Liposomes as Gene Carriers: Efficient Transformation of Mouse L Cells by Thymidine Kinase Gene

Schaefer-Ridder, Maria; Wang, Yuan; Hofschneider, Peter Hans

doi:10.1126/science.7053567

Cited by 153 publications

(41 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…12 Cavifor the uptake of plasmid DNA into mammalian cells tation is well known as a powerful mechanism of tissue have been developed in the past, including coprecipidamage induction based on severe ultrastructural altertation with calcium phosphate, 1 use of polycations or lipations at the cellular and subcellular level. 13 Whereas a ids to form complexes with DNA, 2 encapsidation of plasdefined portion of shock wave-exposed cells is commid DNA into liposomes or erythrocyte ghosts, [3][4][5] pletely destroyed and reduced to cell debris (dependent exposure of cells to rapid pulses of high-voltage current, upon the applied number of discharges and their pulse ie electroporation, 6,7 receptor-mediated endocytosis of energy), the majority of cells surviving the shock wave-DNA-ligand complexes 8 and introduction of DNA into exposure procedure has been shown to continue to procells by direct microinjection 9 or on high-velocity tungliferate at a near normal rate. 14 Interestingly, different cell sten microprojectiles.…”

mentioning

confidence: 99%

Shock wave permeabilization as a new gene transfer method

Lauer

Bürgelt

Squire³

et al. 1997

Gene Ther

136

View full text Add to dashboard Cite

Uptake of naked functional DNA into mammalian cells can eukaryotic cells display a temporary increase in membrane be achieved by a number of physical methods. However, permeability. This effect was shown to be caused by cavifor most of these techniques possibilities for therapeutic in tation resulting in the transient generation of cell pores vivo applications -especially to solid organs -are often which allows the direct transfer of naked plasmid DNA. limited. In this report, we describe shock wave permeabilizShockwave transfection of a variety of cell lines was demation as a new physical gene transfer method, which can onstrated. Since shock waves can be well focused within be easily applied, provides great flexibility in the size and particular body regions, future applications of extracorporsequence of the DNA molecules to be delivered, and which ally generated shock waves to tissues simultaneously pershould exhibit an advantageous security profile in vivo.fused with DNA solutions might open up the possibility of Upon exposure to lithotripter-generated shock waves achieving a regionally enhanced in vivo gene transfer.

show abstract

mentioning

confidence: 99%

Shock wave permeabilization as a new gene transfer method

Lauer

Bürgelt

Squire³

et al. 1997

Gene Ther

136

View full text Add to dashboard Cite

show abstract

“…Nucleic acids ( 14,15), proteins (16), and antitumor drugs (17) can become cellassociated and biologically active, both in vitro and in vivo, using liposomes as transmembrane vectors. The intracellular content of these macromolecules can be increased severalfold.…”

mentioning

confidence: 99%

Protection against oxygen toxicity by intravenous injection of liposome-entrapped catalase and superoxide dismutase.

Turrens¹,

Crapo²,

Freeman³

1984

J. Clin. Invest.

456

141

View full text Add to dashboard Cite

Absra act. Survival of rats exposed to 100% oxygen was increased from 69.5±1.5 to 118.1±9.9 h (mean±SEM, P < 0.05) when liposomes containing catalase and superoxide dismutase were injected intravenously before and during exposure. The increased survival time in 100% oxygen was also associated with significantly less fluid in the pleural cavity. Rats injected with catalaseand superoxide dismutase-containing liposomes, which had increased survival in 100% oxygen, had increased lung wet weight upon autopsy compared with saline-injected controls (2.9±0.2 g/lung vs. 4.8±0.4 g/lung, mean±SE, P < 0.05). Intravenous injection of control liposomes along with catalase and superoxide dismutase in the suspending buffer decreased the mean pleural effusion volume 89% and had no significant effect on survival time. Lung catalase and superoxide dismutase activities were increased 3.1-and 1.7-fold, respectively, 2 h after a single intravenous injection of liposomes containing catalase or superoxide dismutase. Superoxide dismutase activity was also significantly greater than controls in both air-and 100% oxygen-exposed rat lungs, when enzyme activity was assayed 24 h after cessation of injection of control and oxygen-exposed rats with enzymecontaining liposomes every 12 h for 36 h. Free superoxide dismutase and catalase injected intravenously in the absence of liposomes did not increase corresponding lung enzyme activities, affect pleural effusion volume, lung wet weight, or extend the mean survival time of rats Preliminary results of these studies were presented at the Third International Conference on superoxide and superoxide dismutases, Ellenville, NY, October 1982.

