Lipoprotein Lipase in Developing Rat Tissues: Differences between Wistar and Sprague-Dawley Rats

Galan, Xavier; Llobera, Miquel; Ramı́rez, Ignasi

doi:10.1159/000244002

Cited by 16 publications

(16 citation statements)

References 19 publications

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“…During the second half of the suckling period (wP 10-wP 21), LPL activity declines in the liver and increases in other tissues, such as the heart, skeletal muscle, and kidney, reaching adult levels at weaning (39)(40)(41). Meanwhile, the expression of STRA6 decreases in the lungs (27) and other extrahepatic tissues (27,42).…”

Section: Tablementioning

confidence: 99%

Vitamin A supplementation redirects the flow of retinyl esters from peripheral to central organs of neonatal rats raised under vitamin A–marginal conditions

Hodges

Tan

Green

et al. 2017

The American Journal of Clinical Nutrition

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Background: Vitamin A (VA; retinol) supplementation is used to reduce child mortality in countries with high rates of malnutrition. Existing research suggests that neonates (,1 mo old) may have a limited capacity to store VA in organs other than the liver; however, knowledge about VA distribution and kinetics in individual, nonhepatic organs is limited. Objective: We examined retinol uptake and turnover in nonhepatic organs, including skin, brain, and adipose tissue, in neonatal rats without and after VA supplementation. Design: Sprague-Dawley neonatal rats (n = 104) were nursed by mothers fed a VA-marginal diet (0.35 mg retinol/kg diet) and treated on postnatal day 4 with an oral dose of either VA (6 mg retinyl palmitate/g body weight) or canola oil (control), both containing 1.8 mCi of [ The RBP-ROH released from the liver was acquired mainly by the peripheral tissues but not retained efficiently, causing repeated recycling of retinol between plasma and tissues (541 compared with 5 times in the supplemented group and control group, respectively) and its rapid turnover in all organs, except the brain and white adipose tissue. Retinol stores in the liver lasted for w2 wk before being gradually transferred to other organs. Conclusions: VA supplementation administered in a single high dose during the first month after birth is readily acquired but not retained efficiently in peripheral tissues of neonatal rats, suggesting that a more frequent, lower-dose supplementation may be necessary to maintain steady VA concentrations in rapidly developing neonatal tissues.

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Section: Tablementioning

confidence: 99%

Vitamin A supplementation redirects the flow of retinyl esters from peripheral to central organs of neonatal rats raised under vitamin A–marginal conditions

Hodges

Tan

Green

et al. 2017

The American Journal of Clinical Nutrition

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“…More recent studies from our laboratory [12], in which lipoprotein lipase did not interfere with the activity measured, indicated that there were differences between rat strains in the developmental profile of hepatic lipase activity. In Wistar rats, hepatic lipase activity was lower in pups than in adult animals [12].…”

Section: Discussionmentioning

confidence: 76%

“…Because the activity measured in this period was sensitive to the high NaCl concentration in the assay system, the peak of enzyme activity has to be attributed to the cross-reaction produced by the well-documented peak of lipoprotein lipase in the liver during the perinatal period [23,25,27,[30][31][32][33]. More recent studies from our laboratory [12], in which lipoprotein lipase did not interfere with the activity measured, indicated that there were differences between rat strains in the developmental profile of hepatic lipase activity. In Wistar rats, hepatic lipase activity was lower in pups than in adult animals [12].…”

Section: Discussionmentioning

confidence: 93%

“…Hepatic lipase is sensitive to many physiological and pathological stimuli. Thus, development [12], gender [13], nutritional status [14,15], diet composition [16,17], and pathological situations such as diabetes [14,18], hypothyroidism [19], or hypercortisolism [18], all affect enzyme activity in the liver of several animal species. Administration of catecholamines produce a rapid decrease in liver hepatic lipase in rats [20,21].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Fasting on Hepatic Lipase Activity in the Liver of Developing Rats

Peinado-Onsurbe

Julve²,

Galan³

et al. 2000

Neonatology

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The effect of fasting on hepatic lipase was studied during postnatal development in the rat. It was found that fasting produced a significant decrease in hepatic lipase only in neonatal (1-day-old) and adult (60-day-old) rats. We studied the effect of fasting on the distribution of hepatic lipase between extracellular (heparin-releasable) and intracellular (liver-retained or residual) compartments in perfused livers, and on the secretion of hepatic lipase by isolated hepatocytes. Fasting had similar effects in neonates and adults: it decreased both the heparin-releasing and the residual activities in perfused livers, and also decreased the rate of hepatic lipase secretion by isolated hepatocytes. Finally, the effect of fasting on hepatic lipase mRNA relative abundance in developing rat livers was determined. No difference was observed among the groups studied. It is concluded that the mechanisms involved in the effect of fasting on hepatic lipase appear to be similar in neonates and adult animals and may involve the post-translational processing of the enzyme.

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“…One of these differences is the increase in maternal body weight with pregnancy, which is lower in Wistar than in Sprague-Dawley rats, the difference being mainly due to the lighter fetal body weight in the former. Rats from either group used in the present study had the same body weight at the onset of the experiment, but it is known that adult age-matched Wistar rats weigh less than Sprague-Dawley rats [24][25][26]. Thus, the Wistar rats used here were 2 weeks older than the Sprague-Dawley rats, a difference that could be responsible for the lower body weight gain of the former throughout the experiment.…”

Section: Discussionmentioning

confidence: 91%

A Sucrose-Rich Diet during Pregnancy Causes a Similar Response in Sprague-Dawley and Wistar Rats

López-Soldado

Munilla²,

Herrera³

2001

Ann Nutr Metab

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Background/Aims: In order to determine whether the response to a sucrose-rich diet (SRD) during pregnancy in the rat varies depending on the strain, the responsiveness to a SRD (63 g sucrose/100 g) during pregnancy in Wistar and Sprague-Dawley rats was studied. Methods: One group of rats of each strain was fed the SRD, whereas another group received the same diet except that sucrose was replaced by an equal amount of cornstarch. Half of the rats were mated, and all animals were studied 20 days later. Results: Initial body weight did not differ among groups, but final body weight of pregnant Wistar rats was lower than in Sprague-Dawley, and this difference corresponded to a decrease in fetal body weight in the former. Feeding a SRD did not modify pregnancy outcome in either rat strain. Plasma triglycerides increased with a SRD, although this effect was milder in Wistar pregnant rats than in the other groups. Adipose tissue lipoprotein lipase activity was lower in pregnant than in virgin rats, but no differences were found as result of either diet or rat strain. Liver triglyceride concentration increased in virgin rats fed SRD, the effect being greater in Sprague-Dawley than in Wistar rats. Conclusion: Differences in the response to a SRD in pregnant and virgin rats do not depend on the strain of rats used.

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Lipoprotein Lipase in Developing Rat Tissues: Differences between Wistar and Sprague-Dawley Rats

Cited by 16 publications

References 19 publications

Vitamin A supplementation redirects the flow of retinyl esters from peripheral to central organs of neonatal rats raised under vitamin A–marginal conditions

Vitamin A supplementation redirects the flow of retinyl esters from peripheral to central organs of neonatal rats raised under vitamin A–marginal conditions

Effect of Fasting on Hepatic Lipase Activity in the Liver of Developing Rats

A Sucrose-Rich Diet during Pregnancy Causes a Similar Response in Sprague-Dawley and Wistar Rats

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