Liposome clearance in mice: the effect of a separate and combined presence of surface charge and polymer coating

Levchenko, Tatiana S; Rammohan, Ram; Lukyanov, Anatoly N.; Whiteman, Kathleen R.; Torchilin, Vladimir P.

doi:10.1016/s0378-5173(02)00129-1

Cited by 295 publications

(205 citation statements)

References 23 publications

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“…However, at a longer acquisition time (30 min), the distribution clearly showed liver accumulation. These differential distributions are in agreement with published works on other types of NPs (21)(22)(23).…”

Section: Resultssupporting

confidence: 92%

Nanoprobes with near-infrared persistent luminescence for in vivo imaging

Chermont

Chanéac

Séguin

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

763

501

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Fluorescence is increasingly used for in vivo imaging and has provided remarkable results. Yet this technique presents several limitations, especially due to tissue autofluorescence under external illumination and weak tissue penetration of low wavelength excitation light. We have developed an alternative optical imaging technique by using persistent luminescent nanoparticles suitable for small animal imaging. These nanoparticles can be excited before injection, and their in vivo distribution can be followed in real-time for more than 1 h without the need for any external illumination source. Chemical modification of the nanoparticles' surface led to lung or liver targeting or to long-lasting blood circulation. Tumor mass could also be identified on a mouse model. biodistribution ͉ in vivo optical imaging ͉ nanoparticles ͉ phosphorescent nanoparticles

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Section: Resultssupporting

confidence: 92%

Nanoprobes with near-infrared persistent luminescence for in vivo imaging

Chermont

Chanéac

Séguin

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

763

501

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“…administration, believed to result from rapid uptake by the reticuloendothelial system (RES) (25). Grafting liposomes with polyethylene glycol (PEG) has been shown to prolong liposome circulation in vivo (26)(27)(28). The presence of PEG attracts water to the liposome surface and provides a barrier against opsonins and cells of the RES (29).…”

Section: Introductionmentioning

confidence: 99%

“…The presence of PEG attracts water to the liposome surface and provides a barrier against opsonins and cells of the RES (29). In addition, PEG neutralizes the surface charge of liposomes, decreasing the interaction between charged phospholipid head groups and opsonizing proteins (26). PEGylation of FVIII-PS liposomes resulted in a further reduction of immune response compared to the un-PEGylated formulation but provided only a modest improvement in the pharmacokinetic profile (30).…”

Section: Introductionmentioning

confidence: 99%

PEGylation of a Factor VIII–Phosphatidylinositol Complex: Pharmacokinetics and Immunogenicity in Hemophilia A Mice

Peng

Kosloski

Nakamura

et al. 2011

AAPS J

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Abstract. Hemophilia A is an X-linked bleeding disorder caused by the deficiency of factor VIII (FVIII). Exogenous FVIII is administered therapeutically, and due to a short half-life, frequent infusions are often required. Fifteen to thirty-five percent of severe hemophilia A patients develop inhibitory antibodies toward FVIII that complicate clinical management of the disease. Previously, we used phosphatidylinositol (PI) containing lipidic nanoparticles to improve the therapeutic efficacy of recombinant FVIII by reducing immunogenicity and prolonging the circulating half-life. The objective of this study is to investigate further improvements in the FVIII-PI formulation resulting from the addition of polyethylene glycol (PEG) to the particle. PEGylation was achieved by passive transfer of PEG conjugated lipid into the FVIII-PI complex. PEGylated FVIII-PI (FVIII-PI/PEG) was generated with high association efficiency. Reduced activity in vitro and improved retention of activity in the presence of antibodies suggested strong shielding of FVIII by the particle; thus, in vivo studies were conducted in hemophilia A mice. Following intravenous administration, the apparent terminal half-life was improved versus both free FVIII and FVIII-PI, but exposure determined by area under the curve was reduced. The formation of inhibitory antibodies after subcutaneous immunization with FVIII-PI/PEG was lower than free FVIII but resulted in a significant increase in inhibitors following intravenous administration. Passive transfer of PEG onto the FVIII-PI complex does not provide any therapeutic benefit.

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“…A single intravenous dose of indinavir-containing nanoparticles in mice with HIV+ brain macrophages led to drug levels in the brain for up to 14 days and reduced virus replication in the brain (Dou et al, 2009). Nevertheless, optimization of nanocomplexes by coating their surfaces with polyethylene glycol resulted in sustained levels in the blood of mice and less clearing by macrophages (Levchenko et al, 2002).…”

Section: New Delivery Methodsmentioning

confidence: 99%

Use of Animal Models for Anti-HIV Drug Development

Ambrose¹

2012

Antiviral Drugs - Aspects of Clinical Use and Recent Advances

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Liposome clearance in mice: the effect of a separate and combined presence of surface charge and polymer coating

Cited by 295 publications

References 23 publications

Nanoprobes with near-infrared persistent luminescence for in vivo imaging

Nanoprobes with near-infrared persistent luminescence for in vivo imaging

PEGylation of a Factor VIII–Phosphatidylinositol Complex: Pharmacokinetics and Immunogenicity in Hemophilia A Mice

Use of Animal Models for Anti-HIV Drug Development

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