Lipoprotein lipase and hepatic lipase in Wistar and Sprague‐Dawley rat tissues. Differences in the effects of gender and fasting

Galan, Xavier; Llobera, Miquel; Ramı́rez, Ignasi

doi:10.1007/bf02537186

Cited by 53 publications

(30 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results for LPL activity obtained in the present study are in agreement with data from other studies on fasted rats [24,25]. Our results showed no effect of pregnancy on the LPL activity of subcutaneous or parametrial adipocytes.…”

Section: Discussionsupporting

confidence: 83%

Triglyceride Turnover, Lipoprotein Lipase Activity, and Fat Cell Size in Adipose Tissue of Rats during the First 2 Weeks of Pregnancy

Sohlström

Petterson²,

Forsum³

1998

Ann Nutr Metab

View full text Add to dashboard Cite

Understanding the biological function of the fat retained during pregnancy is important when estimating energy needs during reproduction. Women as well as rats are often considered to gain fat at specific anatomical sites during pregnancy and use this fat as a source of energy during lactation. However, mobilized body fat covers only a minor part of the energy needed by the lactating rat dam. In this paper, fat cell size, lipoprotein lipase activity, as well as triglyceride turnover in parametrial and subcutaneous adipose tissues were studied during the first 2 weeks of gestation and in virginal controls to further explore the metabolic and physiological basis for changes in body fat during reproduction in rats. Pregnancy increased the size of parametrial but not of subcutaneous adipocytes. The lipoprotein lipase activity in subcutaneous adipocytes was not increased by pregnancy. The accumulation in adipose tissue of ¹⁴C from orally administered ¹⁴C-oleic acid was higher in virginal than in pregnant rats. No effect of pregnancy on the rate of lipid turnover in parametrial or subcutaneous adipocytes was found. The findings are in accordance with the contention that body fat gained during rat pregnancy, to a large extent, is a consequence of a general growth of maternal tissues rather than the result of a stimulating effect of pregnancy on fat accumulation by adipocytes from specific body sites.

show abstract

Section: Discussionsupporting

confidence: 83%

Triglyceride Turnover, Lipoprotein Lipase Activity, and Fat Cell Size in Adipose Tissue of Rats during the First 2 Weeks of Pregnancy

Sohlström

Petterson²,

Forsum³

1998

Ann Nutr Metab

View full text Add to dashboard Cite

show abstract

“…Baseline respiratory rate (breaths per minute Ϯ SD) did not differ between groups (2 M: 78.9 Ϯ 8.2, saline: 88.6 Ϯ 14.4). (Galan et al 1994; LopezRubalcava and Lucki 2000;Pilz et al 1999;Shoaib et al 1995) and also that chemoreceptor responsiveness may be higher in Wistar-Kyoto rats than in rats of the Sprague-Dawley strain (Hayward et al 1999).In most clinical experiments, 10 ml of 0.5 M lactate solution have been given for a period of 20 min; the flow rate hence has been the same as in our animal experiments (0.5 ml/kg/min). Since the maximal respiratory response to lactate in rat was usually observed already within the first 5 min of infusion, it was decided to stop the infusion after 5 min 20 s; the total amount of lactate solution administered has hence been less in our animal experiments than in most clinical studies.…”

mentioning

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Respiratory Responses to Intravenous Infusion of Sodium Lactate in Male and Female Wistar Rats

