Liposomal drug delivery of Corchorus olitorius leaf extract containing phytol using design of experiment (DoE): In-vitro anticancer and in-vivo anti-inflammatory studies

Shariare, Mohammad Hossain; Noor, Humaira; Khan, Junayet Hossain; Uddin, Jamal; Ahamad, Syed Rizwan; Altamimi, Mohammad A.; Alanazi, Fars K.; Kazi, Mohsin

doi:10.1016/j.colsurfb.2020.111543

Cited by 22 publications

(22 citation statements)

References 20 publications

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“…All four plants described in this chapter was extracted using maceration method (Figure 3). Plant extraction, its phytoconstituents identification and quantification were performed using UHPLC and GC-MS analytical methods [80][81][82][83]. Results from UHPLC data showed that the concentration of thymoquinone was 2.28 ± 0.68 mg/g of black seed oil [80].…”

Section: Plant Extraction Identification and Quantification By Uhplc And Gc-msmentioning

confidence: 99%

“…2-hexadecan-1-ol (phytol) etc [82]. Chromatographic results also suggest that 500 μg EGCG was present in one milliliter of green tea leaf, which was extracted using water as solvent at different temperatures [83].…”

Section: Plant Extraction Identification and Quantification By Uhplc And Gc-msmentioning

confidence: 99%

“…This phenomenon also probably related to low average size of PBNDDS developed at these conditions, while PBNDDS with high average particle size having high entrapment or loading efficiency. Polydispersity of PBNDDS was markedly affected by processing temperature and stirring speed and suggesting that batches processed at low temperature and high stirring speed found to be lessly polydispersed [81,82].…”

Section: Figure 7 Two Way Interaction Plots Of [A] Process Parameters and [B] Formulation Attributes For Average Particle Size Of Pbnddsmentioning

confidence: 99%

“…Formulation attributes -ratio of drug: phospholipid and phospholipid: cholesterol, solvent system and its properties (phytoconstituents solubility, pH of the solvent), drug concentration found to have major impact on average particle size, polydispersity and entrapment efficiency of PBNDDS of phytoconstituents [80][81][82][83].…”

Section: Figure 7 Two Way Interaction Plots Of [A] Process Parameters and [B] Formulation Attributes For Average Particle Size Of Pbnddsmentioning

confidence: 99%

“…It is very imperative to find out the optimum level and amount of each of these process parameters and formulation attributes to achieve low average size with high entrapment or loading of phytoconstituents for PBNDDS. It was evident that not only the impact of individual parameters but its interactions also exhibited marked impact on the average size and loading of phytoconstituents for PBNDDS (Figure 7) [81][82][83].…”

Section: Figure 7 Two Way Interaction Plots Of [A] Process Parameters and [B] Formulation Attributes For Average Particle Size Of Pbnddsmentioning

confidence: 99%

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Phospholipid Based Nano Drug Delivery Systems of Phytoconstituents

Shariare¹,

Kazi²

2022

Smart Drug Delivery

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The development of phytochemistry and phyto-pharmacology has enabled elucidation of composition and biological activities of several medicinal plant constituents. However phytoconstituents are poorly absorbed due to their low aqueous solubility, large molecular size and poor membrane permeability when taken orally. Nanotechnology based drug delivery systems can be used to improve the dissolution rate, permeability and stability of these phytoconstituents. The current chapter aims to present the extraction of phytoconstituents, their identifications, and development/utilization of phospholipid based nano drug delivery systems (PBNDDS). The content of the chapter also provides characteristic features, in-vitro, in-vivo evaluations and stability performance of PBNDDS. The results from the UHPLC and GC-MS showed different phytoconstituents in the extracted samples with quantitative value. Dynamic light scattering (DLS) data showed PBNDDS of different phytoconstituents in the range of 50–250 nm with PDI value of 0.02–0.5, which was also confirmed by the electron microscopic data. Phytoconstituents loading or entrapment for PBNDDS was in the range of 60–95%. PBNDDS exhibited better in-vitro and in-vivo performance with improved Physico-chemical stability.

