Liposomes in tissue engineering and regenerative medicine

Monteiro, Nelson; Martins, Albino; Reis, Rui L.; Neves, Nuno M.

doi:10.1098/rsif.2014.0459

Cited by 327 publications

(262 citation statements)

References 188 publications

(394 reference statements)

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“…A successful treatment using drug-loaded liposomes depends also on the route of administration (e.g., s.c., perorally, and i.v.). Conventional liposomes, administered s.c., aim to target the lymphatic system for the distribution of therapeutic agents or vaccination (24). Liposomes administered i.v.…”

Section: Discussionmentioning

confidence: 99%

Nanoliposomal Buparvaquone Immunomodulates Leishmania infantum-Infected Macrophages and Is Highly Effective in a Murine Model

Costa-Silva

Galisteo

Lindoso

et al. 2017

Antimicrob Agents Chemother

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Visceral leishmaniasis is a fatal parasitic neglected disease affecting 1.5 million people worldwide. Based on a drug repositioning approach, the aim of this work was to investigate the in vitro immunomodulatory potential of buparvaquone (BPQ) and to establish a safe regimen to evaluate the in vivo efficacy of BPQ entrapped by negatively charged nanoliposomes (BPQ-LP) in Leishmania infantum-infected hamsters. Small-angle X-ray scattering, dynamic light scattering, and the -potential were applied in order to study the influence of BPQ on the liposome structure. Our data revealed that BPQ was located in the polar-apolar interface, snorkeling the polar region, and protected against aggregation inside the lipophilic region. The presence of BPQ also decreased the Z-average hydrodynamic diameter and increased the surface charge. Compared to intravenous and intramuscular administration, a subcutaneous route was a more effective route for BPQ-LP; at 0.4 mg/kg, BPQ-LP reduced infection in the spleen and liver by 98 and 96%, respectively. Treatment for 5 days resulted in limited efficacy, but 10 days of treatment resulted in an efficacy similar to that of a 15-day regimen. The nanoliposomal drug was highly effective, with a mean 50% effective dose of 0.25 mg/kg, reducing the parasite load in bone marrow by 80%, as detected using quantitative PCR analysis. In addition, flow cytometry studies showed that BPQ upregulated cytokines as tumor necrosis factor, monocyte chemoattractant protein 1, interleukin-10 (IL-10), and IL-6 in Leishmania-infected macrophages, eliminating the parasites via a nitric oxide-independent mechanism. This new formulation proved to be a safe and effective treatment for murine leishmaniasis that could be a useful candidate against visceral leishmaniasis.KEYWORDS Leishmania, drug delivery, liposomes, therapy T he worldwide prevalence and severity of leishmaniasis has led the World Health Organization to consider it a priority infectious disease (1). Due to the complexity of host-parasite interaction, as well as contradictory results from vaccination models, the control of infection is mainly dependent on chemotherapeutic intervention (2). There are now a number of therapies for various forms of leishmaniasis; the preferences for first-line and second-line treatment vary based on the type of disease and are often guided by regional practice. Pentavalent antimonials, such as Pentostam and Glucantime, have been used to treat leishmaniasis for the last 70 years (3). However, these drugs have multiple adverse effects and decreasing effectiveness due to the development of parasite resistance, limiting their application (4). In Brazil, pentavalent antimony is still effective for treating most infections, despite its high toxicity. Until now, Citation da Costa-Silva TA, Galisteo AJ, Jr, Lindoso JAL, Barbosa LRS, Tempone AG. 2017. Nanoliposomal buparvaquone immunomodulates Leishmania infantuminfected macrophages and is highly effective in a murine model. Antimicrob Agents

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Section: Discussionmentioning

confidence: 99%

Nanoliposomal Buparvaquone Immunomodulates Leishmania infantum-Infected Macrophages and Is Highly Effective in a Murine Model

Costa-Silva

Galisteo

Lindoso

et al. 2017

Antimicrob Agents Chemother

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“…5,6 They are successfully used for a number of biomedical purposes: from model systems to study biological membranes to efficient vectoring carriers for delivering bioactive substances or other molecules to pathological sites. [7][8][9] The need for systematic approaches to optimize specific membrane physico-chemical characteristics (e.g. mechanical resistance, wall thickness, chemical groups exposed on the outer surface) has driven the search for new categories of amphiphiles other than phospholipids.…”

