Liposomal Drug Deposits in Poly(Dopamine) Coatings: Effect of Their Composition, Cell Type, Uptake Pathway Considerations, and Shear Stress

Abstract

Implantable devices equipped with coatings which have the ability to carry and deliver active compounds are of great interest. We report the assembly of liposome-containing poly(dopamine) films, and their interaction with adhering cells. The liposome composition is varied by adding lipophilic dopamine-conjugates and charged lipids. The cell mean fluorescence (CMF) of adhering cells due to the internalization of fluorescent cargo is found to be similar for coatings with the lipophilic-dopamine conjugates, while the charge affects the amount and location of the internalized cargo. The uptake mechanism for cargo by myoblasts using chemical inhibitors is found to be dependent on the used type of liposome. The CMF is significantly reduced for endothelial cells adhering to coatings with applied shear stress.

Mixed poly(dopamine)/poly(l-lysine) (composite) coatings: from assembly to interaction with endothelial cells

“…S7a †). 20 The CMF and uptake efficiency were found to be significantly higher using M n as a substrate compated to L n /PDA after 6 h adhesion time. The cell mean fluorescence (CMF) (Fig.…”

“…The effect of the polymer precursor layer on the subsequent adhesion of the composite coating was assessed. 20 This fact indicated that PLL s was interfering with the composite film deposition. Δf (Fig.…”

“…Mixtures of DA and PLL s with either zwitterionic liposomes (L zw ), negatively charged liposomes (L n ) or positively charged liposomes (L p ) were labeled with M zw,PLLs , M n,PLLs and M p,PLLs , respectively. 20 A capping layer of PDA/PLL s was deposited onto all the liposome pre-coated surfaces and compared to PDA capping layers. 6a, stripy bars) and ΔD (ESI Fig.…”

“…Coatings assembled via the sequential deposition of interacting polymers (the layer-by-layer (LbL) technique) were found to be particularly promising for surface-mediated drug delivery (SMDD) of diverse cargo due to its flexibility, versatility and diversity. 17 Further, liposomes have been incorporated into PDA-based films 11,[18][19][20] or coated with a PDA-based polymer layer 21 as drug deposits towards their use in SMDD. 5,6 PDA films have so far been proven to be biocompatible, and they can be deposited on virtually any surface independent of shape and material or post-modified via thiols and amines, but their structure remains to be elucidated unambiguously.…”

Tannic acid and cholesterol–dopamine as building blocks in composite coatings for substrate‐mediated drug delivery

“…Liposomes have been incorporated into LbL films via charge interactions between the liposomes and the polymer layers or by using a cholesterol‐modified polymer for non‐covalent anchoring of the liposomes to the polymer film . Alternatively, polydopamine (PDA) coatings, deposited in one step via the oxidative self‐polymerization of dopamine (DA) at slightly basic pH, have proven successful for assembling composite coatings using liposomal subunits . In contrast to the LbL‐based assembly method, one‐step procedures can be less labour‐intensive.…”