Liposomal co-delivery of daptomycin and clarithromycin at an optimized ratio for treatment of methicillin-resistantStaphylococcus aureusinfection

Abstract

(2015) Liposomal co-delivery of daptomycin and clarithromycin at an optimized ratio for treatment of methicillin-resistant Staphylococcusaureus infection, Drug Delivery, 22:5, 627-637, DOI: 10.3109/10717544.2014 CD]) at an optimized mass ratio of 1:32 were generated and characterized using dynamic light scattering and electron microscopy. In vitro and in vivo approaches were used to compare liposome effects on MRSA. Results: PL[CD] were stable, with a mean (±SD) vesicle diameter of 98.2 ± 2.21 nm and encapsulation efficiency of 94.71 ± 1.37% (daptomycin) and 92.94 ± 1.21% (clarithromycin). Compared with daptomycin-only liposomes, PL [CD] showed significantly enhanced anti-MRSA activity in vitro and significantly reduced MRSA bacterial load and increased host survival in vivo. Discussion: Co-delivery of daptomycin with clarithromycin produced significant anti-MRSA activity in the presence of only one-thirtieth of the concentration required in liposomes containing daptomycin only. Conclusion: These findings suggested that concurrent liposomal delivery of daptomycin and clarithromycin has the potential to be an effective and less toxic treatment for MRSA infections.

The effective combination therapy against human osteosarcoma: doxorubicin plus curcumin co-encapsulated lipid-coated polymeric nanoparticulate drug delivery system

“…The DEE of both two drug loadings in all kinds of LPNs showed no significant variations (p40.05). These results suggest that the loading of two drugs did not affect the encapsulation efficiency of each other (Li Y et al, 2015). This phenomenon may be due to the stable ability of the LPNs.…”

“…The IC 50 values of drug loaded LPNs were many folds dose advantage over the free drug solutions. These results could be explained as follows: Firstly, the construction of LPN systems could improve the delivery of drugs thus gain better efficiency than free drugs; Secondly, the combined antitumor effect of the dual drugs loaded LPNs performed better than the single drug loaded ones (Li Y et al, 2015).…”

Compartmentalized Encapsulation of Two Antibiotics in Porous Nanoparticles: an Efficient Strategy to Treat Intracellular Infections

“…Moreover, polymeric NPs or liposomes are monocompartmented and, as a general trend, when drugs were coencapsulated, they impeded each other leading to dramatically reduced loadings. For example, the loading of daptomycin in liposomes was drastically reduced from 4.8 to 0.29 wt% when clarithromycin was coencapsulated …”

Nanoparticle approaches against bacterial infections

“…In contrast, the corresponding liposomal formulation encapsulating both gentamicin and ceftazidime showed a synergistic effect that led to a shorter course of treatment at lower cumulative doses 83 . The benefit of ratiometric delivery using liposomes was also reported in other combination therapies in treating S. aureus 84, 85 , M. tuberculosis 73, 74 , Mycobacterium avium (M. avium) 86, 87 , and H. pylori 88, 89 .…”