Lipoprotein capture ELISA method for the sensitive detection of amphiphilic biomarkers

Lenz, Kiersten D.; Klosterman, Katja E.; Mukundan, Harshini; Kubicek-Sutherland, Jessica Z.

doi:10.1016/j.ab.2022.114747

Cited by 5 publications

(4 citation statements)

References 34 publications

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“…This response indicates that amphiphilic PAMPs avoid immune stimulation by associating themselves with lipoproteins found in serum. We additionally showed that both LAM (62) and mycolactone can interact with any of the four major serum lipoprotein classes, indicating that this association is nonspecific and likely due to a combination of hydrophobic and hydrophilic interactions. Interestingly, the presence of both molecules is immunosuppressive with or without serum, indicating that the two molecules inhibit macrophages along different, but not unrelated, pathways.…”

Section: Discussionmentioning

confidence: 85%

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Serum lipoproteins and lipoarabinomannan suppress the inflammatory response induced by the mycolactone toxin

Jakhar¹,

Lenz²,

Jacobsen³

et al. 2022

Preprint

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Mycobacterium ulcerans is the causative agent of the chronic and debilitating neglected tropical disease Buruli ulcer (BU) that mostly affects children. The early detection and treatment of M. ulcerans infections can significantly minimize life-long disability resulting from surgical intervention. However, the disease is characterized by relatively few systemic symptoms due to complex host-pathogen interactions that have yet to be fully characterized, which has limited the development of both diagnostic and therapeutic approaches to treat BU. In this work, we study the interactions of the host immune system with two principle M. ulcerans virulence factors: mycolactone, an amphiphilic macrolide toxin, and lipoarabinomannan (LAM), a cell wall component of most mycobacterial pathogens. We observe that human lipoproteins have a profound effect on the interaction of both mycolactone and LAM with the immune system. Individually, both molecules are pro-inflammatory in the absence of serum and immunosuppressive in the presence of serum. When combined, mycolactone and LAM are immunosuppressive regardless of serum conditions. We also show that Toll-like receptor 2 (TLR2), a macrophage pathogen pattern recognition receptor, is critical for LAM immune stimulation but aids in mycolactone immunosuppression. These findings are a critical step towards unraveling mycolactone-mediated immunosuppression during BU disease and may facilitate the development of effective diagnostics and therapeutics in the future.

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Section: Discussionmentioning

confidence: 85%

“…We tested each of these lipoproteins' interaction with mycolactone at physiologically relevant concentrations (53,54,56,(59)(60)(61). We have previously described the interaction of LAM with serum lipoproteins (62).…”

Section: Mycolactone Directly Interacts With Serum Lipoproteinsmentioning

confidence: 99%

Serum lipoproteins and lipoarabinomannan suppress the inflammatory response induced by the mycolactone toxin

Jakhar¹,

Lenz²,

Jacobsen³

et al. 2022

Preprint

Self Cite

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“…Compared to previously published research (Table S2, ESI †), this strategy reached a lower detection limit, primarily due to the synergistic effect of dual stabilizers and selective recognition of probe-Cu 2+ . [35][36][37][38][39][40][41] Furthermore, its non-invasive sampling, homogeneity, and room temperature reaction make it highly clinically applicable in tuberculosis auxiliary diagnosis.…”

Section: Analytical Performance Of the Lam Assaymentioning

confidence: 99%

Fructose@histone synergistically improve the performance of DNA-templated Cu NPs: rapid analysis of LAM in tuberculosis urine samples using a handheld fluorometer and a smartphone RGB camera

Meng,

Wang,

Zhan

et al. 2024

J. Mater. Chem. B

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“…Day et al ( 39 ) performed ELISA to detect enterovirus-specific IgM in serum samples from 557 patients clinically diagnosed with meningitis or encephalitis and found that the detection rate was much higher than the virus isolation rate. Detection of EV IgM by ELISA is therefore a more sensitive, economical, and rapid diagnostic method than virus isolation and is not affected by viral viability ( 40 , 41 ). For some viruses, including Epstein–Barr virus (EBV), potentially associated with neurological diseases such as multiple sclerosis (MS), activation of a polyclonal response at the intrathecal level has been described ( 42 ).…”

Section: Etiological Examinationmentioning

confidence: 99%

Progress in etiological diagnosis of viral meningitis

Xu,

Chen,

Guo

et al. 2023

Front. Neurol.

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In recent years, with the rapid development of molecular biology techniques such as polymerase chain reaction and molecular biochip, the etiological diagnosis of viral encephalitis has a very big step forward. At present, the etiological examination of viral meningitis mainly includes virus isolation, serological detection and molecular biological nucleic acid detection. This article reviews the progress in etiological diagnosis of viral meningitis.

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Lipoprotein capture ELISA method for the sensitive detection of amphiphilic biomarkers

Cited by 5 publications

References 34 publications

Serum lipoproteins and lipoarabinomannan suppress the inflammatory response induced by the mycolactone toxin

Serum lipoproteins and lipoarabinomannan suppress the inflammatory response induced by the mycolactone toxin

Fructose@histone synergistically improve the performance of DNA-templated Cu NPs: rapid analysis of LAM in tuberculosis urine samples using a handheld fluorometer and a smartphone RGB camera

Progress in etiological diagnosis of viral meningitis

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