Liquid and Freeze‐Preservation of Dog Red Blood Cells

Contreras, T. J.; Lindberg, Joel; Lowrie, G. B.; Pivacek, Linda E.; Austin, R. M.; Vecchione, John J.; Valeri, C. R.

doi:10.1046/j.1537-2995.1979.19379204209.x

Cited by 14 publications

(30 citation statements)

References 263 publications

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“…The changes detected to be due to the effects of the duration of the storage period following freezing revealed similar results to those carried out on human blood products (Contreras et al 1979;Valeri et al 2000;Kim et al 2004;Lecak et al 2004;Sen and Khetarpal 2013). The reduction in 2,3-DPG concentrations could be associated with the decrease in pH values as a result of hydrogen ion accumulation due to the conversion of glucose into lactate (Hess and Greenwalt 2002;Holme 2005).…”

Section: Discussionsupporting

confidence: 72%

See 1 more Smart Citation

Freezing and storage of leukodepleted erythrocyte suspensions

Bala¹,

Eraslan²,

Akyazı³

et al. 2016

Vet. Med. - Czech

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Studies on the frozen storage of human blood products have benefited veterinary transfusion medicine in recent years, but the long-term cryopreservation of canine red blood cells (RBCs) has not yet been thoroughly investigated. Further, no studies are available with respect to the frozen storage of leukocyte-depleted canine red blood cells (LD-RBCs). The objective of the current study was to investigate time-dependent effects of long-term frozen storage on leukocyte-depleted canine RBCs. Twelve healthy adult dogs meeting the criteria for blood transfusion were used in the study. Whole blood samples (450 ± 45 ml) collected from each dog were centrifuged for 5 min at 22 °C and 4200 × g in a cryogenic microcentrifuge and concentrated RBC (pRBC) suspensions were obtained. Leukocyte depletion was achieved by filtration (2.6 log 10 ). Then, the filtrated samples were prewashed three times in 0.9% NaCl solution and were allocated into three subgroups to be evaluated at three different time points (Day 0, Month 4 and Month 6). The samples for cryopreservation were subjected to glycerolisation and then stored at -80 °C for 4-and 6-month periods. At the end of this period pRBC units were thawed by manual agitation in a water bath maintained at 36-38 °C, centrifuged and then washed in a consecutive series of 12%, 1.6% and 0.9% of NaCl + 0.2 dextrose solutions. 2,3-Diphosphoglycerate (2,3-DPG), adenosine triphosphate (ATP), supernatant haemoglobin (SupHb), sodium (Na + ) and potassium (K + ) levels, residual glycerol concentrations and haemograms of thawed and deglycerolised pRBC samples were evaluated together with those of Day 0. Sterility tests were performed on all samples for bacterial contamination. No statistically significant differences were noted except for Hct and SupHb levels. No bacterial contamination was noted in any of the samples on the basis of sterility tests. It was found that the described glycerolisation procedure could be a method of choice in the cryopreservation of leukocyte-depleted pRBCs (LD-pRBCs) since no negative effect was observed on the quality of the products and long-term frozen storage did not cause RBC destruction.

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Section: Discussionsupporting

confidence: 72%

“…However, there is only a limited number of studies with respect to presumptive alterations caused by frozen storage of canine erythrocytes via glycerolisation (Contreras et al 1979;Kim et al 2004;Kim et al 2007) Furthermore, there is no data available in terms of long-term storage of LD canine pRBC products.…”

mentioning

confidence: 99%

Freezing and storage of leukodepleted erythrocyte suspensions

Bala¹,

Eraslan²,

Akyazı³

et al. 2016

Vet. Med. - Czech

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“…We mimicked the 20% (w/v) glycerol freezing and deglycerolization procedures described by Contreras et al [2], where they added and removed glycerol in a step-wise manner to reduce osmotic damage. The 30 min saline stability mirrors the in vivo 24 hr post-transfusion survival rate [24].…”

Section: Discussionmentioning

confidence: 99%

“…Freezing, thawing and washing procedures for 20% (w/v) glycerol: The 20% (w/v) glycerol cryopreservation procedure was similar to the freezing and deglycerolization procedures described by Contreras et al [2], except that the equilibration time for deglycerolization was extended to 5 min. Thirty milliliters of glycerolized RBCs were transferred to a freezing bag (Cryogenic Storage Container, CharterMed Inc., Lakewood, NJ, U.S.A.).…”