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Section: Plant Extraction Identification and Quantification By Uhplc And Gc-msmentioning

confidence: 99%

Section: Plant Extraction Identification and Quantification By Uhplc And Gc-msmentioning

confidence: 99%

Section: Figure 7 Two Way Interaction Plots Of [A] Process Parameters and [B] Formulation Attributes For Average Particle Size Of Pbnddsmentioning

confidence: 99%

Section: Figure 7 Two Way Interaction Plots Of [A] Process Parameters and [B] Formulation Attributes For Average Particle Size Of Pbnddsmentioning

confidence: 99%

Section: Figure 7 Two Way Interaction Plots Of [A] Process Parameters and [B] Formulation Attributes For Average Particle Size Of Pbnddsmentioning

confidence: 99%

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Phospholipid Based Nano Drug Delivery Systems of Phytoconstituents

Shariare¹,

Kazi²

2022

Smart Drug Delivery

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Zein/fucoidan‐coated phytol nanoliposome: preparation, characterization, physicochemical stability, in vitro release, and antioxidant activity

Chen,

Wang,

et al. 2024

J Sci Food Agric

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BACKGROUNDTo improve phytol bioavailability, a novel method of magnetic stirring and high‐pressure homogenization (HPH) combination was used to prepare zein/fucoidan‐coated phytol nanoliposomes (P‐NL‐ZF). The characterization, the simulated in vitro digestion, and the antioxidant activity of these phytol nanoliposomes from the different processes have been studied.RESULTSBased on the results of dynamic light scattering (DLS) and gas chromatography–mass spectrometer (GC–MS) analysis, P‐NL‐ZF prepared through the combination of magnetic stirring and HPH exhibited superior encapsulation efficiency at 76.19% and demonstrated exceptional physicochemical stability under a series of conditions, including storage, pH, and ionic in comparison to single method. It was further confirmed that P‐NL‐ZF by magnetic stirring and HPH displayed a uniform distribution and regular shape through transmission electron microscopy (TEM). Fourier‐transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC) analysis showed that electrostatic interactions and hydrogen bonding were the primary driving forces for the formation of composite nanoliposomes. Additionally, an in vitro digestion study revealed that multilayer composite nanoliposomes displayed significant and favorable slow‐release properties (58.21%) under gastrointestinal conditions compared with traditional nanoliposomes (82.36%) and free phytol (89.73%). The assessments of chemical and cell‐based antioxidant activities demonstrated that the coating of zein/fucoidan on phytol nanoliposomes resulted in enhanced effectiveness in scavenging activity of ABTS free radical and hydroxyl radical and mitigating oxidative damage to HepG2 cells.CONCLUSIONBased on our studies, the promising delivery carrier of zein/fucoidan‐coated nanoliposomes is contributed to the encapsulation of hydrophobic natural products and enhancement of their biological activity. © 2024 Society of Chemical Industry.

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In‐Vitro Antimicrobial, Antidiabetic and Anticancer Activities of Calyptocarpus Vialis Extract and its Integration with Computational Studies

Bachhar,

Joshi,

Shekher Mishra

et al. 2024

ChemistrySelect

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Calyptocarpus vialis (C. vialis), a plant of Asteraceae family exhibits nutritional value, antioxidant properties and has potential for various other biomedical applications. The present study is focused to assess the antimicrobial, antidiabetic, and anticancer potential of C. vialis extract and its integration with computational studies. The antimicrobial activity is performed against the Staphylococcus aureus (S. aureus), Bacillus subtilis (B. subtilis) pseudomonas aeruginosa (P. aeruginosa), Escherichia coli (E. coli) bacteria and Saccharomyces cerevisiae (S. cerevisiae), Candida albicans (C. albicans) fungi using disc diffusion method. Maximum Zone of Inhibition (ZOI) of 37±3.2 and 35±2.9 mm is obtained for S. aureus and B. subtilis respectively, whereas ZOI of 39±2.8 mm is obtained against C. albicans at dose of 500 μg/mL. The extract shows strong α‐amylase inhibition activity with IC50 ~46.8±0.81 μg/mL inferring about its antidiabetic potential. Anticancer activity of C. vialis extract is checked against HCT‐116 and DU‐145 cell lines using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium‐bromide (MTT) assay, which shows excellent anticancer activity with IC50 value of 38.09±0.65 μg/ml and 36.34±1.05 μg/ml respectively. The obtained results are further verified using computational studies considering interaction among phytochemicals and selected targets. Extract is found potent for biological activities and promising candidate for development of new drugs.

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Liposomal drug delivery of Corchorus olitorius leaf extract containing phytol using design of experiment (DoE): In-vitro anticancer and in-vivo anti-inflammatory studies

Cited by 22 publications

References 20 publications

Phospholipid Based Nano Drug Delivery Systems of Phytoconstituents

Phospholipid Based Nano Drug Delivery Systems of Phytoconstituents

Zein/fucoidan‐coated phytol nanoliposome: preparation, characterization, physicochemical stability, in vitro release, and antioxidant activity

In‐Vitro Antimicrobial, Antidiabetic and Anticancer Activities of Calyptocarpus Vialis Extract and its Integration with Computational Studies

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