNA gene expression and peripheral polymorphonuclear leukocytes in Jersey cows diagnosed with purulent vaginal discharge

Effects of intrauterine infusion of Escherichia coli lipopolysaccharide on uterine mRNA gene expression and peripheral polymorphonuclear leukocytes in Jersey cows diagnosed with purulent vaginal discharge

Effect of a natural plant ingredient — Pulsatilla saponin B4 on clinical mastitis and serum inflammatory indices in dairy cows

Influence of oxytetracycline susceptibility as a first-line antibiotic on the clinical outcome in dairy cattle with acute <i>Escherichia coli</i> mastitis

Contact Info

Product

Resources

About