Liquefied wheat bran as carbon source and inducer in high-solids submerged cultivation of Aspergillus niger for xylanase production

Amorim, Cristiane Costa; Farinas, Cristiane S.; Miranda, Everson Alves

doi:10.1016/j.bcab.2019.101346

Cited by 14 publications

(4 citation statements)

References 36 publications

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“…Thus, in addition to the cellulases, accessory enzymes are necessary to potentiate the degradation of the raw material, such as hemicellulases. Among the hemicellulases, xylanases act in the depolymerization of hemicelluloses and help in the conversion of biomass, contributing to the opening of the fibers and to the access of cellulases to cellulose (Amorim et al, 2019). Agrawal et al (2021), for example, produced D-feruloyl esterase and β-xylosidase by the fungus Scytalidium thermophilum and observed that the use of these homemade cocktails improved the hydrolysis of rice straw and sugarcane bagasse, demonstrating potential to reduce the load of commercial enzymes, and consequently, the cost of the sugars production from agricultural wastes.…”

Section: Influence Of the Enzymatic Synergismmentioning

confidence: 99%

Factors that influence the enzymatic hydrolysis of agricultural wastes for ethanol production: a review

Santos

Pasquini²,

Baffi³

2022

J. Eng. Exact Sci.

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Lignocellulosic biomass, such as agricultural and forestry residues, can be reused and serve as sources of sugars for the production of second-generation ethanol (2G) and other bioproducts. However, these wastes are composed by molecules of difficult degradation, which require steps of pretreatment and enzymatic hydrolysis for their bioconversion into fermentable sugars. At the same time, chemical substances with a potential inhibitory effect on the microbial metabolism can also be produced after the pretreatments and hinder the overall yield of the hydrolytic process. For an efficient and low-cost hydrolysis, homemade enzymes produced from agroindustrial residues, such as sugarcane bagasse, can be employed. However, a set of parameters might be adjusted, such as: kind of pretreatment, enzyme load, solids load, hydrolysis time and use of additives, to improve the yields in free sugars using these onsite enzymatic preparations. In this sense, studies involving the optimization of the conditions of pretreatment and saccharification are essential to increase the bioconversion rate of lignocellulose. These strategies are important for the production of value-added products from these wastes and, consequently, offer a correct and profitable destination to them. Hence, this study presents a review of the main features that influence the enzymatic hydrolysis of agricultural wastes and the yield in reducing sugars for ethanol production.

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Section: Influence Of the Enzymatic Synergismmentioning

confidence: 99%

Factors that influence the enzymatic hydrolysis of agricultural wastes for ethanol production: a review

Santos

Pasquini²,

Baffi³

2022

J. Eng. Exact Sci.

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“…AMPs are among such new agents [14][15][16], usually produced via chemical synthesis but also suited for recombinant production in microbial cells via fusion to carrier proteins [17,18] or through tandem arrangements [19][20][21]. Of course, the recombinant biofabrication of AMPs in prokaryotic cell factories offers, as in the case of any other protein drug [22,23], fully scalable, ecofriendly, and cost-effective methodologies [24][25][26][27] compatible with a sustainable circular economy [27]. Also, recombinant AMPs benefit from the generic properties of protein drugs, namely, excellent biocompatibility and biodegradability and the possibility of functional and structural adjustment through conventional genetic engineering [28,29].…”

Section: Introductionmentioning

confidence: 99%

Efficient Delivery of Antimicrobial Peptides in an Innovative, Slow-Release Pharmacological Formulation

Serna,

López-Laguna,

Aceituno

et al. 2023

Pharmaceutics

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Both nanostructure and multivalency enhance the biological activities of antimicrobial peptides (AMPs), whose mechanism of action is cooperative. In addition, the efficacy of a particular AMP should benefit from a steady concentration at the local place of action and, therefore, from a slow release after a dynamic repository. In the context of emerging multi-resistant bacterial infections and the urgent need for novel and effective antimicrobial drugs, we tested these concepts through the engineering of four AMPs into supramolecular complexes as pharmacological entities. For that purpose, GWH1, T22, Pt5, and PaD, produced as GFP or human nidogen-based His-tagged fusion proteins, were engineered as self-assembling oligomeric nanoparticles ranging from 10 to 70 nm and further packaged into nanoparticle-leaking submicron granules. Since these materials slowly release functional nanoparticles during their time-sustained unpacking, they are suitable for use as drug depots in vivo. In this context, a particular AMP version (GWH1-NIDO-H6) was selected for in vivo validation in a zebrafish model of a complex bacterial infection. The GWH1-NIDO-H6-secreting protein granules are protective in zebrafish against infection by the multi-resistant bacterium Stenotrophomonas maltophilia, proving the potential of innovative formulations based on nanostructured and slowly released recombinant AMPs in the fight against bacterial infections.

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“… 5 Moreover, the production of xylanase by A. niger offers the advantages of high yield, protein post-translational modification, strong extracellular secretion, and safety. 6 The technology of immobilized fermentation was developed on the basis of usage of an immobilized enzyme in the 1960s and 1970s. 7 As early as in 1995, Kang et al studied immobilized fermentation of A. niger and reported that the lignocellulase activity was significantly higher for immobilized cells in a bubble column than for a shake-flask culture.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, the production of xylanase by A. niger offers the advantages of high yield, protein post-translational modification, strong extracellular secretion, and safety . The technology of immobilized fermentation was developed on the basis of usage of an immobilized enzyme in the 1960s and 1970s .…”

Section: Introductionmentioning

confidence: 99%

Biofilm-Related, Time-Series Transcriptome and Genome Sequencing in Xylanase-ProducingAspergillus nigerSJ1

Sun

et al. 2020

ACS Omega

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In this study, we found that biofilm formation is a critical factor affecting the activity of Aspergillus niger SJ1 xylanase. Xylanase activity increased 8.8% from 1046.88 to 1147.74 U/mL during A. niger SJ1 immobilized fermentation with biofilm formation. Therefore, we carried out the work of genomic analysis and biofilm-related time-series transcriptome analysis of A. niger SJ1 for better understanding of the ability of A. niger SJ to produce xylanase and biofilm formation. Genome annotation results revealed a complete biofilm polysaccharide component synthesis pathway in A. niger SJ1 and five proteins regarding xylanase synthesis. In addition, results of transcriptome analysis revealed that the genes involved in the synthesis of cell wall polysaccharides and amino acid anabolism were highly expressed in the biofilm. Furthermore, the expression levels of major genes in the gluconeogenesis pathway and mitogen-activated protein kinase pathway were examined.

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Liquefied wheat bran as carbon source and inducer in high-solids submerged cultivation of Aspergillus niger for xylanase production

Cited by 14 publications

References 36 publications

Factors that influence the enzymatic hydrolysis of agricultural wastes for ethanol production: a review

Factors that influence the enzymatic hydrolysis of agricultural wastes for ethanol production: a review

Efficient Delivery of Antimicrobial Peptides in an Innovative, Slow-Release Pharmacological Formulation

Biofilm-Related, Time-Series Transcriptome and Genome Sequencing in Xylanase-ProducingAspergillus nigerSJ1

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