Section: Methodsmentioning

confidence: 99%

A Comparative Study of the Effects of Glycerol and Hydroxyethyl Starch in Canine Red Blood Cell Cryopreservation

et al. 2004

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ABSTRACT. Hydroxyethyl starch (HES) is a nonpenetrating extracellular cryoprotectant. In contrast to glycerol, it does not require laborintensive removal from thawed red blood cells (RBCs) prior to transfusion. In this study, we compared glycerol and HES, and assessed HES as a substitute for glycerol in cryopreserved canine RBCs. The RBCs were preserved for 2 months in liquid nitrogen using a 20% (w/v) glycerol solution, and variable concentrations of HES solution. We evaluated the two cryoprotectants by the percentage of postthaw hemolysis from the total free hemoglobin, saline stability, osmotic fragility, and by observing the erythrocyte morphology using a scanning electron microscope after thawing. The optimal concentration of HES was 12.5% (w/v) for the cryopreservation of canine RBCs. The thaw hemolysis, saline stability, and osmotic fragility index were 25.6 ± 4.7%, 87.8 ± 6.9%, and 0.445 ± 0.024% NaCl respectively. These parameters resemble the results of RBCs frozen in a 20% (w/v) glycerol solution, which are 24.7 ± 5.2%, 99.2 ± 0.1%, and 0.485 ± 0.023% NaCl respectively. From a morphological point of view, 12.5% (w/v) HES showed the best cryoprotection of RBCs compared to the other concentrations of HES. These results suggest that HES could be a possible substitute for glycerol for the cryopreservation of canine RBCs.

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“…The changes monitored due to the duration of the storage period following the freezing process were found not to be statistically significant (Table 1). The studies conducted on human (Valeri et al 2000, Lecak et al 2004) and canine RBCs (Contreras et al 1979, Kim et al 2004) revealed similar results (Sen and Khetarpal 2013).…”

Section: Discussionmentioning

confidence: 53%

The effects of freezing on long-term storage of canine erythrocytes

Bala¹,

Özcan²

2016

Polish Journal of Veterinary Sciences

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Human medicine studies have so far demonstrated that erythrocytes may be preserved and stored at low temperatures for decades retaining their metabolic and biochemical properties. However, detailed studies regarding this problem are not yet available in veterinary medicine. Therefore, the objective of the current study was to investigate time-dependent effects of long-term frozen storage of canine red blood cells.Twelve healthy adult dogs meeting the criteria for blood transfusion were used in the study. Whole blood samples (450 ± 45 ml) collected from each dog were centrifuged by a cryogenic microcentrifuge and packed RBC suspensions were obtained. The samples were prewashed three times in 0.9% NaCl solution and were allocated into three groups to be evaluated at three different time points (day 0 and month 4 and 6). The samples to be frozen were subjected to glycerolization and then stored at -80 o C for 4 and 6-month periods. At the end of this period the packed RBC samples were thawed, centrifuged and then washed in a consecutive series of dextrose solutions. 2,3-Diphosphoglycerate (2,3-DPG), Adenosin triphosphate (ATP), supernatant hemoglobin (SupHb), sodium (Na + ) and potassium (K + ) levels, residual glycerol concentrations and hemograms were evaluated and compared. Sterility tests were performed on all samples for bacterial contamination. A statistically significant decrease was noted in potassium levels, which was the natural outcome of deglycerolization process. No significant change was observed in terms of other parameters due based on different time points. In conclusion, long-term frozen storage had no negative effect on the quality parameters of canine erythrocytes.

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Liquid and Freeze‐Preservation of Dog Red Blood Cells

Cited by 14 publications

References 263 publications

Freezing and storage of leukodepleted erythrocyte suspensions

Freezing and storage of leukodepleted erythrocyte suspensions

A Comparative Study of the Effects of Glycerol and Hydroxyethyl Starch in Canine Red Blood Cell Cryopreservation

The effects of freezing on long-term storage of canine erythrocytes

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