Liposome model experiment for the study of assumed membrane damage in porphyria cutanea tarda

Kószó, F.; Siklósi, Cs.; Simon, N.

doi:10.1016/0005-2736(74)90057-1

Cited by 7 publications

(1 citation statement)

References 18 publications

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“…These results suggest that although an endogenous ICE - CBF pathway is present in Arabidopsis system and that enables the ectopic VaICE1,2 transgene expression to influence the plants' freezing-tolerance capacity. There are also considerable evidences to suggest that proline [63]–[65], MDA [14], [66], [67], and electrolyte leakage [25], [68], contribute to an enhancement of freezing tolerance. Our results confirmed that the greater tolerance of VaICE1 or VaICE2 -overexpressing plants to cold stress positively linked to elevated proline levels, and negatively correlated to the reduction of MDA content as well as electrolyte leakage.…”

Section: Discussionmentioning

confidence: 99%

Chinese Wild-Growing Vitis amurensis ICE1 and ICE2 Encode MYC-Type bHLH Transcription Activators that Regulate Cold Tolerance in Arabidopsis

Jiao

et al. 2014

PLoS ONE

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Winter hardiness is an important trait for grapevine breeders and producers, so identification of the regulatory mechanisms involved in cold acclimation is of great potential value. The work presented here involves the identification of two grapevine ICE gene homologs, VaICE1 and VaICE2, from an extremely cold-tolerant accession of Chinese wild-growing Vitis amurnensis, which are phylogenetically related to other plant ICE1 genes. These two structurally different ICE proteins contain previously reported ICE-specific amino acid motifs, the bHLH-ZIP domain and the S-rich motif. Expression analysis revealed that VaICE1 is constitutively expressed but affected by cold stress, unlike VaICE2 that shows not such changed expression as a consequence of cold treatment. Both genes serve as transcription factors, potentiating the transactivation activities in yeasts and the corresponding proteins localized to the nucleus following transient expression in onion epidermal cells. Overexpression of either VaICE1 or VaICE2 in Arabidopsis increase freezing tolerance in nonacclimated plants. Moreover, we show that they result in multiple biochemical changes that were associated with cold acclimation: VaICE1/2-overexpressing plants had evaluated levels of proline, reduced contents of malondialdehyde (MDA) and decreased levels of electrolyte leakage. The expression of downstream cold responsive genes of CBF1, COR15A, and COR47 were significantly induced in Arabidopsis transgenically overexpressing VaICE1 or VaICE2 upon cold stress. VaICE2, but not VaICE1 overexpression induced KIN1 expression under cold-acclimation conditions. Our results suggest that VaICE1 and VaICE2 act as key regulators at an early step in the transcriptional cascade controlling freezing tolerance, and modulate the expression levels of various low-temperature associated genes involved in the C-repeat binding factor (CBF) pathway.

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