Lipoprotein Profile in Canine Pancreatitis Induced with Oleic Acid.

Abstract

ABSTRACT. Lipid and lipoprotein concentrations and apolipoprotein profile were investigated in canine pancreatitis induced by infusion with oleic acid (OA) into the accessory pancreatic duct. Pancreatitis was diagnosed by physical, hematological, biochemical and pathological examinations. In OA-treated dogs, serum triglyceride (TG) concentration was increased; however, there were no changes in serum total cholesterol (TC) and phospholipid (PL) concentrations. Serum concentrations of TG, TC, PL and total lipids (TL) in beta lipoprotein and those of TC, PL and TL in pre-beta lipoprotein were increased and those of TC, PL and TL in alpha 1 lipoprotein were decreased. In apolipoprotein profile, the proportions of apolipoprotein B100 in low density lipoprotein fraction and apolipoprotein A-IV in high density lipoprotein fraction were increased. In addition, decreased proportion of apolipoprotein A-I and the appearance of serum amyloid A protein in high density lipoprotein fraction were observed. These results suggest that lipoprotein profiles observed in canine acute pancreatitis are attributed to the alterations in apolipoprotein compositions. -KEY WORDS: apolipoprotein, canine, lipid, lipoprotein, pancreatitis.J. Vet. Med. Sci. 60(4): 413-421, 1998 in clinical pancreatitis [15,27], increase of TG are not observed in these experimentally induced pancreatitis [4,27]. Therefore, hyperlipidemia in acute pancreatitis might not be reproduced by ligation of pancreatic duct and infusion with mixture of bile and trypsin. In this study, induction of canine acute pancreatitis was attempted by the infusion with oleic acid (OA) into pancreatic duct. Hyperlipidemia is brought by changes of lipoprotein metabolism. Apolipoproteins are structural proteins and participate in lipoprotein metabolism. Changes of apolipoprotein composition bring about abnormality in lipoprotein metabolism. Analysis of apolipoprotein is important to understand the changes of lipoprotein metabolism; however, there is no report on serum apolipoprotein profile in canine pancreatitis. The purpose of this study is to examine the effects of acute pancreatitis induced with OA on serum lipid and lipoprotein concentrations and apolipoprotein profile in dogs. MATERIALS AND METHODSExperimental animals: Eight male beagle dogs, weighing 10.5 to 14.5 kg and aged over one year were used. These dogs were divided into two groups. OA-treated dogs were injected with OA (Sigma Chemical Co., U.S.A.) and control dogs were injected with physiological saline (PS; Otsuka Seiyaku Co., Ltd., Tokyo).Surgical manipulation and inducing pancreatitis: Operative procedures were performed on the dogs after premedication with atropine sulfate (Tanabe Seiyaku Co., Ltd., Osaka) and xylazine hydrochloride (Bayer Japan Co., Ltd., Tokyo) and induction with sodium thiamylal (Yoshitomi Seiyaku Co., Ltd., Osaka) and under inhalation anesthesia with isoflurane (Dainabot Co., Ltd., Tokyo). Laparotomy through a midline approach and duodenotomy was done. A flexible 22-gauge needle was inserted via...

Canine Serum Amyloid A (SAA) Measured by Automated Latex Agglutination Turbidimetry Is Useful for Routine Sensitive and Specific Detection of Systemic Inflammation in a General Clinical Setting

“…SAA in dogs with and without systemic inflammation: The significant overlap performance of SAA demonstrated in the study confirms the results of previous studies of canine SAA [1,3,4,8], and extends the initial knowledge of the overlap performance of the LAT obtained as part of our validation of the assay [7]. Further, our comparison of the diagnostic performance of SAA with the performance of leukocytes, segmented and band neutrophils and body temperature suggests that routine measurements of SAA can be a useful adjunct to the information obtained by measuring these traditionally used inflammatory markers.…”

“…Serum amyloid A (SAA) is a major positive APP in dogs, and marked increases in concentrations are consequently observed during the APR [3]. Studies in several species have shown that SAA is a more sensitive marker of systemic inflammation than traditionally used parameters such as body temperature, leukocyte and neutrophil counts [12,19], and SAA can be used as a diagnostic marker of systemic inflammation in several species, including dogs [1,4,8,21]. The commercial availability of diagnostic assays has, however, been limited to resource and time consuming methods, and so far measurements of canine SAA have not been implemented in routine veterinary clinical biochemistry [3].…”

Estudio del perfil lipídico canino por edad y sexo

“…Existen muchas variables que pueden contribuir a la alteración del perfil lipídico de los caninos y, en consecuencia, a un aumento de este; entre estas variables se encuentra la obesidad, que se puede dar por numerosos factores, tanto ambientales como sociales, ya que la falta de ejercicio de los animales, la sobrealimentación o una dieta desequilibrada con una alimentación ad libitum de dietas ricas en grasa, la raza, la genética, la esterilización, medicamentos como los corticoesteroides y los progestágenos están relacionados como causantes de la obesidad (22). Por eso numerosos análisis identificaron que los perros obesos presentan un col-total, triglicéridos, col-VLDL, col-LDL, más alto que los caninos normales, mientras que el col-HDL es menor en los obesos presentándo-se una situación poco deseable por el riesgo de enfermedades cardiovasculares (5,9,22,23). Por otra parte, se encontró que una modificación en la composición de la dieta, y un control en el consumo de energía mejoran las concentraciones de lípi-dos en estos animales produciendo así un beneficio (22,23), ya que en el estudio los caninos alimentados con regímenes hipergrasos presentan valores altos de colesterol total, col-LDL y niveles bajos de col-HDL (9,(19)(20)(21).…”

“…Por eso numerosos análisis identificaron que los perros obesos presentan un col-total, triglicéridos, col-VLDL, col-LDL, más alto que los caninos normales, mientras que el col-HDL es menor en los obesos presentándo-se una situación poco deseable por el riesgo de enfermedades cardiovasculares (5,9,22,23). Por otra parte, se encontró que una modificación en la composición de la dieta, y un control en el consumo de energía mejoran las concentraciones de lípi-dos en estos animales produciendo así un beneficio (22,23), ya que en el estudio los caninos alimentados con regímenes hipergrasos presentan valores altos de colesterol total, col-LDL y niveles bajos de col-HDL (9,(19)(20)(21). Otro factor que se encontró para contrarrestar estos riesgos antes mencionados es el aumento del ejercicio físico de los animales más sedentarios, ya que estudios revelan que los caninos menos activos presentan niveles significativamente altos de col-VLDL y col-LDL en comparación con animales que tienen una rutina de ejercicio o de trabajo (21).…”

Investigation of Hypertriglyceridemia in Healthy Miniature Schnauzers

“…2 Secondary hypertriglyceridemia, which is the most common form in dogs, usually is the result of an endocrine disorder such as hypothyroidism, diabetes mellitus, or hyperadrenocorticism. 4,5 Other possible causes of secondary hypertriglyceridemia include pancreatitis, 6 obesity, 7,8 lymphoma, 9 or administration of certain drugs (eg, glucocorticoids). 10 A presumptive diagnosis of idiopathic hypertriglyceridemia can only be made when other causes of secondary hypertriglyceridemia have been ruled out.…”