Lipoxygenase inhibitors from natural plant sources. Part 1: Medicinal plants with inhibitory activity on arachidonate 5‐lipoxygenase and 5‐lipoxygenase[sol ]cyclooxygenase

Schneider, Isabella; Bučar, Franz

doi:10.1002/ptr.1603

Cited by 118 publications

(105 citation statements)

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“…Negative correlation was found between the antioxidant activities and the total phenolic and flavonoids content (Table 5). properties the inhibition of 15-lipoxygenase pathway is considered to be interesting in the treatment of a variety of inflammatory diseases (Schneider and Bucar, 2005). The IC 50 values of extracts obtained in 15-LOX inhibitory activity are similar to those of other LOX active crude extracts in the range of 1-100µg/mL (Schneider and Bucar, 2005).…”

Section: Mesozygia E Magaliesmontanum L Leucocephala and L Jamentioning

confidence: 88%

“…properties the inhibition of 15-lipoxygenase pathway is considered to be interesting in the treatment of a variety of inflammatory diseases (Schneider and Bucar, 2005). The IC 50 values of extracts obtained in 15-LOX inhibitory activity are similar to those of other LOX active crude extracts in the range of 1-100µg/mL (Schneider and Bucar, 2005). Particularly Burkea africana with most potent activity (37.25±0.88 µg/mL) could be regarded as a potential source for new 15-LOX inhibitors.…”

Section: Mesozygia E Magaliesmontanum L Leucocephala and L Jamentioning

confidence: 96%

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Anti-inflammatory, anticholinesterase and antioxidant activity of leaf extracts of twelve plants used traditionally to alleviate pain and inflammation in South Africa

Dzoyem¹,

Eloff²

2015

Journal of Ethnopharmacology

140

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Ethnopharmacological relevanceOxidative stress and inflammatory conditions are among the pathological features associated with the central nervous system in Alzheimer's disease. Traditionally, medicinal plants have been used to alleviate inflammation, pains and also other symptoms possibly associated with Alzheimer's disease. Therefore, the present study was designed to determine the in vitro antiinflammatory, antioxidant and anticholinesterase activity of twelve South African medicinal plants traditionally used to alleviate pain and inflammation. Materials and MethodsNitric oxide (NO) production in LPS-activated RAW 264.7 macrophages and 15-lipoxygenase (LOX) inhibitory assay were used to evaluate the anti-inflammatory activity.Acetylcholinesterase inhibition was assessed by using a modification of the Ellman's method.Antioxidant activity, total phenolic and total flavonoids contents were determined using standard in vitro methods. ResultsThe extract of Burkea africana had the highest anti-15-lipoxygenase activity with 85.92% inhibition at 100 µg/mL. All the extracts tested inhibited nitric oxide (NO) production in a dose dependant manner in LPS-stimulated RAW 264.7 macrophages. However, extracts from Leucaena leucocephala, Lippia javanica inhibited the production of NO by 97% at a concentration of 25 µg/mL. In addition, both Leucaena leucocephala and Englerophytum magaliesmontanum had strong activity against acetylcholinesterase with IC 50 values of 118 µg/mL and 160 µg/mL respectively. Hight levels of phenolics and flavonoids were found in Leucaena leucocephala, Lippia javanica and Burkea africana. The correlation with antioxidant activities was not strong indicating that other metabolites may also be involved in antioxidant activity. ConclusionsThe results obtained in this study validate the use of leaf extracts of these plants in South African traditional medicine against inflammation. Extracts of these plants species might be of value in the management of various diseases emerging from oxidative stress and related degenerative disorders.2

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Section: Mesozygia E Magaliesmontanum L Leucocephala and L Jamentioning

confidence: 88%

Section: Mesozygia E Magaliesmontanum L Leucocephala and L Jamentioning

confidence: 96%

Anti-inflammatory, anticholinesterase and antioxidant activity of leaf extracts of twelve plants used traditionally to alleviate pain and inflammation in South Africa

Dzoyem¹,

Eloff²

2015

Journal of Ethnopharmacology

140

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“…Higher value of LOX activity IC 50 = 62.6 µg/ml with the bark of F. rhynchophylla has been found according to Huh et al (2015). Therefore, the selective inhibition of LOX is an important therapeutic strategy for asthma (Shah et al, 2011;Schneider and Bucar. 2005).…”

Section: Discussionmentioning

confidence: 99%

Antibacterial and Anti-Inflammatory Activities of Crude Extracts of Three Togolese Medicinal Plants Against Esbl Klebsiella Pneumoniae Strains