Section: Introductionmentioning

confidence: 99%

Novel stable dendrimersome formulation for safe bioimaging applications

et al. 2015

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a Dendrimersomes are nanosized vesicles constituted by amphiphilic Janus dendrimers (JDs), which have been recently proposed as innovative nanocarriers for biomedical applications. Recently, we have demonstrated that dendrimersomes self-assembled from (3,5)12G1-PE-BMPA-G2-(OH) 8 dendrimers can be successfully loaded with hydrophilic and amphiphilic imaging contrast agents. Here, we present two newly synthesized low generation isomeric JDs: JDG0G1(3,5) and JDG0G1(3,4). Though less branched than the above-cited dendrimers, they retain the ability to form self-assembled, almost monodisperse vesicular nanoparticles. This contribution reports on the characterization of such nanovesicles loaded with the clinically approved MRI probe Gadoteridol and the comparison with the related nanoparticles assembled from more branched dendrimers. Special emphasis was given to the in vitro stability test of the systems in biologically relevant media, complemented by preliminary in vivo data about blood circulation lifetime collected from healthy mice. The results point to very promising safety and stability profiles of the nanovesicles, in particular for those made of JDG0G1(3,5), whose spontaneous self-organization in water gives rise to a homogeneous suspension. Importantly, the blood lifetimes of these systems are comparable to those of standard liposomes. By virtue of the reported results, the herein presented nanovesicles augur well for future use in a variety of biomedical applications.

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“…Liposomes have inner aqueous environment and outer hydrophobic moieties that rendered them as an excellent carrier for drug delivery [76]. It has been seen that covering of outermost envelope structure of multi-lamellar vesicles with surface layer proteins enabled the well-defined orientation, spatial distribution of target molecules resembling the "artificial cell envelopes" or "artificial virus-like particles" [77].…”

Section: ) Liposomesmentioning

confidence: 99%

Bacterial Surface Layer Proteins: From Moonlighting to Biomimetics: A New Horizonto Lead

Gaur¹,

Sharma²,

Singhal³

2018

ABB

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The landmark discovery of moonlighting proteins embarks the significant progress in understanding the biological complexity and their closed-circuit analysis. The growing continuum in the variety of moonlighting functions paved the way for further elucidation of structural-functional aspects of protein evolution and design of proteins with novel functions. Currently, the moonlighting functions in various adhesive properties of surface layer proteins, an essential component of cell surface architecture of archaea and all phylogenetic groups of eubacteria become more prominently recognized. The remarkable credentials of surface layer proteins to self-assemble into supramolecular structures at nano-scale dimension have been exploited for the production of smart biomaterials in the form of biomimetics has been thrust area of research. The finely tuned topological features in terms of shape, size, geometry and surface chemistry of surface layer proteins are crucial for the production of biomimetics. The current developments of biomimetic lipid bilayers and composite membranes find applicability in understanding the functional dynamism of evolutionary relationship of bacterial cell envelopes and vaccine development, drug development and drug delivery. Though the development of biomimetics embraces fascination but faces with technological challenges. The plethora of literature has been available for the moonlighting aspects and nano-technological applications separately but none of the review describes towards the rhythmic transition from moonlighting functions of surface layer proteins of bacteria to biomimetics development and applications. Therefore, this review describes certain basic aspects of moonlighting functions and their mechanism of action, surface layer proteins and their moonlighting functions of commensal bacteria and their transition towards biomimetics. The recent developments of biomimetics based on surface layer proteins have been summarized and also posited different chal-

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Liposomes in tissue engineering and regenerative medicine

Cited by 327 publications

References 188 publications

Nanoliposomal Buparvaquone Immunomodulates Leishmania infantum-Infected Macrophages and Is Highly Effective in a Murine Model

Nanoliposomal Buparvaquone Immunomodulates Leishmania infantum-Infected Macrophages and Is Highly Effective in a Murine Model

Novel stable dendrimersome formulation for safe bioimaging applications

Bacterial Surface Layer Proteins: From Moonlighting to Biomimetics: A New Horizonto Lead

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