Liquid and Freeze Preservation of Human Red Blood Cells

Barrêto-Júnior

Veterinary Clinical Pathol

et al. 2012

Section: Pcv MCV Rbc Count and Plasma Hemoglobin Concentration In mentioning

confidence: 99%

Evaluation of hematologic, blood gas, and select biochemical variables in ovine whole blood stored in CPDA‐1 bags

Barrêto-Júnior

Veterinary Clinical Pathol

et al. 2012

“…There have been a number of studies on the restoration of ACD-blood with decreased oxygen transport function (see Valeri, 1974). The present study describes (i) the functional restoration of ACD-stored blood by an aUosteric effector, PLP, (ii) some factors influencing PLP uptake by ACD-erythrocytes, and (iii) the effect of the PLP-loading on the intracellular nucleotides content.…”

Section: Discussionmentioning

confidence: 95%

Functional Restoration of ACD‐Blood by Pyridoxal 5′‐Phosphate

et al. 1980

Successful application of pyridoxal 5'-phosphate (PLP) to restore the oxygen transport function of ACD-stored blood is described. PLP is readily incorporated into ACD-erythrocytes by both carrier-mediated transport (in which ATP may participate) and passive diffusion. Plasma proteins (up to 2.5%) and inorganic phosphate (up to 40 mM) do not affect the incorporation of PLP, though more than 25 mM inorganic phosphate is necessary for the maintenance of ATP levels. Increasing the PLP concentration and/or decreasing the packed cell volume in the medium, increases the incorporation of PLP. Incubation of erythrocytes with PLP at pH 7.0 is most suitable for incorporation of PLP and the maintenance of ATP levels. PLP incorporated into erythrocytes restores the oxygen transport function of the ACD-erythrocytes, though decreased haem--haem interaction is observed. A procedure for the clinical application of PLP-loaded erythrocytes is suggested.

“…Tris serves as a base reserve to balance acidity due to lactic acid production during storage, and C02 compensates for the alkalinity due to the Tris solution as long as lactic acid produc tion is insufficient to neutralize it. The step wise modification of the gas atmosphere is 22,29,33,34]. In our case, two potential supplementary causes of increased hemol ysis, shaking and the use of a vacuum [27], could have been contributing factors al tering erythrocyte fragility.…”

Section: Discussionmentioning

confidence: 99%

Maintenance of 2,3-DPG and ATP Levels in Blood Stored for 30 Days at a Constant pH Obtained by Variation of C0(2)

Mendonca¹,

Appel²,

Bidault³

et al. 1978

Vox Sanguinis

A novel procedure for storing blood at a controlled pH consists in collecting blood in a pH 8.20, Tris-CPD solution and storing it in a special recipient including a gas permeable membrane under a CO(2) atmosphere. The recipient is placed in an atmosphere of variable CO(2)content, so that the initial alkalinity of the preservative is balanced by dissolved CO(2), the proportion of which is diminished when lactate production increases with storage. 2,3-DPG and ATP were studied at three different pH levels of approximately 7.25, 7.45, and 7.65 at 4°C. The best pH for the simultaneous maintenance of 2,3-DPG and ATP was 7.65. Under these conditions, 2,3-DPG is maintained at its initial level and ATP at 55% of its initial level at the 30th day. Lactate production is linear and hemolysis moderate.