Toudji

Thiombiano

Karou³

et al. 2017

Afr J Tradit Complement Altern Med

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Background: Pithecellobium dulce (Roxb.) Benth., Securidaca longepedunculata Fresn and Cryptolepis sanguinolenta (Lindl.) Schlt are three plants widely used in the Togolese traditional medicine to treat microbial infections. Some studies reported their antibacterial activity alone but until know there no data concerning their possible interaction with conventional antibiotics. The main objective of this study was to investigate the antibacterial activity of the association of the crude extracts of the three plants with some conventional antibiotics. We further evaluate the antioxidant and the anti-inflammatory activities of the extracts on rat's model. Materials and methods:The antimicrobial activity was evaluated by the broth microdilution assay and the Fractional inhibitory concentrations (FIC) determined by the checkerboard method. The anti-inflammatory activity was evaluated using the Carrageenan-induced rat paw edema model. The antioxidant activities and the phenol contents were determined by spectrophotometry. Results: The MICs of hydroethanolic extract of plants ranged from 3.125 to 100 mg/mL on Klebsiella pneumoniae strains. Synergistic action was observed only with the combination of Imipenem/P. dulce, imipenem/C. sanguinolenta, amikacin/P. dulce and amikacin/C. sanguinolenta against the ESBL negative Klebsiella pneumoniae strain. Of the 21 associations, 15 were antagonistic on the ESBL-producing strains. The indifference effect was observed with the combination of the extract of Securidaca longepedunculata and the following antibiotics imipenem, amikacin, tetracyclin, ciprofloxacin, Cefotaxim; and Sulfametoxazol+Trimethoprim. The in vitro anti-inflammatory with Lipoxygenase inhibition activity was best with C. sanguinolenta extract while the in vivo paw edema model revealed that S. longepedunculata was the highest reducer of paw edema. In addition white blood cells count and biochemical parameters such as total proteins and immunoglobulins were significantly affected by the administration of plant extracts. Conclusion: This study revealed that the three plants although they may inhibit the bacterial growth by themselves, but there is also a possible synergistic action with the commercial antibiotics. Further investigations are needed to identify the active compounds and their mechanism of action.

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“…15-Lipoxygenase (15-LO) is an enzyme present in multiple systems and organs of the body that reacts with unsaturated fatty acids producing active lipid metabolites that are involved in a number of disease states such as cancer, psoriasis, atherosclerosis, and diabetes (both type 1 and 2) (Dobrian et al, 2011;Schneider & Bucar, 2005). For this reason, 15-LO has emerged as an attractive target for therapeutic intervention (Sadeghian et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

α-Glucosidase inhibition, 15-lipoxygenase inhibition, and brine shrimp toxicity of extracts and isolated compounds fromTerminalia macropteraleaves

Pham

Malterud

Paulsen

et al. 2014

Pharmaceutical Biology

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Context: Terminalia macroptera Guill. & Perr. (Combretaceae), a tree that grows in West Africa, has been used in traditional medicine against a variety of diseases such as hepatitis, gonorrhea, skin diseases, and diabetes. Objective: To investigate enzyme inhibitory activity against a-glucosidase and 15-lipoxygenase (15-LO) and toxicity against brine shrimp of extracts and compounds from T. macroptera leaves. Materials and methods: Methanol extract, ethyl acetate, and butanol extracts obtained from the methanol extract, six isolated polyphenols (chebulagic acid, chebulic acid trimethyl ester, corilagin, methyl gallate, narcissin, and rutin), and shikimic acid were evaluated for enzyme inhibition and toxicity. Results: In enzyme inhibition assays, all extracts showed high or very high activity. Chebulagic acid showed an IC 50 value of 0.05 mM towards a-glucosidase and 24.9 ± 0.4 mM towards 15-LO, in contrast to positive controls (acarbose: IC 50 201 ± 28 mM towards a-glucosidase, quercetin: 93 ± 3 mM towards 15-LO). Corilagin and narcissin were good 15-LO and a-glucosidase inhibitors, as well, while shikimic acid, methyl gallate, and chebulic acid trimethyl ester were less active or inactive. Rutin was a good a-glucosidase inhibitor (IC 50 ca. 3 mM), but less active towards 15-LO. None of the extracts or the isolated compounds seemed to be very toxic in the brine shrimp assay compared with the positive control podophyllotoxin. Conclusion: Inhibition of a-glucosidase in the gastrointestinal tract may be a rationale for the medicinal use of T. macroptera leaves against diabetes in traditional medicine in Mali. The plant extracts and its constituents show strong inhibition of the peroxidative enzyme 15-LO.

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Lipoxygenase inhibitors from natural plant sources. Part 1: Medicinal plants with inhibitory activity on arachidonate 5‐lipoxygenase and 5‐lipoxygenase[sol ]cyclooxygenase

Cited by 118 publications

References 118 publications

Anti-inflammatory, anticholinesterase and antioxidant activity of leaf extracts of twelve plants used traditionally to alleviate pain and inflammation in South Africa

Anti-inflammatory, anticholinesterase and antioxidant activity of leaf extracts of twelve plants used traditionally to alleviate pain and inflammation in South Africa

Antibacterial and Anti-Inflammatory Activities of Crude Extracts of Three Togolese Medicinal Plants Against Esbl Klebsiella Pneumoniae Strains

α-Glucosidase inhibition, 15-lipoxygenase inhibition, and brine shrimp toxicity of extracts and isolated compounds fromTerminalia macropteraleaves

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