show abstract

“…A nurober of methods for DNA transfection of a variety of mammalian cell lines have been previously described including the use of DEAE dextran (McCutchan and Pagano, 1968}, calcium phosphate coprecipitation (Graham and Van der Eb, 1973), direct transfer by bacterial protoplast fusion (Schaffner, 1980), microinjection (Y amamoto et al, 1982), liposome mediated transfer (Schaeffer-Ridder et al, 1982), retroviral vectors (Grober et al, 1985), Iaser-mediated transfer (Kurata et al, 1986) and electroinjection methods (Zimmermann, 1974a). Most of these methods were developed for use with adherent cells in monolayer culture and their efficiency is markedly reduced when they are applied to lymphoid cells is suspension (Rice and Baltimore, 1982;Oi et al, 1983).…”

Section: Discussionmentioning

confidence: 99%

Increased efficiency of transfection of murine hybridoma cells with DNA by electropermeabilization

Stopper

Zimmermann

Neil

1988

Journal of Immunological Methods

View full text Add to dashboard Cite

Dispase-treated murine hybridoma cells (SP2/0-Ag14) were transfected with the G418 resistance gene bearing plasmid pSV2-neo by electropermeabilization with a high degree of efficiency. The cells were subjected to intermittent multiple high-voltage short duration (5 p.s) DC pulses at intervals of 1 min in a weakly conducting medium followed by selection in G418-containing medium. The transfection medium, temperature, pulse duration, and voltage were empirically determined by preliminary electropermeabilization experiments. Increasing the number of pulses resulted in a higher percentage of transfected cells, but a decrease in the number of viable cells, with the optimal transfectant yield resulting when five pulses of 10 kV jcm were administered. This method allows the rapid and efficient injection of DNA into mammalian cells, and permits the rapid production of stable, drug resistant hybridoma celllines for use in subsequent fusion experiments.Key words: DNA transfection; Electropermeabilization; Eukaryotic cell; Hybridoma; Drug resistance IntroducnonThe injection of foreign DNA into eukaryotic cells, and its subsequent expression in these cells is a powerful research tool with wide application in cellular and molecular biology. Using this technique, it is possible to rapidly render otherwise susceptible cell lines resistant to antibiotics by injection and subsequent genomic integration and expression of bacterial antibiotic resistance genes. One important use for such drug resistant cells is the production of 'fusomas' or hybrid hybridomas (Milstein et al., 1983;Semenenko et al.;1985, Correspondence to: G.A. Neil, Ludwig Institute for Cancer Research, Toronto M4Y 1 M4, Canada.• This work was supported by grants from the BMFT, the DFLVR, the Deutsche Forschungsgemeinschaft (SFB 176), and the Medical Research Council of Canada. Suresh et al., 1986) which may then be conveniently selected after somati~ cell hybridization in appropriate antibiotic-containing medium (Lanzavecchia et al., 1987). A number of such drug resistance genes, incorporated in weil characterized plasmid vectors are now readily available and functional in mammalian cells after integration.We have recently refined and optimized the methodology for insertion of the plasmid vector containing the bacterial drug resistance gene, neomycin phosphotransferase (pSV2-neo) (Southem and Berg, 1982) into the non-secreting mammalian hybridoma cell line, SP2/0-Agl4 (Sp2/0) by means of electropermeabilization, thus en_. abling the selection of transfectants in growth medium supplemented with the antibiotic G418.The crucial step in this method is the production of reversible electrical breakdown of the cell

show abstract

Liposomes as Gene Carriers: Efficient Transformation of Mouse L Cells by Thymidine Kinase Gene

Cited by 153 publications

References 14 publications

Shock wave permeabilization as a new gene transfer method

Shock wave permeabilization as a new gene transfer method

Protection against oxygen toxicity by intravenous injection of liposome-entrapped catalase and superoxide dismutase.

Increased efficiency of transfection of murine hybridoma cells with DNA by electropermeabilization

Contact Info

Product

Resources

About