Lipoxygenase-Catalyzed Phospholipid Peroxidation: Preparation, Purification, and Characterization of Phosphatidylinositol Peroxides

Butler, Elizabeth Susan O’Connor; Mazerik, Jessica N.; Cruff, Jason; Sherwani, Shariq I.; Weis, Barbara K.; Marsh, Clay B.; Raghavamenon, Achuthan C.; Uppu, Rao M.; Schmid, Harald H.O.; Parinandi, Narasimham L.

doi:10.1007/978-1-60327-029-8_23

Cited by 6 publications

(2 citation statements)

References 15 publications

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“…However, marked repression of cardiac genes related to compensatory responses and structural components, as well as mitochondrial aconitase activity, in that study prompted us to examine the effect of ozone and DEP on composition of fatty acid phospholipids as an index of oxidative stress in the myocardial mitochondria. PUFA concentrations were decreased in myocardial mitochondria after exposure to ozone and DEP alone, likely due to phospholipase-mediated or nonenzymatic oxidation (Jin and Zhou 2009; O’Connor Butler et al 2010). The loss of phospholipids might impair contractile response of the myocardium.…”

Section: Discussionmentioning

confidence: 99%

Vascular and Cardiac Impairments in Rats Inhaling Ozone and Diesel Exhaust Particles

Kodavanti

Thomas

Ledbetter

et al. 2011

Environ Health Perspect

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BackgroundMechanisms of cardiovascular injuries from exposure to gas and particulate air pollutants are unknown.ObjectiveWe sought to determine whether episodic exposure of rats to ozone or diesel exhaust particles (DEP) causes differential cardiovascular impairments that are exacerbated by ozone plus DEP.Methods and resultsMale Wistar Kyoto rats (10–12 weeks of age) were exposed to air, ozone (0.4 ppm), DEP (2.1 mg/m3), or ozone (0.38 ppm) + DEP (2.2 mg/m3) for 5 hr/day, 1 day/week for 16 weeks, or to air, ozone (0.51 or 1.0 ppm), or DEP (1.9 mg/m3) for 5 hr/day for 2 days. At the end of each exposure period, we examined pulmonary and cardiovascular biomarkers of injury. In the 16-week study, we observed mild pulmonary pathology in the ozone, DEP, and ozone + DEP exposure groups, a slight decrease in circulating lymphocytes in the ozone and DEP groups, and decreased platelets in the DEP group. After 16 weeks of exposure, mRNA biomarkers of oxidative stress (hemeoxygenase-1), thrombosis (tissue factor, plasminogen activator inhibitor-1, tissue plasminogen activator, and von Willebrand factor), vasoconstriction (endothelin-1, endothelin receptors A and B, endothelial NO synthase) and proteolysis [matrix metalloprotease (MMP)-2, MMP-3, and tissue inhibitor of matrix metalloprotease-2] were increased by DEP and/or ozone in the aorta, but not in the heart. Aortic LOX-1 (lectin-like oxidized low-density lipoprotein receptor-1) mRNA and protein increased after ozone exposure, and LOX-1 protein increased after exposure to ozone + DEP. RAGE (receptor for advanced glycation end products) mRNA increased in the ozone + DEP group. Exposure to ozone or DEP depleted cardiac mitochondrial phospholipid fatty acids (DEP > ozone). The combined effect of ozone and DEP exposure was less pronounced than exposure to either pollutant alone. Exposure to ozone or DEP for 2 days (acute) caused mild changes in the aorta.ConclusionsIn animals exposed to ozone or DEP alone for 16 weeks, we observed elevated biomarkers of vascular impairments in the aorta, with the loss of phospholipid fatty acids in myocardial mitochondria. We conclude that there is a possible role of oxidized lipids and protein through LOX-1 and/or RAGE signaling.

