Liposome ocular delivery systems

Meisner, Dale; Mezei, Michael

doi:10.1016/0169-409x(95)00016-z

Cited by 132 publications

(61 citation statements)

References 52 publications

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“…The common problems associated with liposomes are that they are in liquid form, which limits their pharmaceutical formulation feasibility. Moreover, most methods of sterilization are considered unsuitable for liposomes (Meisner & Mezei, 1995). The heating involved in autoclaving can irreversibly damage their vesicular structure and filtration reduces the vehicle to an average of 200 nm which limits their application (Meisner & Mezei, 1995).…”

Section: • Metabolism By Enzymesmentioning

confidence: 99%

“…Liposomes are membrane-like lipid bilayer vesicles surrounding aqueous compartments (Meisner & Mezei, 1995). The common problems associated with liposomes are that they are in liquid form, which limits their pharmaceutical formulation feasibility.…”

Section: • Metabolism By Enzymesmentioning

confidence: 99%

“…Moreover, most methods of sterilization are considered unsuitable for liposomes (Meisner & Mezei, 1995). The heating involved in autoclaving can irreversibly damage their vesicular structure and filtration reduces the vehicle to an average of 200 nm which limits their application (Meisner & Mezei, 1995). Hironaka et al (2009) designed a sub-micron sized liposomal drug delivery system capable of delivering hydrophilic drugs to the posterior segment of the eye (Hironaka et al, 2009).…”

Section: • Metabolism By Enzymesmentioning

confidence: 99%

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Solid lipid nanoparticles for ocular drug delivery

2010

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Section: • Metabolism By Enzymesmentioning

confidence: 99%

Section: • Metabolism By Enzymesmentioning

confidence: 99%

Section: • Metabolism By Enzymesmentioning

confidence: 99%

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Solid lipid nanoparticles for ocular drug delivery

2010

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“…Due to their structure, chemical composition and colloidal size, which all can be well controlled by preparation methods, liposomes show numerous properties that may be useful in various applications (Cho et al, 2009;Meisner & Mezei, 1995;Yang et al, 2011). These properties point to several possible applications of liposomes such as their usage as solubilizers, dispersants, sustained release systems, stabilizers, protective agents, and microencapsulation systems.…”

Section: Introductionmentioning

confidence: 99%

Fusogenic pH sensitive liposomal formulation for rapamycin: Improvement of antiproliferative effect

Ghanbarzadeh

Khorrami

Khosroshahi

et al. 2014

Pharmaceutical Biology

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Context: Liposomes are increasingly employed to deliver chemotherapeutic agents, antisense oligonucleotides, and genes to various therapeutic targets. Objective: The present investigation evaluates the ability of fusogenic pH-sensitive liposomes of rapamycin in increasing its antiproliferative effect on human breast adenocarcinoma (MCF-7) cell line. Materials and methods: Cholesterol (Chol) and dipalmitoylphosphatidylcholine (DPPC) (DPPC:Chol, 7:3) were used to prepare conventional rapamycin liposomes by a modified ethanol injection method. Dioleoylphosphatidylethanolamine (DOPE) was used to produce fusogenic and pH-sensitive properties in liposomes simultaneously (DPPC:Chol:DOPE, 7:3:4.2). The prepared liposomes were characterized by their size, zeta potential, encapsulation efficiency percent (EE%), and chemical stability during 6 months. The antiproliferative effects of both types of rapamycin liposomes (10, 25, and 50 nmol/L) with optimized formulations were assessed on MCF-7 cells, as cancerous cells, and human umbilical vein endothelial cells (HUVEC), as healthy cells, employing the diphenyltetrazolium bromide (MTT) assay for 72 h. Results and discussion: The particle size, zeta potential, and EE% of the liposomes were 165 AE 12.3 and 178 AE 15.4 nm, À39.6 AE 1.3, and À41.2 AE 2.1 mV as well as 76.9 AE 2.6 and 76.9 AE 2.6% in conventional and fusogenic pH-sensitive liposomes, respectively. Physicochemical stability results indicated that both liposome types were relatively stable at 4 C than 25 C. In vitro antiproliferative evaluation showed that fusogenic pH-sensitive liposomes had better antiproliferative effects on MCF-7 cells compared to the conventional liposomes. Conversely, fusogenic pH-sensitive liposomes had less cytotoxicity on HUVEC cell line. KeywordsAntiproliferative effect, breast cancer, fusogenic pH-sensitive liposome, rapamycin History

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“…The application of liposomes in ophthalmic drug delivery is immense; 20,32 however, none of the liposomal drugs have been approved for ocular administration. 33,34 Despite liposomes' demonstrated improvements in precorneal retention, sustained drug release, and transcorneal permeation, they still face challenges in their limited long-term physical stability and drug-loading capacity due to the inherent complex nature of their structures.…”

mentioning

confidence: 99%

Supercritical fluid-mediated liposomes containing cyclosporin A for the treatment of dry eye syndrome in a rabbit model: comparative study with the conventional cyclosporin A emulsion

Karn¹,

Kim²,

Kang³

et al. 2014

IJN

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Background The objective of this study was to compare the efficacy of cyclosporin (CsA)-encapsulated liposomes with the commercially available CsA emulsion (Restasis ® ) for the treatment of dry eye syndrome in rabbits. Methods Liposomes containing CsA were prepared by the supercritical fluid (SCF) method consisted of phosphatidylcholine from soybean (SCF-S100) and egg lecithins (SCF-EPCS). An in vitro permeation study was carried out using artificial cellulose membrane in Franz diffusion cells. Dry eye syndrome was induced in male albino rabbits and further subdivided into untreated, Restasis ® -treated, EPCS, and S100-treated groups. Tear formation in the dry-eye-induced rabbits was evaluated using the Schirmer tear test. All formulations were also evaluated by ocular irritation tests using the Draize eye and winking methods with the determination of CsA concentration in rabbit tears. Results After the treatment, the Schirmer tear test value significantly improved in EPCS-treated ( P =0.005) and S100-treated ( P =0.018) groups compared to the Restasis ® -treated group. The AUC 0–24 h for rabbit’s tear film after the administration of SCF-S100 was 32.75±9.21 μg·h/mg which was significantly higher than that of 24.59±8.69 μg·h/mg reported with Restasis ® . Liposomal CsA formulations used in this study showed lower irritation in rabbit eyes compared with Restasis ® . Conclusion These results demonstrate that the novel SCF-mediated liposomal CsA promises a significant improvement in overcoming the challenges associated with the treatment of dry eyes.

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Liposome ocular delivery systems

Cited by 132 publications

References 52 publications

Solid lipid nanoparticles for ocular drug delivery

Solid lipid nanoparticles for ocular drug delivery

Fusogenic pH sensitive liposomal formulation for rapamycin: Improvement of antiproliferative effect

Supercritical fluid-mediated liposomes containing cyclosporin A for the treatment of dry eye syndrome in a rabbit model: comparative study with the conventional cyclosporin A emulsion

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