Liposomes for enhanced cytotoxic activity of bleomycin

Alomrani, Abdullah H.; Maghraby, Gamal M. El; Alanazi, Fars K.; Al-Mohanna, Mai; Alaiya, Ayodele; Alsarra, Ibrahim A.

doi:10.1002/ddr.20394

Cited by 17 publications

(8 citation statements)

References 65 publications

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“…[23,24] As expected, encapsulation and loading efficiencies obtained were significantly higher than those previously reported. [25,26] High entrapment efficiency, proper stability, and sustained release behavior which were observed in this study confirmed that the preparation process of nanoparticles was optimal. These achievements are probably related to the liposome components and their correct amounts.…”

Section: Discussionsupporting

confidence: 73%

“…Their size and shape are appropriate for use a carrier system for drug delivery because the optimum size to evade of phagocytes and penetrate to the tumor cells should be lower than 200 nm . As expected, encapsulation and loading efficiencies obtained were significantly higher than those previously reported …”

Section: Discussionmentioning

confidence: 99%

“…Alomrani et al [25] investigated the effect of liposome composition on the cytotoxic efficacy of bleomycin. The cytotoxic efficacy was investigated against Daudi cell line derived from Burkitt's lymphoma.…”

Section: Preparation Characterization and In Vitro Evaluation Of Blmentioning

confidence: 99%

“…Alomrani et al . investigated the effect of liposome composition on the cytotoxic efficacy of bleomycin.…”

Section: Introductionmentioning

confidence: 99%

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Preparation, characterization, and in vitro evaluation of bleomycin‐containing nanoliposomes

et al. 2016

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Bleomycin is an anticancer drug used against various types of cancers. The aim of this study was to prepare a new PEGylated and non-PEGylated nanoliposomal formulation of bleomycin (PEG-nLip-BLM and nLip-BLM) and evaluate their anticancer activity in different tumor cell lines. The liposomes were prepared by thin-film hydration method, and then, bleomycin (BLM) was loaded to the prepared vesicles. The size, zeta potential, entrapment efficiency, loading rate, release profile, and cytotoxicity of liposomal formulations in TC-1, LLC1, and HFLF-PI5 cell lines were investigated. Mean particle size and zeta potential of the PEG-nLip-BLM and nLip-BLM were found to be 99.4 ± 4.6 nm and -34.83 ± 4.7 mV; and 112.2 ± 7.2 nm and -27.5 ± 3.2 mV, respectively, which were stable for at least 2 months. Encapsulation and loading efficiency of BLM for PEG-nLip-BLM and nLip-BLM were obtained about 83.1 ± 4.2% and 14.3 ± 2.5%; and 78.3 ± 8.6% and 11.1 ± 3.3%, respectively. Drug release study showed a slow release pattern without considerable burst effect. The liposomal formulations indicated lower toxicity compared to free drug in case of TC-1 and HFLF-PI5 cells, but their cytotoxicity against LLC1 cells was significantly higher than free drug. The results of this study indicated that PEG-nLip-BLM can be a suitable candidate for drug delivery to solid tumors.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Preparation Characterization and In Vitro Evaluation Of Blmentioning

confidence: 99%

“…Alomrani et al . investigated the effect of liposome composition on the cytotoxic efficacy of bleomycin.…”

Section: Introductionmentioning

confidence: 99%

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Preparation, characterization, and in vitro evaluation of bleomycin‐containing nanoliposomes

et al. 2016

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“…It has been shown that bleomycin is a drug with relatively low permeability and minimal intracellular concentrations. 7 , 8 Therefore, the rate-limiting step of the sclerosant effect of bleomycin lies in its membrane permeability. Alternative strategies have been investigated to enhance the permeability of drugs with low permeability through biological membranes.…”

Section: Introductionmentioning

confidence: 99%

Vascular sclerosing effects of bleomycin on cutaneous veins: a pharmacopathologic study on experimental animals

AlGhamdi

Kumar

Ashour

et al. 2017

An. Bras. Dermatol.

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BackgroundVaricose veins and the complications of venous disease are common disorders in humans.ObjectiveTo study the effects of bleomycin as a potential new sclerosing agent and its adverse events in treating varicose veins.MethodsBleomycin-loaded liposomes 0.1ml was injected in the dorsal ear veins of white New Zealand rabbits. Sodium tetradecyl sulfate was used as a positive control. Normal saline was used as negative control. The blood vessels of the treated ears were photographed before and at one hour and two, eight and 45 days after treatment. Biopsies from the treated areas were obtained for histological examination. Blood samples were collected to determine any possible toxicity.ResultsBleomycin by itself was ineffective; therefore, liposomes were used as a vector to deliver bleomycin to the vein lumen. Subsequently, bleomycin started showing its sclerosing effects. Toxicity monitoring showed no apparent hematologic, pulmonary, hepatic or renal toxicities. This study revealed that bleomycin induced vasculitis, which led to vascular occlusion, which was observed on day 1 and day 8. No bleomycin-related injury was noted by histopathological examination of lung sections. The calculation of the lung/body weight coefficient indicated that edema was present in the experimental groups compared with the negative and positive controls.Study limitationsRelatively small number of experimental animals used.ConclusionsThis study showed that bleomycin-loaded liposomes were able to induce vasculitis and vascular occlusion without any toxicity or complications. It might be useful, hence, to treat patients suffering from Varicose veins and other ectatic vascular diseases with this agent.

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Bleomycin sulphate loaded nanostructured lipid particles augment oral bioavailability, cytotoxicity and apoptosis in cervical cancer cells

Saini

Bansal

Chandra

et al. 2014

Colloids and Surfaces B: Biointerfaces

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Liposomes for enhanced cytotoxic activity of bleomycin

Cited by 17 publications

References 65 publications

Preparation, characterization, and in vitro evaluation of bleomycin‐containing nanoliposomes

Preparation, characterization, and in vitro evaluation of bleomycin‐containing nanoliposomes

Vascular sclerosing effects of bleomycin on cutaneous veins: a pharmacopathologic study on experimental animals

Bleomycin sulphate loaded nanostructured lipid particles augment oral bioavailability, cytotoxicity and apoptosis in cervical cancer cells

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