Liposomes containing Antiviral Antibody can protect Cells from Virus Infection

Magee, Wayne E.; Miller, Olga V.

doi:10.1038/235339a0

Cited by 73 publications

(25 citation statements)

References 11 publications

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“…For instance, stearylamine liposomes are quantitatively attached to cells in culture in the absence of serum, presumably as a result of electrostatic attraction [16], but fail to do so in the presence of serum (our unpublished observations). Indeed, the absence of an enhanced uptake in vivo of stearylamine liposomes by the liver and spleen or by circulating cells, could be the result of neutralization of such liposomes by negatively charged plasma constituents.…”

Section: The Efiect Of the Surface Charge Of Liposomes On Their Elimimentioning

confidence: 99%

“…4A). It is known that some liposome-entrapped proteins are partially arranged at or near the surface, interdigitated with the lipids [16,22], and it is therefore possible that glutaraldehyde treatment of albumincontaining liposomes led to their aggregation through cross-linking of the projected parts of albumin molecules, and to an increased rate of clearance presumably by the tissues of the reticuloendothelial system.…”

Section: The Effect Of Cross-linking Of Liposome-en Trapped Proteins mentioning

confidence: 99%

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Control of the Rate of Hepatic Uptake and Catabolism of Liposome‐Entrapped Proteins Injected into Rats. Possible Therapeutic Applications

Gregoriadis

Neerunjun

1974

European Journal of Biochemistry

203

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I3'I-or '251-labelled albumin and B-fructofuranosidase were entrapped in liposomes composed of phosphatidylcholine, cholesterol and a charged lipid (molar ratio 7 : 2 : 1). Investigations on the fate of such liposomes injected into rats revealed the following.1. Initially there is an increased rate of removal from plasma (rapid phase) followed by a decreased one (slow phase). The rate of removal during the rapid phase was highest for negatively charged liposomes and lowest for positively charged liposomes with neutral liposomes in between. Uniform rates of removal, regardless of charge, were obtained during the slow phase.2. The rate of hepatic uptake of such liposomes paralleled that observed for their removal from plasma during the rapid phase.3. Concurrent administration of a large quantity of positively charged liposomes and of a smaller quantity of negatively charged liposomes led to a diminution of the rapid phase of the latter suggesting a common site of uptake for liposomes of either charge.4. Blockade of the reticuloendothelial system with carbon led to an increased hepatic localization of liposomes during the rapid phase suggesting a bimodal uptake of liposomes with both the Kupffer and the liver parenchymal cells involved.5. Cross-linking of liposome-entrapped albumin and B-fructofuranosidase increased the stability of the latter and following injection into rats rendered both proteins less vulnerable to degradation by the liver lysosomal enzymes.6. The possibility of controlling both the rate of removal from plasma and catabolism of liposomeentrapped proteins adds more scope to the use of liposomes in therapy.

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Section: The Efiect Of the Surface Charge Of Liposomes On Their Elimimentioning

confidence: 99%

Section: The Effect Of Cross-linking Of Liposome-en Trapped Proteins mentioning

confidence: 99%

Control of the Rate of Hepatic Uptake and Catabolism of Liposome‐Entrapped Proteins Injected into Rats. Possible Therapeutic Applications

Gregoriadis

Neerunjun

1974

European Journal of Biochemistry

203

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“…However, positively charged stearylamine liposomes were reported to be toxic either in cell culture systems (Magee & Miller 1972;Campbell 1983;Mayhew et a1 1987)) or in-vivo. Most of the liposome publications which outlined the animal toxicity of positively charged stearylamine liposomes referred to the work of Adams et a1 (1977).…”

mentioning

confidence: 99%

Physicochemical Characterization and Acute Toxicity Evaluation of a Positively-charged Submicron Emulsion Vehicle

Klang

Frucht‐Pery

Hoffman

et al. 1994

Journal of Pharmacy and Pharmacology

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Fine, homogeneous, positively-charged emulsions with a mean droplet size of 138 +/- 71 nm and a zeta potential value of 41.06 mV were prepared using a combination of emulsifiers comprising phospholipids, poloxamer 188, and stearylamine. The pH of these emulsions decreased with time. However, the extent of decrease was dependent on the storage temperature. The mean droplet size of the emulsions that had been prepared with 1% poloxamer began to increase slightly after six months' storage, particularly when stored at 23 and 37 degrees C. However, emulsions prepared with 2% poloxamer remained stable for at least 10 months at 4 degrees C, suggesting that the poloxamer 188 concentration is critical for prolonged emulsion stability. The results of the ocular tolerance study in rabbit eye indicate that hourly administration of a positively-charged emulsion vehicle was well tolerated without any toxic or inflammatory response to the ocular surface during the five days of the study. Scanning electron microscopy revealed a normal corneal surface, which was not different from that of the animals treated with physiological saline. No marked acute toxicity was observed when 0.6 mL of positively-charged emulsion was injected intravenously to BALB/c mice. Furthermore, no difference was noted between this group of animals and the group injected with the marketed Intralipid emulsion. These results were further confirmed in a rat study where there were no deaths following intravenous injection of 3.3 mL per rat of the positively-charged emulsion or Intralipid. Neither emulsion elicited any hepatotoxic or nephrotoxic effects. The overall results suggest that the novel positively-charged emulsion is suitable for parenteral use, and for ocular application.

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“…A wide variety of liposome-associated enzymes, drugs and other agents has been successfully applied in model systems for enzyme replacement therapy [2][3][4], cancer chemotherapy [5][6][7], heavy metal detoxification [8], prevention of viral infections [9] and also as immunological adjuvants [10,11 ], interferon inducers [12] and diagnostic agents [13]. In addition, liposomes have been used for the introduction into cells of agents which, normally, have no intracellular access [ 14,15 ].…”

Section: Introductionmentioning

confidence: 99%

Penetration of target areas in the rat by liposome‐associated bleomycin, glucose oxidase and insulin

1976

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Liposomes containing Antiviral Antibody can protect Cells from Virus Infection

Cited by 73 publications

References 11 publications

Control of the Rate of Hepatic Uptake and Catabolism of Liposome‐Entrapped Proteins Injected into Rats. Possible Therapeutic Applications

Control of the Rate of Hepatic Uptake and Catabolism of Liposome‐Entrapped Proteins Injected into Rats. Possible Therapeutic Applications

Physicochemical Characterization and Acute Toxicity Evaluation of a Positively-charged Submicron Emulsion Vehicle

Penetration of target areas in the rat by liposome‐associated bleomycin, glucose oxidase and insulin

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