Olsson

Annerbrink

et al. 2002

Neuropsychopharmacology

View full text Add to dashboard Cite

In patients with panic disorder or premenstrual dysphoria, anxiety attacks can be triggered by intravenous administration of sodium lactate. Since respiratory symptoms, such as hyperventilation and shortness of breath, are characteristic features of spontaneous as well as lactate-induced panic, an involvement of central or peripheral chemoreceptors in this reactionIn patients with panic disorder (Liebowitz et al. , 1985Pitts and McClure 1967) or premenstrual dysphoria (Facchinetti et al. 1992;Sandberg et al. 1993), but not in healthy controls, intravenous (i.v.) administration of sodium lactate elicits an anxiety reaction strongly resembling spontaneous panic attacks. The anxiogenic effect of sodium lactate has been attributed to changes in calcium concentrations (Pitts and McClure 1967), pH (Grosz andFarmer 1972), redox activity (Carr et al. 1986), pCO 2 (Gorman et al. 1988a), and osmolarity (Jensen et al. 1991;Peskind et al. 1998). However, all these theories and findings have also been questioned, and the mechanism by which sodium lactate elicits panic attacks hence is as yet unexplained.Likewise, the reason why patients with panic disorder or premenstrual dysphoria differ from controls with respect to sensitivity to lactate is a matter of controversy. Notably, a more rapid and substantial rise in brain lactate has been observed in panic patients as compared with control subjects (Dager et al. 1999), but the difference between patients and controls may also be due to different responsiveness to a given concentration (Pohl et al. 1994;Rifkin et al. 1981).Panic attacks are almost always associated with hyperventilation and respiratory discomfort, such as shortness of breath; it has thus been suggested that lactate-induced as well as spontaneous panic attacks may be due to an activation of a hyperresponsive suffocation alarm system involving activation of central or peripheral chemoreceptors (Klein 1993). Supporting this concept, an anxiety-provoking effect similar to that of lactate has been observed after inhalation of CO 2 in panic disorder subjects and in women with premenstrual dysphoria (Gorman et al. , 1988aGriez et al. 1987;Harrison et al. 1989;Kent et al. 2001;Papp et al. 1997;Perna et al. 1994;Woods et al. 1986).We have previously reported that intravenous administration of sodium lactate to Wistar rats, but not to rats of the Sprague-Dawley strain, elicits an increase in blood pressure and variable effects on heart rate (Wikander et al. 1995). Such cardiovascular response is in line with the assumption that lactate induces a stimulation of central or peripheral chemoreceptors. In the present study, the effect of sodium lactate on respiratory rate and tidal volume in freely moving, unrestrained male and female rats of the Wistar strain was explored. Given the previous reports suggesting that spontaneous panic attacks are menstrual cycle-dependent (Basoglu et al. 2000;Breier et al. 1986;Cameron et al. 1988;Cook et al. 1990;Klein 1993), that panic disorder may improve during pregnancy (Hertzberg and Wa...

show abstract

“…Differences in GDH mRNA content between hepatocytes in pericentral zones of males and females reflect a regulation mechanism at posttranscriptional and pretranslational levels. There have been previous reports of genderspecific expression patterns of mRNA and enzyme activity in rat liver (Furuya et al, 1994;Galan et al, 1994;Teutsch and Lowry, 1982;Wimmer and Pette, 1979). However, in these studies the level at which regulation existed could not be established.…”

Section: Discussionmentioning

confidence: 95%

Gender-dependent regulation of glutamate dehydrogenase expression in periportal and pericentral zones of rat liver lobules.

Geerts¹,

Verburg²,

Jonker³

et al. 1996

J Histochem Cytochem.

View full text Add to dashboard Cite

We studied the level(s) at which glutamate dehydrogenase (GDH; EC 1.4.1.2) expression is regulated in the livers of fed male and female rats. The cellular content of GDH mRNA, protein, and enzyme activity was determined quantitatively using image analysis for measnrement of the absorbance in consecutive serial sections that were processed for in situ hybridization, immunohistochemistry, and enzyme histochemistry. In both males and females, GDH protein and activity patterns were similar, with peci"al dues being twice as high as periportal values. GDH mRNA distribution patterns in female liver lobules reflected those of

show abstract

Lipoprotein lipase and hepatic lipase in Wistar and Sprague‐Dawley rat tissues. Differences in the effects of gender and fasting

Cited by 53 publications

References 48 publications

Triglyceride Turnover, Lipoprotein Lipase Activity, and Fat Cell Size in Adipose Tissue of Rats during the First 2 Weeks of Pregnancy

Triglyceride Turnover, Lipoprotein Lipase Activity, and Fat Cell Size in Adipose Tissue of Rats during the First 2 Weeks of Pregnancy

Respiratory Responses to Intravenous Infusion of Sodium Lactate in Male and Female Wistar Rats

Gender-dependent regulation of glutamate dehydrogenase expression in periportal and pericentral zones of rat liver lobules.

Contact Info

Product

Resources

About