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Section: Discussionmentioning

confidence: 99%

Vascular and Cardiac Impairments in Rats Inhaling Ozone and Diesel Exhaust Particles

Kodavanti

Thomas

Ledbetter

et al. 2011

Environ Health Perspect

Self Cite

100

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“…The lipid component contains major classes of phospholipids, including phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidic acid, phosphatidylglycerol, and cardiolipin [ 53 ]. As well as cardiolipin, most of these lipids are capable of peroxidation, such as phosphatidylcholine [ 54 , 55 ], phosphatidylethanolamine [ 56 , 57 ], phosphatidylinositol [ 58 ], and phosphatidylserine [ 59 ]. Phosphatidic acid, conversely, showed an inhibitory effect on lipid peroxidation [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

Composition of the Cytochrome c Complex with Cardiolipin by Thermal Lens Spectrometry

et al. 2023

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Thermal lens spectrometry along with spectrophotometric titration were used to assess the composition of the complex of oxidized cytochrome c (ferricytochrome c) with 1,1′,2,2′-tetraoleyl cardiolipin, which plays a key role in the initiation of apoptosis. Spectrophotometric titration was carried out for micromolar concentrations at which the complex is mainly insoluble, to assess the residual concentration in the solution and to estimate the solubility of the complex. Thermal lens spectrometry was used as a method of molecular absorption spectroscopy, which has two advantages over conventional optical transmission spectroscopy: the higher sensitivity of absorbance measurements and the possibility of studying the light absorption by chromophores and heat transfer in complex systems, such as living cells or tissues. Thermal lens measurements were carried out at nanomolar concentrations, where the complex is mainly in solution, i.e., under the conditions of its direct measurements. From the thermal lens measurements, the ratios of cytochrome c and cardiolipin in the complex were 50 at pH 7.4; 30 at pH 6.8; and 10 at pH 5.5, which fit well to the spectrophotometric data. The molecular solubility of the complex at pH 6.8–7.4 was estimated as 30 µmol/L.

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Profiling and Qualitative Assessment of Enzymatically and Thermally Oxidized Egg Yolk Phospholipids using a Two‐Step High‐Performance Liquid Chromatography Protocol

Parchem

Kusznierewicz

Chmiel

et al. 2019

J Americ Oil Chem Soc

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A two-step high-performance liquid chromatography (HPLC) method for the profiling and qualitative assessment of oxidized phospholipids (oxPL) present in foods was developed. The applicability of the investigated two-step HPLC protocol was verified for separation of enzymatically and thermally oxidized hen egg yolk phospholipids (PL) as a relevant food model. In the first step, seven individual PL classes were separated using hydrophilic interaction liquid chromatography (HILIC). The collected fractions of two main egg yolk PL classesphosphatidylethanolamines and phosphatidylcholineswere further directed to the second step of separation aimed at profiling and qualitative assessment of their oxidized species. For this purpose, the reverse phase (RP) chromatography coupled to charged aerosol, ultraviolet detection (UV) and mass spectrometry detection were employed. A database of potential oxPL including primary (hydroperoxides) and long-chain secondary PL oxidation products (epoxides, alcohols, and ketones) as well as some of their possible combinations was created. Additionally, the results were compared with the profiles of PL hydroperoxides obtained using thin layer chromatography (TLC) with N,N-dimethyl-p-phenylenediamine visualization.

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Lipoxygenase-Catalyzed Phospholipid Peroxidation: Preparation, Purification, and Characterization of Phosphatidylinositol Peroxides

Cited by 6 publications

References 15 publications

Vascular and Cardiac Impairments in Rats Inhaling Ozone and Diesel Exhaust Particles

Vascular and Cardiac Impairments in Rats Inhaling Ozone and Diesel Exhaust Particles

Composition of the Cytochrome c Complex with Cardiolipin by Thermal Lens Spectrometry

Profiling and Qualitative Assessment of Enzymatically and Thermally Oxidized Egg Yolk Phospholipids using a Two‐Step High‐Performance Liquid Chromatography Protocol

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