Liposomes Containing Zinc-Based Chemotherapeutic Drug Block Proliferation and Trigger Apoptosis in Breast Cancer Cells

Siva, Mallayasamy; Das, Kiran; Guha, Subhabrata; Sivagnanam, Subramaniyam; Das, Gaurav; Saha, Abhijit; Stewart, Adele; Maity, Biswanath; Das, Priyadip

doi:10.1021/acsabm.3c00587

Cited by 4 publications

(4 citation statements)

References 56 publications

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“…To assess the anti-migratory potential of RTME, wound wound-healing assay was performed following a previously established protocol (Siva et al, 2023) with few modifications. In brief, 2 × 10 5 cells/well were cultured overnight in serum-free media to reduce cellular proliferation and treated with 11.9 µg/mL and 23.8 µg/mL concentrations of RTME.…”

Section: Methodsmentioning

confidence: 99%

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Crude extract ofRuellia tuberosaL. flower induces intracellular ROS, promotes DNA damage and apoptosis in Triple Negative Breast Cancer Cells

Guha,

Talukdar,

Mandal

et al. 2024

Preprint

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Ethnophamacological relevance: In the traditional folklore medicine system, the primary uses of Ruellia tuberosa L. include as a diuretic, anti-hypertensive, antipyretic, anti-diabetic, analgesic, and gastroprotective agent. Some reports also demonstrated that it has been used to treat gonorrhea-like diseases. Purpose: Exploring the anti-cancer potential of the methanolic extract of Ruellia tuberosa L. flower (RTME) with special emphasis on human triple-negative breast cancer (TNBC) and investigating the possible signaling networks and regulatory pathways underlying it. Methods: Preparation of RTME and identifying the possible phytochemicals through GC-MS analysis. The anti-cancer potential of RTME was executed through in-vitro cytotoxicity assay, clonogenic assay, wound healing assay, ROS generation assay, cell cycle arrest, apoptotic nuclear morphology study, cellular apoptosis study, mitochondrial membrane potential (MMP) alteration study, protein and gene expressions alteration study. Apart from this, toxicological status and in-silico molecular docking studies were also conducted. Results: From this study, it was obvious that several phytochemicals within RTME have the potential to act as anti-cancerous agents. RTME was found to exhibit significant in-vitro cytotoxicity along with a reduction in colony formation and inhibition of cell migratory potential in MDA-MB-231 cells. RTME also induced intracellular ROS, promoted G0/G1 cell cycle arrest, caused mitochondrial membrane potential (MMP) alteration, and promoted cell death. From the pro- and anti-apoptotic marker study through the western blot and the qRT-PCR analysis, it was revealed that RTME promoted the intrinsic pathway of apoptosis. Furthermore, blood parameters and histological analysis revealed that RTME does not exhibit any toxic effect on female Balb/C mice. Finally, an in-silico molecular docking study revealed that the three identified lead phytochemicals in RTME show strong receptor-ligand interactions with the anti-apoptotic Bcl-2 and give a clue to the possible molecular mechanism of the RTME extract. Conclusion: From the findings, it was concluded that RTME has a significant therapeutic potential against TNBC which could be an alternative option for anti-cancer drug development.

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Section: Methodsmentioning

confidence: 99%

“…To assess the anti-migratory potential of RTME, wound wound-healing assay was performed following a previously established protocol (Siva et al, 2023) with few modifications.…”

Section: Wound Healing Assaymentioning

confidence: 99%

Crude extract ofRuellia tuberosaL. flower induces intracellular ROS, promotes DNA damage and apoptosis in Triple Negative Breast Cancer Cells

Guha,

Talukdar,

Mandal

et al. 2024

Preprint

Self Cite

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show abstract

“…In order to protect the active components of drugs, enhance therapeutic efficacy, improve drug bioavailability and solubility, and deliver therapeutic agents selectively to their target sites, several drug delivery systems have been established [1–3] . Liposome‐based drug delivery is a convenient and useful approach due to non‐toxicity, biocompatibility and biodegradability [4–12] . The plasma membrane in a human cell acts as a stable separating barrier between the cytoplasmic components and the extracellular medium of the cell.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3] Liposome-based drug delivery is a convenient and useful approach due to non-toxicity, biocompatibility and biodegradability. [4][5][6][7][8][9][10][11][12] The plasma membrane in a human cell acts as a stable separating barrier between the cytoplasmic components and the extracellular medium of the cell. In addition, it is an important biological interface where crucial biological reactions for cell functions take place.…”

Section: Introductionmentioning

confidence: 99%

Liposome‐Based Delivery of a Bis‐benzimidazole Derivative to Duplex DNA: A Spectroscopic Study

Shit,

Mandal,

Das

et al. 2024

ChemistrySelect

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Due to the immense pharmacological significance, the interaction of small molecules with liposome and the exact knowledge of their binding location and distribution within the lipid bilayer of liposome is an active field of biophysical research. Here, we have studied the binding interaction of H33258 with DPPC liposome, a model biological membrane. From the steady‐state fluorescence spectroscopy it was revealed that H33258 bounded to DPPC liposome and the estimated binding constant value was 2.5×102 M−1. It was also revealed that the H33258 essentially partitioned within the lipid bilayer of DPPC liposome. Moreover, from steady‐state fluorescence, time‐resolved lifetime, time‐resolved anisotropy and ITC studies it was established that the probe molecules were sequestrated from the lipid bilayer and bounded to a duplex DNA structure. Our results on the binding consequences of H33258 with liposome will aid development of a simple and safe strategy to deliver drugs to the target sites using liposome, endogenously.

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Crude extract of Ruellia tuberosa L. flower induces intracellular ROS, promotes DNA damage and apoptosis in triple negative breast cancer cells

Guha,

Talukdar,

Mandal

et al. 2024

Journal of Ethnopharmacology

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Liposomes Containing Zinc-Based Chemotherapeutic Drug Block Proliferation and Trigger Apoptosis in Breast Cancer Cells

Cited by 4 publications

References 56 publications

Crude extract ofRuellia tuberosaL. flower induces intracellular ROS, promotes DNA damage and apoptosis in Triple Negative Breast Cancer Cells

Crude extract ofRuellia tuberosaL. flower induces intracellular ROS, promotes DNA damage and apoptosis in Triple Negative Breast Cancer Cells

Liposome‐Based Delivery of a Bis‐benzimidazole Derivative to Duplex DNA: A Spectroscopic Study

Crude extract of Ruellia tuberosa L. flower induces intracellular ROS, promotes DNA damage and apoptosis in triple negative breast cancer cells

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